Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258
The fundamental goal of our investigation is to employ a sustainable synthesis method for zinc oxide nanoparticles (ZnO NPs), utilizing lactic acid bacteria isolated from curd as the key biological agent. Bacteria function as agents for both reduction and capping processes, which aids the synthesis...
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| Language: | English |
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Beilstein-Institut
2025-01-01
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| Series: | Beilstein Journal of Nanotechnology |
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| Online Access: | https://doi.org/10.3762/bjnano.16.8 |
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| author | Prashantkumar Siddappa Chakra Aishwarya Banakar Shriram Narayan Puranik Vishwas Kaveeshwar C. R. Ravikumar Devaraja Gayathri |
| author_facet | Prashantkumar Siddappa Chakra Aishwarya Banakar Shriram Narayan Puranik Vishwas Kaveeshwar C. R. Ravikumar Devaraja Gayathri |
| author_sort | Prashantkumar Siddappa Chakra |
| collection | DOAJ |
| description | The fundamental goal of our investigation is to employ a sustainable synthesis method for zinc oxide nanoparticles (ZnO NPs), utilizing lactic acid bacteria isolated from curd as the key biological agent. Bacteria function as agents for both reduction and capping processes, which aids the synthesis of ZnO NPs. Various characterization techniques including XRD, FTIR, UV–vis, TEM, SEM-EDX, and zeta potential measurements were employed to analyze the morphology, dimensions, and elemental composition of the generated nanoparticles. The experimental outcomes confirmed the presence of hexagonal wurtzite-structured ZnO NPs with an average size of 10 nm. The colloidal system demonstrated excellent stability with a zeta potential of −60 mV. Furthermore, the synthesized nanoparticles displayed significant antibacterial activity against selected human pathogens, with the biggest inhibition zone observed against Staphylococcus aureus (22 ± 0.57 mm) and the smallest inhibition zone observed against Salmonella enterica serovar typhi (3 ± 1 mm). MTT assay revealed the promising antiproliferative potential of ZnO NPs, with an average IC50 value of 98.53 µg/mL. Additionally, the NPs were photocatalytically and electrochemically analyzed, indicating their potential use in cancer research as well as in coating and drug delivery applications. |
| format | Article |
| id | doaj-art-a78a14c3f94e465bb507612a9bb1c601 |
| institution | Kabale University |
| issn | 2190-4286 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj-art-a78a14c3f94e465bb507612a9bb1c6012025-02-03T09:10:12ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862025-01-01161788910.3762/bjnano.16.82190-4286-16-8Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258Prashantkumar Siddappa Chakra0Aishwarya Banakar1Shriram Narayan Puranik2Vishwas Kaveeshwar3C. R. Ravikumar4Devaraja Gayathri5Department of Studies in Microbiology, Davangere University, Davangere, Karnataka, 577007, India Central Research Laboratory, S.D.M College of Medical Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India Central Research Laboratory, S.D.M College of Medical Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India Central Research Laboratory, S.D.M College of Medical Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India Research Centre, Department of Science, East West Institute of Technology, Bangalore, 560091, IndiaDepartment of Studies in Microbiology, Davangere University, Davangere, Karnataka, 577007, India The fundamental goal of our investigation is to employ a sustainable synthesis method for zinc oxide nanoparticles (ZnO NPs), utilizing lactic acid bacteria isolated from curd as the key biological agent. Bacteria function as agents for both reduction and capping processes, which aids the synthesis of ZnO NPs. Various characterization techniques including XRD, FTIR, UV–vis, TEM, SEM-EDX, and zeta potential measurements were employed to analyze the morphology, dimensions, and elemental composition of the generated nanoparticles. The experimental outcomes confirmed the presence of hexagonal wurtzite-structured ZnO NPs with an average size of 10 nm. The colloidal system demonstrated excellent stability with a zeta potential of −60 mV. Furthermore, the synthesized nanoparticles displayed significant antibacterial activity against selected human pathogens, with the biggest inhibition zone observed against Staphylococcus aureus (22 ± 0.57 mm) and the smallest inhibition zone observed against Salmonella enterica serovar typhi (3 ± 1 mm). MTT assay revealed the promising antiproliferative potential of ZnO NPs, with an average IC50 value of 98.53 µg/mL. Additionally, the NPs were photocatalytically and electrochemically analyzed, indicating their potential use in cancer research as well as in coating and drug delivery applications.https://doi.org/10.3762/bjnano.16.8anticancergreen synthesislactic acid bacteriananoparticleszinc oxide |
| spellingShingle | Prashantkumar Siddappa Chakra Aishwarya Banakar Shriram Narayan Puranik Vishwas Kaveeshwar C. R. Ravikumar Devaraja Gayathri Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 Beilstein Journal of Nanotechnology anticancer green synthesis lactic acid bacteria nanoparticles zinc oxide |
| title | Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 |
| title_full | Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 |
| title_fullStr | Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 |
| title_full_unstemmed | Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 |
| title_short | Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 |
| title_sort | characterization of zno nanoparticles synthesized using probiotic lactiplantibacillus plantarum gp258 |
| topic | anticancer green synthesis lactic acid bacteria nanoparticles zinc oxide |
| url | https://doi.org/10.3762/bjnano.16.8 |
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