Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L.
In the current study, an acidic polysaccharide from the in vitro suspension culture of Fumaria officinalis L. was obtained by extraction with 0.8% (w/v) aqueous ammonium oxalate. The polysaccharide fraction mainly consisted of galacturonic acid (41.0%), followed by galactose (7.3%) and arabinose (5....
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2018/5705036 |
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| author | Manol Ognyanov Yordan Georgiev Nadezhda Petkova Ivan Ivanov Ivelina Vasileva Maria Kratchanova |
| author_facet | Manol Ognyanov Yordan Georgiev Nadezhda Petkova Ivan Ivanov Ivelina Vasileva Maria Kratchanova |
| author_sort | Manol Ognyanov |
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| description | In the current study, an acidic polysaccharide from the in vitro suspension culture of Fumaria officinalis L. was obtained by extraction with 0.8% (w/v) aqueous ammonium oxalate. The polysaccharide fraction mainly consisted of galacturonic acid (41.0%), followed by galactose (7.3%) and arabinose (5.6%). This suggests the presence of arabinogalactan side chains in the rhamnogalacturonan-I segment of the studied pectin, which was mainly built up by homogalacturonan segments. The pectin was evaluated as low-methyl-esterified (45.0%) with degree of acetylation 3.4%. The polymer fraction was consisted of different molecular weight populations in the range of 6–600 kDa. The high amount of 4-L-hydroxyproline (11.7% of total protein) and the specific positive reaction to Yariv’s phenylglycoside reagent indicated the presence of an arabinogalactan protein in the cell walls. The functional properties of the polysaccharide fraction were evaluated, as it possessed better water-holding capacity than oil-holding capacity. The studied pectin demonstrated significant foaming ability and promising emulsifying properties in a concentration 1%. Therefore, the isolated polysaccharide fraction could be successfully used as emulsifier and foaming agent in food products and pharmaceutical supplements. |
| format | Article |
| id | doaj-art-a7169e0607a34b938e78a5dcdc615312 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | International Journal of Polymer Science |
| spelling | doaj-art-a7169e0607a34b938e78a5dcdc6153122025-02-03T01:24:14ZengWileyInternational Journal of Polymer Science1687-94221687-94302018-01-01201810.1155/2018/57050365705036Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L.Manol Ognyanov0Yordan Georgiev1Nadezhda Petkova2Ivan Ivanov3Ivelina Vasileva4Maria Kratchanova5Laboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, BulgariaLaboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, BulgariaDepartment of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, Technological Faculty, 26 Maritza Blvd, 4002 Plovdiv, BulgariaDepartment of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, Technological Faculty, 26 Maritza Blvd, 4002 Plovdiv, BulgariaDepartment of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, Technological Faculty, 26 Maritza Blvd, 4002 Plovdiv, BulgariaLaboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, BulgariaIn the current study, an acidic polysaccharide from the in vitro suspension culture of Fumaria officinalis L. was obtained by extraction with 0.8% (w/v) aqueous ammonium oxalate. The polysaccharide fraction mainly consisted of galacturonic acid (41.0%), followed by galactose (7.3%) and arabinose (5.6%). This suggests the presence of arabinogalactan side chains in the rhamnogalacturonan-I segment of the studied pectin, which was mainly built up by homogalacturonan segments. The pectin was evaluated as low-methyl-esterified (45.0%) with degree of acetylation 3.4%. The polymer fraction was consisted of different molecular weight populations in the range of 6–600 kDa. The high amount of 4-L-hydroxyproline (11.7% of total protein) and the specific positive reaction to Yariv’s phenylglycoside reagent indicated the presence of an arabinogalactan protein in the cell walls. The functional properties of the polysaccharide fraction were evaluated, as it possessed better water-holding capacity than oil-holding capacity. The studied pectin demonstrated significant foaming ability and promising emulsifying properties in a concentration 1%. Therefore, the isolated polysaccharide fraction could be successfully used as emulsifier and foaming agent in food products and pharmaceutical supplements.http://dx.doi.org/10.1155/2018/5705036 |
| spellingShingle | Manol Ognyanov Yordan Georgiev Nadezhda Petkova Ivan Ivanov Ivelina Vasileva Maria Kratchanova Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L. International Journal of Polymer Science |
| title | Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L. |
| title_full | Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L. |
| title_fullStr | Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L. |
| title_full_unstemmed | Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L. |
| title_short | Isolation and Characterization of Pectic Polysaccharide Fraction from In Vitro Suspension Culture of Fumaria officinalis L. |
| title_sort | isolation and characterization of pectic polysaccharide fraction from in vitro suspension culture of fumaria officinalis l |
| url | http://dx.doi.org/10.1155/2018/5705036 |
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