A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy
The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The resu...
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Tsinghua University Press
2017-12-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453017301027 |
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| author | Cheng-Chin Wang Shi-Ying Huang Shu-Hung Huang Zhi-Hong Wen Jyun-Ying Huang Wen-Sheng Liu Hui-Min David Wang |
| author_facet | Cheng-Chin Wang Shi-Ying Huang Shu-Hung Huang Zhi-Hong Wen Jyun-Ying Huang Wen-Sheng Liu Hui-Min David Wang |
| author_sort | Cheng-Chin Wang |
| collection | DOAJ |
| description | The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The results reveal that a higher rotational speed exhibit a higher cell density, and the increasing in the amount of inoculum amount show a slight augment on the growth of R. sphaeroides WL-APD911.In the case of nitrogen sources adding, Lycogen⢠production was achieved with a 0.5 mM l-lysine supplementation. Moreover, the attention of Tween 80 presented a tremendous increase in the secondary metabolite. Response surface methodology (RSM) exhibited the optimization of medium supplements for Lycogen⢠invention is accomplished at molasses concentration of 10 g/L, yeast extract concentration of 40 g/L, 0.3% Tween 80 and NaCl concentration of 5 g/L, respectively. Further, the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted. At a 20 L fermentor, Lycogen⢠yields under the optimal culture condition are over 2 times than in the shake flask. The present results provide the Lycogen⢠optimal culture mediums, scale-up procedures and efficient extractions from R. sphaeroides WL-APD911. Keywords: Rhodobacter sphaeroides WL-APD911, Lycogenâ¢, Response surface methodology (RSM), Ferementation |
| format | Article |
| id | doaj-art-a7531f1df8424cccadd2313bf5532177 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-a7531f1df8424cccadd2313bf55321772025-02-03T06:49:31ZengTsinghua University PressFood Science and Human Wellness2213-45302017-12-0164195201A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategyCheng-Chin Wang0Shi-Ying Huang1Shu-Hung Huang2Zhi-Hong Wen3Jyun-Ying Huang4Wen-Sheng Liu5Hui-Min David Wang6Research and Development Department, Chia-Yi Steel, Tainan 717, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou 362000, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaDivision of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaDoctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan, China; Marine Biomedical Laboratory and Center for Translational Biopharmaceuticals, Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaGraduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ChinaDepartment and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, China; Asia-Pacific Biotech Developing, Inc. Kaohsiung 803, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Corresponding authors.Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan, China; Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, China; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan, China; Corresponding authors.The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The results reveal that a higher rotational speed exhibit a higher cell density, and the increasing in the amount of inoculum amount show a slight augment on the growth of R. sphaeroides WL-APD911.In the case of nitrogen sources adding, Lycogen⢠production was achieved with a 0.5 mM l-lysine supplementation. Moreover, the attention of Tween 80 presented a tremendous increase in the secondary metabolite. Response surface methodology (RSM) exhibited the optimization of medium supplements for Lycogen⢠invention is accomplished at molasses concentration of 10 g/L, yeast extract concentration of 40 g/L, 0.3% Tween 80 and NaCl concentration of 5 g/L, respectively. Further, the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted. At a 20 L fermentor, Lycogen⢠yields under the optimal culture condition are over 2 times than in the shake flask. The present results provide the Lycogen⢠optimal culture mediums, scale-up procedures and efficient extractions from R. sphaeroides WL-APD911. Keywords: Rhodobacter sphaeroides WL-APD911, Lycogenâ¢, Response surface methodology (RSM), Ferementationhttp://www.sciencedirect.com/science/article/pii/S2213453017301027 |
| spellingShingle | Cheng-Chin Wang Shi-Ying Huang Shu-Hung Huang Zhi-Hong Wen Jyun-Ying Huang Wen-Sheng Liu Hui-Min David Wang A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy Food Science and Human Wellness |
| title | A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy |
| title_full | A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy |
| title_fullStr | A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy |
| title_full_unstemmed | A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy |
| title_short | A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy |
| title_sort | synthetic biological secondary metabolite lycogena¢ produced and extracted from rhodobacter sphaeroides wl apd911 in an optimizatioal scale up strategy |
| url | http://www.sciencedirect.com/science/article/pii/S2213453017301027 |
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