Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo
It still remains a major challenge to repair large bone defects in the orthopaedic surgery. In previous studies, a nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite, similar to natural bone in both composition and structure, has been prepared. It could repair small sized bone defe...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2014/153453 |
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| author | Hai Wang Xiao Chang Guixing Qiu Fuzhai Cui Xisheng Weng Baozhong Zhang Xiaojie Lian Zhihong Wu |
| author_facet | Hai Wang Xiao Chang Guixing Qiu Fuzhai Cui Xisheng Weng Baozhong Zhang Xiaojie Lian Zhihong Wu |
| author_sort | Hai Wang |
| collection | DOAJ |
| description | It still remains a major challenge to repair large bone defects in the orthopaedic surgery. In previous studies, a nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite, similar to natural bone in both composition and structure, has been prepared. It could repair small sized bone defects, but they were restricted to repair a large defect due to the lack of oxygen and nutrition supply for cell survival without vascularization. The aim of the present study was to investigate whether nHAC/PLA composites could be vascularized in vivo. Composites were implanted intramuscularly in the groins of rabbits for 2, 6, or 10 weeks (n=5×3). After removing, the macroscopic results showed that there were lots of rich blood supply tissues embracing the composites, and the volumes of tissue were increasing as time goes on. In microscopic views, blood vessels and vascular sprouts could be observed, and microvessel density (MVD) of the composites trended to increase over time. It suggested that nHAC/PLA composites could be well vascularized by implanting in vivo. In the future, it would be possible to generate vascular pedicle bone substitutes with nHAC/PLA composites for grafting. |
| format | Article |
| id | doaj-art-a775384acaf34e64898a9df30e4387c1 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-a775384acaf34e64898a9df30e4387c12025-02-03T05:47:07ZengWileyInternational Journal of Polymer Science1687-94221687-94302014-01-01201410.1155/2014/153453153453Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In VivoHai Wang0Xiao Chang1Guixing Qiu2Fuzhai Cui3Xisheng Weng4Baozhong Zhang5Xiaojie Lian6Zhihong Wu7Department of Orthopaedics, Peking Union Medical College Hospital, No. 1 Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedics, Peking Union Medical College Hospital, No. 1 Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedics, Peking Union Medical College Hospital, No. 1 Shuaifuyuan Hutong, Beijing 100730, ChinaBiomaterials Laboratory, Department of Material Science & Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Orthopaedics, Peking Union Medical College Hospital, No. 1 Shuaifuyuan Hutong, Beijing 100730, ChinaDepartment of Orthopaedics, Peking Union Medical College Hospital, No. 1 Shuaifuyuan Hutong, Beijing 100730, ChinaBiomaterials Laboratory, Department of Material Science & Engineering, Tsinghua University, Beijing 100084, ChinaDepartment of Orthopaedics, Peking Union Medical College Hospital, No. 1 Shuaifuyuan Hutong, Beijing 100730, ChinaIt still remains a major challenge to repair large bone defects in the orthopaedic surgery. In previous studies, a nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite, similar to natural bone in both composition and structure, has been prepared. It could repair small sized bone defects, but they were restricted to repair a large defect due to the lack of oxygen and nutrition supply for cell survival without vascularization. The aim of the present study was to investigate whether nHAC/PLA composites could be vascularized in vivo. Composites were implanted intramuscularly in the groins of rabbits for 2, 6, or 10 weeks (n=5×3). After removing, the macroscopic results showed that there were lots of rich blood supply tissues embracing the composites, and the volumes of tissue were increasing as time goes on. In microscopic views, blood vessels and vascular sprouts could be observed, and microvessel density (MVD) of the composites trended to increase over time. It suggested that nHAC/PLA composites could be well vascularized by implanting in vivo. In the future, it would be possible to generate vascular pedicle bone substitutes with nHAC/PLA composites for grafting.http://dx.doi.org/10.1155/2014/153453 |
| spellingShingle | Hai Wang Xiao Chang Guixing Qiu Fuzhai Cui Xisheng Weng Baozhong Zhang Xiaojie Lian Zhihong Wu Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo International Journal of Polymer Science |
| title | Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo |
| title_full | Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo |
| title_fullStr | Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo |
| title_full_unstemmed | Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo |
| title_short | Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo |
| title_sort | vascularization of nanohydroxyapatite collagen poly l lactic acid composites by implanting intramuscularly in vivo |
| url | http://dx.doi.org/10.1155/2014/153453 |
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