Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy
The transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/4585169 |
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| author | Danna Ye Philip Heraud Rangsun Parnpai Tong Li |
| author_facet | Danna Ye Philip Heraud Rangsun Parnpai Tong Li |
| author_sort | Danna Ye |
| collection | DOAJ |
| description | The transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage in rat model. Synchrotron radiation Fourier-transform infrared (SR-FTIR) microspectroscopy was applied to investigate changes in the macromolecular composition. Transplantation of rBM-MSC-DSCs into liver-injured rats restored their serum albumin level and significantly suppressed transaminase activity as well as the morphological manifestations of liver disease. The regenerative effects of rBM-MSC-DSCs were corroborated unequivocally by the phenotypic difference analysis between liver tissues revealed by infrared spectroscopy. Spectroscopic changes in the spectral region from 1190–970 cm−1 (bands with absorbance maxima at 1150 cm−1, 1081 cm−1, and 1026 cm−1) indicated decreased levels of carbohydrates, in rBM-MSC-DSC-transplanted livers, compared with untreated and rBM-MSC--transplanted animals. Principal component analysis (PCA) of spectra acquired from liver tissue could readily discriminate rBM-MSC-DSC-transplanted animals from the untreated and rBM-MSC-transplanted animals. We conclude that the transplantation of rBM-MSC-DSCs effectively treats liver disease in rats and SR-FTIR microspectroscopy provides important insights into the fundamental biochemical alterations induced by the stem-derived cell transplantation, including an objective “signature” of the regenerative effects of stem cell therapy upon liver injury. |
| format | Article |
| id | doaj-art-a79c851aeddf437e8e2c102225ee86f7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a79c851aeddf437e8e2c102225ee86f72025-02-03T01:01:44ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/45851694585169Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR SpectroscopyDanna Ye0Philip Heraud1Rangsun Parnpai2Tong Li3Department of Reproductive Medical Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, AustraliaEmbryo Technology and Stem Cell Research Center, School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima, ThailandSchool of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, ChinaThe transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage in rat model. Synchrotron radiation Fourier-transform infrared (SR-FTIR) microspectroscopy was applied to investigate changes in the macromolecular composition. Transplantation of rBM-MSC-DSCs into liver-injured rats restored their serum albumin level and significantly suppressed transaminase activity as well as the morphological manifestations of liver disease. The regenerative effects of rBM-MSC-DSCs were corroborated unequivocally by the phenotypic difference analysis between liver tissues revealed by infrared spectroscopy. Spectroscopic changes in the spectral region from 1190–970 cm−1 (bands with absorbance maxima at 1150 cm−1, 1081 cm−1, and 1026 cm−1) indicated decreased levels of carbohydrates, in rBM-MSC-DSC-transplanted livers, compared with untreated and rBM-MSC--transplanted animals. Principal component analysis (PCA) of spectra acquired from liver tissue could readily discriminate rBM-MSC-DSC-transplanted animals from the untreated and rBM-MSC-transplanted animals. We conclude that the transplantation of rBM-MSC-DSCs effectively treats liver disease in rats and SR-FTIR microspectroscopy provides important insights into the fundamental biochemical alterations induced by the stem-derived cell transplantation, including an objective “signature” of the regenerative effects of stem cell therapy upon liver injury.http://dx.doi.org/10.1155/2017/4585169 |
| spellingShingle | Danna Ye Philip Heraud Rangsun Parnpai Tong Li Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy Stem Cells International |
| title | Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy |
| title_full | Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy |
| title_fullStr | Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy |
| title_full_unstemmed | Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy |
| title_short | Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy |
| title_sort | reversal of experimental liver damage after transplantation of stem derived cells detected by ftir spectroscopy |
| url | http://dx.doi.org/10.1155/2017/4585169 |
| work_keys_str_mv | AT dannaye reversalofexperimentalliverdamageaftertransplantationofstemderivedcellsdetectedbyftirspectroscopy AT philipheraud reversalofexperimentalliverdamageaftertransplantationofstemderivedcellsdetectedbyftirspectroscopy AT rangsunparnpai reversalofexperimentalliverdamageaftertransplantationofstemderivedcellsdetectedbyftirspectroscopy AT tongli reversalofexperimentalliverdamageaftertransplantationofstemderivedcellsdetectedbyftirspectroscopy |