Zinc mediates the neuronal activity-dependent anti-apoptotic effect.
Synaptic activity increases the resistance of neurons to diverse apoptotic insults; however, the underlying mechanisms remain less well understood. Zinc promotes cell survival under varied conditions, but the role of synaptically released zinc in the activity-dependent anti-apoptotic effect is unkno...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182150&type=printable |
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| author | Mei Qiu Yang-Ping Shentu Ji Zeng Xiao-Chuan Wang Xiong Yan Xin-Wen Zhou Xiao-Peng Jing Qun Wang Heng-Ye Man Jian-Zhi Wang Rong Liu |
| author_facet | Mei Qiu Yang-Ping Shentu Ji Zeng Xiao-Chuan Wang Xiong Yan Xin-Wen Zhou Xiao-Peng Jing Qun Wang Heng-Ye Man Jian-Zhi Wang Rong Liu |
| author_sort | Mei Qiu |
| collection | DOAJ |
| description | Synaptic activity increases the resistance of neurons to diverse apoptotic insults; however, the underlying mechanisms remain less well understood. Zinc promotes cell survival under varied conditions, but the role of synaptically released zinc in the activity-dependent anti-apoptotic effect is unknown. Using cultured hippocampal slices and primary neurons we show that a typical apoptosis inducer-staurosporine (STP) was able to cause concentration-dependent apoptotic cell death in brain slices; Enhanced synaptic activity by bicuculline (Bic)/4-Aminopyridine (AP) treatment effectively prevented neurons from STP-induced cell apoptosis, as indicated by increased cell survival and suppressed caspase-3 activity. Application of Ca-EDTA, a cell membrane-impermeable zinc chelator which can efficiently capture the synaptically released zinc, completely blocked the neuronal activity-dependent anti-apoptotic effect. Same results were also observed in cultured primary hippocampal neurons. Therefore, our results indicate that synaptic activity improves neuronal resistance to apoptosis via synaptically released zinc. |
| format | Article |
| id | doaj-art-a70fdd1b711347c98714eae87d0a960d |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a70fdd1b711347c98714eae87d0a960d2025-08-20T02:03:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018215010.1371/journal.pone.0182150Zinc mediates the neuronal activity-dependent anti-apoptotic effect.Mei QiuYang-Ping ShentuJi ZengXiao-Chuan WangXiong YanXin-Wen ZhouXiao-Peng JingQun WangHeng-Ye ManJian-Zhi WangRong LiuSynaptic activity increases the resistance of neurons to diverse apoptotic insults; however, the underlying mechanisms remain less well understood. Zinc promotes cell survival under varied conditions, but the role of synaptically released zinc in the activity-dependent anti-apoptotic effect is unknown. Using cultured hippocampal slices and primary neurons we show that a typical apoptosis inducer-staurosporine (STP) was able to cause concentration-dependent apoptotic cell death in brain slices; Enhanced synaptic activity by bicuculline (Bic)/4-Aminopyridine (AP) treatment effectively prevented neurons from STP-induced cell apoptosis, as indicated by increased cell survival and suppressed caspase-3 activity. Application of Ca-EDTA, a cell membrane-impermeable zinc chelator which can efficiently capture the synaptically released zinc, completely blocked the neuronal activity-dependent anti-apoptotic effect. Same results were also observed in cultured primary hippocampal neurons. Therefore, our results indicate that synaptic activity improves neuronal resistance to apoptosis via synaptically released zinc.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182150&type=printable |
| spellingShingle | Mei Qiu Yang-Ping Shentu Ji Zeng Xiao-Chuan Wang Xiong Yan Xin-Wen Zhou Xiao-Peng Jing Qun Wang Heng-Ye Man Jian-Zhi Wang Rong Liu Zinc mediates the neuronal activity-dependent anti-apoptotic effect. PLoS ONE |
| title | Zinc mediates the neuronal activity-dependent anti-apoptotic effect. |
| title_full | Zinc mediates the neuronal activity-dependent anti-apoptotic effect. |
| title_fullStr | Zinc mediates the neuronal activity-dependent anti-apoptotic effect. |
| title_full_unstemmed | Zinc mediates the neuronal activity-dependent anti-apoptotic effect. |
| title_short | Zinc mediates the neuronal activity-dependent anti-apoptotic effect. |
| title_sort | zinc mediates the neuronal activity dependent anti apoptotic effect |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182150&type=printable |
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