Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform
Prostate cancer stands as the most diagnosed cancer in males and remains one of the leading causes of death among men in the United States. The progression of prostate cancer to a life-threatening state occurs upon metastasis, typically spreading to vital organs such as the liver, lungs, bones, and...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Biomedical Engineering Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667099225000015 |
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| author | Anjani Chavali Giles Fitzwilliams Adam Germain Sandra Khuon Young-tae Kim |
| author_facet | Anjani Chavali Giles Fitzwilliams Adam Germain Sandra Khuon Young-tae Kim |
| author_sort | Anjani Chavali |
| collection | DOAJ |
| description | Prostate cancer stands as the most diagnosed cancer in males and remains one of the leading causes of death among men in the United States. The progression of prostate cancer to a life-threatening state occurs upon metastasis, typically spreading to vital organs such as the liver, lungs, bones, and lymph nodes, where it sustains growth even in the absence of androgens. In this study, we employed a magnetic coculture device to investigate the interactions between androgen-independent prostate cancer (PC3) cells and healthy normal fibroblasts, aiming to discern their dynamics. Subsequently, the coculture was exposed to varying dosages of Fenbendazole to assess its efficacy differentially on healthy fibroblasts compared to androgen-independent prostate cells. Employing this straightforward coculture method, we observed significant growth, motility, and cluster formation of prostate cancer cells upon direct contact with surrounding fibroblasts. The impact of Fenbendazole was evident in its capacity to markedly diminish the growth and metastasis of prostate cancer cells relative to surrounding fibroblasts. Notably, our findings revealed that a dosage of 2.5 µM Fenbendazole significantly eradicated PC3 cells with minimal damage to surrounding fibroblasts, thus indicating its potential for prostate cancer treatment in-vivo models. |
| format | Article |
| id | doaj-art-a7598b98ebcd473d8b1aff53190bf237 |
| institution | Kabale University |
| issn | 2667-0992 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Engineering Advances |
| spelling | doaj-art-a7598b98ebcd473d8b1aff53190bf2372025-01-26T05:05:17ZengElsevierBiomedical Engineering Advances2667-09922025-06-019100144Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platformAnjani Chavali0Giles Fitzwilliams1Adam Germain2Sandra Khuon3Young-tae Kim4Department of Bioengineering, University of Texas at Arlington, Arlington, TX, USADepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, USA; Corresponding authors at: Associate Professor, Department of Bioengineering, University of Texas at Arlington, TX USA.Department of Bioengineering, University of Texas at Arlington, Arlington, TX, USADepartment of Nursing, University of Texas at Arlington, Arlington, TX, USADepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, USA; Department of Urology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Corresponding authors at: Associate Professor, Department of Bioengineering, University of Texas at Arlington, TX USA.Prostate cancer stands as the most diagnosed cancer in males and remains one of the leading causes of death among men in the United States. The progression of prostate cancer to a life-threatening state occurs upon metastasis, typically spreading to vital organs such as the liver, lungs, bones, and lymph nodes, where it sustains growth even in the absence of androgens. In this study, we employed a magnetic coculture device to investigate the interactions between androgen-independent prostate cancer (PC3) cells and healthy normal fibroblasts, aiming to discern their dynamics. Subsequently, the coculture was exposed to varying dosages of Fenbendazole to assess its efficacy differentially on healthy fibroblasts compared to androgen-independent prostate cells. Employing this straightforward coculture method, we observed significant growth, motility, and cluster formation of prostate cancer cells upon direct contact with surrounding fibroblasts. The impact of Fenbendazole was evident in its capacity to markedly diminish the growth and metastasis of prostate cancer cells relative to surrounding fibroblasts. Notably, our findings revealed that a dosage of 2.5 µM Fenbendazole significantly eradicated PC3 cells with minimal damage to surrounding fibroblasts, thus indicating its potential for prostate cancer treatment in-vivo models.http://www.sciencedirect.com/science/article/pii/S2667099225000015Magnetic coculture platformCancer-normal healthy cell interfaceMetastasisAndrogen-independent prostate cancerAnd drug discovery |
| spellingShingle | Anjani Chavali Giles Fitzwilliams Adam Germain Sandra Khuon Young-tae Kim Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform Biomedical Engineering Advances Magnetic coculture platform Cancer-normal healthy cell interface Metastasis Androgen-independent prostate cancer And drug discovery |
| title | Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform |
| title_full | Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform |
| title_fullStr | Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform |
| title_full_unstemmed | Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform |
| title_short | Exploring therapeutic strategies for androgen-independent prostate cancer using a magnetic coculture platform |
| title_sort | exploring therapeutic strategies for androgen independent prostate cancer using a magnetic coculture platform |
| topic | Magnetic coculture platform Cancer-normal healthy cell interface Metastasis Androgen-independent prostate cancer And drug discovery |
| url | http://www.sciencedirect.com/science/article/pii/S2667099225000015 |
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