Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production
As the global population continues to grow, the demand for sustainable food production methods becomes increasingly critical. This study investigates the presence of chromosomal abnormalities in bovine embryonic stem cells (bESCs), which hold potential for innovative food sources such as cultured me...
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Elsevier
2025-06-01
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| Series: | Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666833525000188 |
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| author | Chloë Deelkens Elly De Vlieghere Mario Van Poucke Masaki Kinoshita Jeffrey Aalders Lieven Thorrez Bert Devriendt Ann Van Soom Luc Peelman Björn Menten Catharina De Schauwer Jolanda van Hengel |
| author_facet | Chloë Deelkens Elly De Vlieghere Mario Van Poucke Masaki Kinoshita Jeffrey Aalders Lieven Thorrez Bert Devriendt Ann Van Soom Luc Peelman Björn Menten Catharina De Schauwer Jolanda van Hengel |
| author_sort | Chloë Deelkens |
| collection | DOAJ |
| description | As the global population continues to grow, the demand for sustainable food production methods becomes increasingly critical. This study investigates the presence of chromosomal abnormalities in bovine embryonic stem cells (bESCs), which hold potential for innovative food sources such as cultured meat. We derived three bESC lines from day-eight post-insemination blastocysts using a whole blastocyst plating approach. These cells maintained core pluripotency markers (POU5F1, SOX2, SALL4, NANOG) and demonstrated the ability to differentiate into the three germ layers, indicating their potential for sustainable food applications. Through shallow whole-genome sequencing, we identified various chromosomal anomalies, including mono-, tri-, and tetrasomies, with specific gains in chromosomes 7, 12, 27, and 29, and losses in chromosome 9. Notably, these aneuploidies progressively accumulated over time, raising concerns about genomic stability in long-term cultures. The implications of these genomic variants are significant for the development of cultured meat, as they may affect the efficiency and safety of production processes. This research underscores the necessity for systematic monitoring and optimizing culture conditions to mitigate genomic instabilities, ensuring the safe application of bESCs in sustainable food systems. Our findings pave the way for future innovations in clean food processing and utilising new food ingredients. |
| format | Article |
| id | doaj-art-a74e44528f794b95a8ea09b022e986e4 |
| institution | Kabale University |
| issn | 2666-8335 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Future Foods |
| spelling | doaj-art-a74e44528f794b95a8ea09b022e986e42025-02-05T04:32:45ZengElsevierFuture Foods2666-83352025-06-0111100555Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat productionChloë Deelkens0Elly De Vlieghere1Mario Van Poucke2Masaki Kinoshita3Jeffrey Aalders4Lieven Thorrez5Bert Devriendt6Ann Van Soom7Luc Peelman8Björn Menten9Catharina De Schauwer10Jolanda van Hengel11Medical Cell Biology Research Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, Corneel Heymanslaan 10 9000 Ghent, Belgium; Tissue Engineering Research Group, Department of Development and Regeneration, Faculty of Medicine, KU Leuven Kulak, Etienne Sabbelaan 53 8500 Kortrijk, Belgium; Laboratory of Immunology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133 9820 Merelbeke, BelgiumTissue Engineering Research Group, Department of Development and Regeneration, Faculty of Medicine, KU Leuven Kulak, Etienne Sabbelaan 53 8500 Kortrijk, Belgium; Polymer Chemistry and Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Krijgslaan 281 9000 Ghent, BelgiumLaboratory of Animal Genetics, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, BelgiumSchool of Biosciences, Faculty of Science, University of Nottingham, Sutton Bonington Leicestershire LE12 5RD, UKMedical Cell Biology Research Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, Corneel Heymanslaan 10 9000 Ghent, BelgiumTissue Engineering Research Group, Department of Development and Regeneration, Faculty of Medicine, KU Leuven Kulak, Etienne Sabbelaan 53 8500 Kortrijk, BelgiumLaboratory of Immunology, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133 9820 Merelbeke, BelgiumReproductive Biology Unit, Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133 9820 Merelbeke, BelgiumLaboratory of Animal Genetics, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, BelgiumCenter for Medical Genetics Ghent (CMGG), Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Corneel Heymanslaan 10 9000 Ghent, BelgiumVeterinary Stem Cell Research Unit, Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133 9820 Merelbeke, BelgiumMedical Cell Biology Research Group, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, Corneel Heymanslaan 10 9000 Ghent, Belgium; Corresponding author.As the global population continues to grow, the demand for sustainable food production methods becomes increasingly critical. This study investigates the presence of chromosomal abnormalities in bovine embryonic stem cells (bESCs), which hold potential for innovative food sources such as cultured meat. We derived three bESC lines from day-eight post-insemination blastocysts using a whole blastocyst plating approach. These cells maintained core pluripotency markers (POU5F1, SOX2, SALL4, NANOG) and demonstrated the ability to differentiate into the three germ layers, indicating their potential for sustainable food applications. Through shallow whole-genome sequencing, we identified various chromosomal anomalies, including mono-, tri-, and tetrasomies, with specific gains in chromosomes 7, 12, 27, and 29, and losses in chromosome 9. Notably, these aneuploidies progressively accumulated over time, raising concerns about genomic stability in long-term cultures. The implications of these genomic variants are significant for the development of cultured meat, as they may affect the efficiency and safety of production processes. This research underscores the necessity for systematic monitoring and optimizing culture conditions to mitigate genomic instabilities, ensuring the safe application of bESCs in sustainable food systems. Our findings pave the way for future innovations in clean food processing and utilising new food ingredients.http://www.sciencedirect.com/science/article/pii/S2666833525000188Bovine embryonic stem cellsAneuploidyCellular agricultureGenomic instabilityFood safetyCultured meat |
| spellingShingle | Chloë Deelkens Elly De Vlieghere Mario Van Poucke Masaki Kinoshita Jeffrey Aalders Lieven Thorrez Bert Devriendt Ann Van Soom Luc Peelman Björn Menten Catharina De Schauwer Jolanda van Hengel Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production Future Foods Bovine embryonic stem cells Aneuploidy Cellular agriculture Genomic instability Food safety Cultured meat |
| title | Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production |
| title_full | Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production |
| title_fullStr | Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production |
| title_full_unstemmed | Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production |
| title_short | Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production |
| title_sort | exploring aneuploidies in two center isolated bovine embryonic stem cell lines implications for cultured meat production |
| topic | Bovine embryonic stem cells Aneuploidy Cellular agriculture Genomic instability Food safety Cultured meat |
| url | http://www.sciencedirect.com/science/article/pii/S2666833525000188 |
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