Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L.
Abstract Glyphosate (Gly) is a widely used herbicide for weed control in agriculture, but it can also adversely affect crops by impairing growth, reducing yield, and disrupting nutrient uptake, while inducing toxicity. Therefore, adopting integrated eco-friendly approaches and understanding the mech...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-87024-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585730585526272 |
|---|---|
| author | Probir Kumar Mittra Md Atikur Rahman Swapan Kumar Roy Soo-Jeong Kwon Abhik Mojumdar Sung Ho Yun Kun Cho Seong-Woo Cho Meiliang Zhou Tomoyuki Katsube-Tanaka Sun-Hee Woo |
| author_facet | Probir Kumar Mittra Md Atikur Rahman Swapan Kumar Roy Soo-Jeong Kwon Abhik Mojumdar Sung Ho Yun Kun Cho Seong-Woo Cho Meiliang Zhou Tomoyuki Katsube-Tanaka Sun-Hee Woo |
| author_sort | Probir Kumar Mittra |
| collection | DOAJ |
| description | Abstract Glyphosate (Gly) is a widely used herbicide for weed control in agriculture, but it can also adversely affect crops by impairing growth, reducing yield, and disrupting nutrient uptake, while inducing toxicity. Therefore, adopting integrated eco-friendly approaches and understanding the mechanisms of glyphosate tolerance in plants is crucial, as these areas remain underexplored. This study provides proteome insights into Si-mediated improvement of Gly-toxicity tolerance in Brassica napus. The proteome analysis identified a total of 4,407 proteins, of which 594 were differentially abundant, including 208 up-regulated and 386 down-regulated proteins. These proteins are associated with diverse biological processes in B. napus, including energy metabolism, antioxidant activity, signal transduction, photosynthesis, sulfur assimilation, cell wall functions, herbicide tolerance, and plant development. Protein-protein interactome analyses confirmed the involvement of six key proteins, including L-ascorbate peroxidase, superoxide dismutase, glutaredoxin-C2, peroxidase, glutathione peroxidase (GPX) 2, and peptide methionine sulfoxide reductase A3 which involved in antioxidant activity, sulfur assimilation, and herbicide tolerance, contributing to the resilience of B. napus against Gly toxicity. The proteomics insights into Si-mediated Gly-toxicity mitigation is an eco-friendly approach, and alteration of key molecular processes opens a new perspective of multi-omics-assisted B. napus breeding for enhancing herbicide resistant oilseed crop production. |
| format | Article |
| id | doaj-art-a791b1711bfc42899da0cc09769cbe83 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a791b1711bfc42899da0cc09769cbe832025-01-26T12:32:32ZengNature PortfolioScientific Reports2045-23222025-01-0115112310.1038/s41598-025-87024-5Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L.Probir Kumar Mittra0Md Atikur Rahman1Swapan Kumar Roy2Soo-Jeong Kwon3Abhik Mojumdar4Sung Ho Yun5Kun Cho6Seong-Woo Cho7Meiliang Zhou8Tomoyuki Katsube-Tanaka9Sun-Hee Woo10Department of Crop Science, Chungbuk National UniversityABEx Bio-Research CenterCollege of Agricultural Sciences, IUBAT—International University of Business Agriculture and TechnologyDepartment of Crop Science, Chungbuk National UniversityDigital Omics Research Center, Ochang Center, Korea Basic Science InstituteDigital Omics Research Center, Ochang Center, Korea Basic Science InstituteDigital Omics Research Center, Ochang Center, Korea Basic Science InstituteDepartment of Agronomy and Medicinal Plant Resources, Gyeongsang National UniversityInstitute of Crop Sciences, Chinese Academy of Agricultural SciencesGraduate School of Agriculture, Kyoto UniversityDepartment of Crop Science, Chungbuk National UniversityAbstract Glyphosate (Gly) is a widely used herbicide for weed control in agriculture, but it can also adversely affect crops by impairing growth, reducing yield, and disrupting nutrient uptake, while inducing toxicity. Therefore, adopting integrated eco-friendly approaches and understanding the mechanisms of glyphosate tolerance in plants is crucial, as these areas remain underexplored. This study provides proteome insights into Si-mediated improvement of Gly-toxicity tolerance in Brassica napus. The proteome analysis identified a total of 4,407 proteins, of which 594 were differentially abundant, including 208 up-regulated and 386 down-regulated proteins. These proteins are associated with diverse biological processes in B. napus, including energy metabolism, antioxidant activity, signal transduction, photosynthesis, sulfur assimilation, cell wall functions, herbicide tolerance, and plant development. Protein-protein interactome analyses confirmed the involvement of six key proteins, including L-ascorbate peroxidase, superoxide dismutase, glutaredoxin-C2, peroxidase, glutathione peroxidase (GPX) 2, and peptide methionine sulfoxide reductase A3 which involved in antioxidant activity, sulfur assimilation, and herbicide tolerance, contributing to the resilience of B. napus against Gly toxicity. The proteomics insights into Si-mediated Gly-toxicity mitigation is an eco-friendly approach, and alteration of key molecular processes opens a new perspective of multi-omics-assisted B. napus breeding for enhancing herbicide resistant oilseed crop production.https://doi.org/10.1038/s41598-025-87024-5Key antioxidant proteinsEco-friendly approachHerbicide toxicityHerbicide toleranceLabel-free proteomics |
| spellingShingle | Probir Kumar Mittra Md Atikur Rahman Swapan Kumar Roy Soo-Jeong Kwon Abhik Mojumdar Sung Ho Yun Kun Cho Seong-Woo Cho Meiliang Zhou Tomoyuki Katsube-Tanaka Sun-Hee Woo Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L. Scientific Reports Key antioxidant proteins Eco-friendly approach Herbicide toxicity Herbicide tolerance Label-free proteomics |
| title | Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L. |
| title_full | Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L. |
| title_fullStr | Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L. |
| title_full_unstemmed | Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L. |
| title_short | Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L. |
| title_sort | proteomic analysis reveals the roles of silicon in mitigating glyphosate induced toxicity in brassica napus l |
| topic | Key antioxidant proteins Eco-friendly approach Herbicide toxicity Herbicide tolerance Label-free proteomics |
| url | https://doi.org/10.1038/s41598-025-87024-5 |
| work_keys_str_mv | AT probirkumarmittra proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT mdatikurrahman proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT swapankumarroy proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT soojeongkwon proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT abhikmojumdar proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT sunghoyun proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT kuncho proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT seongwoocho proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT meiliangzhou proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT tomoyukikatsubetanaka proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl AT sunheewoo proteomicanalysisrevealstherolesofsiliconinmitigatingglyphosateinducedtoxicityinbrassicanapusl |