A synthetic biological secondary metabolite, Lycogenâ¢, produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy
The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The resu...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2017-12-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453017301027 |
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| Summary: | The optimization of fermentation medium is important for synthetic biological secondary metabolite productions. The effect of rotation speed, inoculum amount, and medium supplements on the cell growth and Lycogen⢠secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated. The results reveal that a higher rotational speed exhibit a higher cell density, and the increasing in the amount of inoculum amount show a slight augment on the growth of R. sphaeroides WL-APD911.In the case of nitrogen sources adding, Lycogen⢠production was achieved with a 0.5 mM l-lysine supplementation. Moreover, the attention of Tween 80 presented a tremendous increase in the secondary metabolite. Response surface methodology (RSM) exhibited the optimization of medium supplements for Lycogen⢠invention is accomplished at molasses concentration of 10 g/L, yeast extract concentration of 40 g/L, 0.3% Tween 80 and NaCl concentration of 5 g/L, respectively. Further, the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted. At a 20 L fermentor, Lycogen⢠yields under the optimal culture condition are over 2 times than in the shake flask. The present results provide the Lycogen⢠optimal culture mediums, scale-up procedures and efficient extractions from R. sphaeroides WL-APD911. Keywords: Rhodobacter sphaeroides WL-APD911, Lycogenâ¢, Response surface methodology (RSM), Ferementation |
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| ISSN: | 2213-4530 |