A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i>
While the rapid rise in bioinformatics has facilitated the identification of the domains and functions of many proteins, some still have no domain annotation or largely uncharacterized functions. However, the biological roles of unknown proteins were not clear in oomycetes. An analysis of the <i&...
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2024-12-01
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| Series: | Journal of Fungi |
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| author | Xiaoran Du Yan Zeng Yiying Li Qin Peng Jianqiang Miao Xili Liu |
| author_facet | Xiaoran Du Yan Zeng Yiying Li Qin Peng Jianqiang Miao Xili Liu |
| author_sort | Xiaoran Du |
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| description | While the rapid rise in bioinformatics has facilitated the identification of the domains and functions of many proteins, some still have no domain annotation or largely uncharacterized functions. However, the biological roles of unknown proteins were not clear in oomycetes. An analysis of the <i>Phytophthora sojae</i> genome database identified the protein Ps495620, which has no domain annotations and functional predictions in <i>Phytophthora</i>. This study used a CRISPR/Cas9-mediated gene replacement system to knock out <i>Ps495620</i> to elucidate its function. The <i>Ps495620</i>-knockout mutants exhibited significantly increased oospore production and decreased sporangium formation compared to the wild-type strain P6497. Transcriptomics showed that it is a key regulator of nitrogen, pyruvate, ascorbate, and adorate metabolism in <i>P. sojae</i>. Our findings indicate that Ps495620 is critical in regulating sporangium formation and oospore production in <i>P. sojae</i>. |
| format | Article |
| id | doaj-art-a77161f169024fe8a3b6659acf6e3093 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-a77161f169024fe8a3b6659acf6e30932025-01-24T13:37:13ZengMDPI AGJournal of Fungi2309-608X2024-12-011111210.3390/jof11010012A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i>Xiaoran Du0Yan Zeng1Yiying Li2Qin Peng3Jianqiang Miao4Xili Liu5State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaWhile the rapid rise in bioinformatics has facilitated the identification of the domains and functions of many proteins, some still have no domain annotation or largely uncharacterized functions. However, the biological roles of unknown proteins were not clear in oomycetes. An analysis of the <i>Phytophthora sojae</i> genome database identified the protein Ps495620, which has no domain annotations and functional predictions in <i>Phytophthora</i>. This study used a CRISPR/Cas9-mediated gene replacement system to knock out <i>Ps495620</i> to elucidate its function. The <i>Ps495620</i>-knockout mutants exhibited significantly increased oospore production and decreased sporangium formation compared to the wild-type strain P6497. Transcriptomics showed that it is a key regulator of nitrogen, pyruvate, ascorbate, and adorate metabolism in <i>P. sojae</i>. Our findings indicate that Ps495620 is critical in regulating sporangium formation and oospore production in <i>P. sojae</i>.https://www.mdpi.com/2309-608X/11/1/12<i>Phytophthora sojae</i>CRISPR/Cas9gene functionzoosporeoospore |
| spellingShingle | Xiaoran Du Yan Zeng Yiying Li Qin Peng Jianqiang Miao Xili Liu A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> Journal of Fungi <i>Phytophthora sojae</i> CRISPR/Cas9 gene function zoospore oospore |
| title | A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> |
| title_full | A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> |
| title_fullStr | A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> |
| title_full_unstemmed | A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> |
| title_short | A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> |
| title_sort | protein with unknown function ps495620 is critical for the sporulation and oospore production of i phytophthora sojae i |
| topic | <i>Phytophthora sojae</i> CRISPR/Cas9 gene function zoospore oospore |
| url | https://www.mdpi.com/2309-608X/11/1/12 |
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