Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells
Background. Aseptic loosening (AL) is a major complication of total joint replacement. Recent approaches to limiting AL have focused on inhibiting periprosthetic inflammation and osteoclastogenesis. Questions/Purposes. The purpose of this study was to determine the effects of erythromycin (EM) on po...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/107196 |
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| author | Yi Shen Weili Wang Xiaomiao Li David C. Markel Weiping Ren |
| author_facet | Yi Shen Weili Wang Xiaomiao Li David C. Markel Weiping Ren |
| author_sort | Yi Shen |
| collection | DOAJ |
| description | Background. Aseptic loosening (AL) is a major complication of total joint replacement. Recent approaches to limiting AL have focused on inhibiting periprosthetic inflammation and osteoclastogenesis. Questions/Purposes. The purpose of this study was to determine the effects of erythromycin (EM) on polymethylmethacrylate (PMMA) particle-challenged MC3T3 osteoblast precursor cells. Methods. MC3T3 cells were pretreated with EM (0–10 μg/mL) and then stimulated with PMMA (1 mg/mL). Cell viability was evaluated by both a lactate dehydrogenase (LDH) release assay and cell counts. Cell differentiation was determined by activity of alkaline phosphatase (ALP). Gene expression was measured via real-time quantitative RT-PCR. Results. We found that exposure to PMMA particles reduced cellular viability and osteogenetic potential in MC3T3 cell line. EM treatment mitigated the effects of PMMA particles on the proliferation, viability and differentiation of MC3T3 cells. PMMA decreased the gene expression of Runx2, osterix and osteocalcin, which can be partially restored by EM treatment. Furthermore, EM suppressed PMMA- induced increase of NF-κB gene expression. Conclusions. These data demonstrate that EM mitigates the effects of PMMA on MC3T3 cell viability and differentiation, in part through downregulation of NF-κB pathway. EM appeared to represent an anabolic agent on MC3T3 cells challenged with PMMA particles. |
| format | Article |
| id | doaj-art-a766c672d9954d188b619266e2ceee54 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a766c672d9954d188b619266e2ceee542025-02-03T05:51:49ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/107196107196Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 CellsYi Shen0Weili Wang1Xiaomiao Li2David C. Markel3Weiping Ren4Department of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, ChinaDepartment of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, ChinaDepartment of Orthopaedic, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, ChinaDepartment of Orthopedic Surgery, Providence Hospital & Detroit Medical Center Orthopedic Residency, Detroit, MI 48201, USADepartment of Orthopedic Surgery, Providence Hospital & Detroit Medical Center Orthopedic Residency, Detroit, MI 48201, USABackground. Aseptic loosening (AL) is a major complication of total joint replacement. Recent approaches to limiting AL have focused on inhibiting periprosthetic inflammation and osteoclastogenesis. Questions/Purposes. The purpose of this study was to determine the effects of erythromycin (EM) on polymethylmethacrylate (PMMA) particle-challenged MC3T3 osteoblast precursor cells. Methods. MC3T3 cells were pretreated with EM (0–10 μg/mL) and then stimulated with PMMA (1 mg/mL). Cell viability was evaluated by both a lactate dehydrogenase (LDH) release assay and cell counts. Cell differentiation was determined by activity of alkaline phosphatase (ALP). Gene expression was measured via real-time quantitative RT-PCR. Results. We found that exposure to PMMA particles reduced cellular viability and osteogenetic potential in MC3T3 cell line. EM treatment mitigated the effects of PMMA particles on the proliferation, viability and differentiation of MC3T3 cells. PMMA decreased the gene expression of Runx2, osterix and osteocalcin, which can be partially restored by EM treatment. Furthermore, EM suppressed PMMA- induced increase of NF-κB gene expression. Conclusions. These data demonstrate that EM mitigates the effects of PMMA on MC3T3 cell viability and differentiation, in part through downregulation of NF-κB pathway. EM appeared to represent an anabolic agent on MC3T3 cells challenged with PMMA particles.http://dx.doi.org/10.1155/2014/107196 |
| spellingShingle | Yi Shen Weili Wang Xiaomiao Li David C. Markel Weiping Ren Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells The Scientific World Journal |
| title | Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells |
| title_full | Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells |
| title_fullStr | Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells |
| title_full_unstemmed | Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells |
| title_short | Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells |
| title_sort | mitigative effect of erythromycin on pmma challenged preosteoblastic mc3t3 e1 cells |
| url | http://dx.doi.org/10.1155/2014/107196 |
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