New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response
Bt has been applied as a gene source for insect-resistant transgenic crops, which represents efficient control of insect pests. In this study, we evaluated the pesticidal specificity of one Bt maize strain, DBN9936, that expresses Cry1Ab protein in <i>Spodoptera litura</i> larvae. The re...
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MDPI AG
2024-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/16/1/10 |
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| author | Yan-Jue Wang Ya-Qin Shen Ying-Dan Xiao Xue Yang Shao-Peng Hao Jian-Qiu Liu Xiao-Xue Yang Kazuei Mita Ya-Jing Xu |
| author_facet | Yan-Jue Wang Ya-Qin Shen Ying-Dan Xiao Xue Yang Shao-Peng Hao Jian-Qiu Liu Xiao-Xue Yang Kazuei Mita Ya-Jing Xu |
| author_sort | Yan-Jue Wang |
| collection | DOAJ |
| description | Bt has been applied as a gene source for insect-resistant transgenic crops, which represents efficient control of insect pests. In this study, we evaluated the pesticidal specificity of one Bt maize strain, DBN9936, that expresses Cry1Ab protein in <i>Spodoptera litura</i> larvae. The results showed that this Bt maize is active against the younger larvae while causing a sublethal effect on older larvae. To further assess the biological responses of <i>S. litura</i> under sublethal Cry1Ab exposure, the relative concentrations of Cry1Ab in different tissues of fifth instar larvae were investigated, indicating that Cry1Ab is transported along the gut and out of the body via excretion or into the hemocoel and lead to sequestration by molting. Furthermore, the result of ultrastructural observation in sublethal Cry1Ab-treated midgut, namely an increase in lysosome number, with the lysosomal activity activation simultaneously provides a strong indication that lysosome plays an active role in response to sublethal Cry1Ab exposure. |
| format | Article |
| id | doaj-art-a6fe9730db7542e3a6b2480df399b4aa |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-a6fe9730db7542e3a6b2480df399b4aa2025-01-24T13:35:34ZengMDPI AGInsects2075-44502024-12-011611010.3390/insects16010010New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal ResponseYan-Jue Wang0Ya-Qin Shen1Ying-Dan Xiao2Xue Yang3Shao-Peng Hao4Jian-Qiu Liu5Xiao-Xue Yang6Kazuei Mita7Ya-Jing Xu8Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, ChinaBt has been applied as a gene source for insect-resistant transgenic crops, which represents efficient control of insect pests. In this study, we evaluated the pesticidal specificity of one Bt maize strain, DBN9936, that expresses Cry1Ab protein in <i>Spodoptera litura</i> larvae. The results showed that this Bt maize is active against the younger larvae while causing a sublethal effect on older larvae. To further assess the biological responses of <i>S. litura</i> under sublethal Cry1Ab exposure, the relative concentrations of Cry1Ab in different tissues of fifth instar larvae were investigated, indicating that Cry1Ab is transported along the gut and out of the body via excretion or into the hemocoel and lead to sequestration by molting. Furthermore, the result of ultrastructural observation in sublethal Cry1Ab-treated midgut, namely an increase in lysosome number, with the lysosomal activity activation simultaneously provides a strong indication that lysosome plays an active role in response to sublethal Cry1Ab exposure.https://www.mdpi.com/2075-4450/16/1/10<i>S. litura</i>Bt-Cry1Absublethal effecttissular distributionTEMlysosome |
| spellingShingle | Yan-Jue Wang Ya-Qin Shen Ying-Dan Xiao Xue Yang Shao-Peng Hao Jian-Qiu Liu Xiao-Xue Yang Kazuei Mita Ya-Jing Xu New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response Insects <i>S. litura</i> Bt-Cry1Ab sublethal effect tissular distribution TEM lysosome |
| title | New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response |
| title_full | New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response |
| title_fullStr | New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response |
| title_full_unstemmed | New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response |
| title_short | New Insight on the Sublethal Effect of Bt-Cry1Ab in <i>Spodoptera litura</i> (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response |
| title_sort | new insight on the sublethal effect of bt cry1ab in i spodoptera litura i fabricius tissular distribution of cry1ab ultrastructural alterations and the lysosomal response |
| topic | <i>S. litura</i> Bt-Cry1Ab sublethal effect tissular distribution TEM lysosome |
| url | https://www.mdpi.com/2075-4450/16/1/10 |
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