Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i>
Palm oil, widely used in various products, poses environmental and climate change risks. “Yeast oil” produced by <i>Lipomyces starkeyi</i>, an oil-producing yeast, is one of the sustainable alternatives for palm oil and was successfully produced as an edible substitute for palm oil. Howe...
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MDPI AG
2024-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/1/7 |
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| author | Hiroya Taki Kentaro Mine Mana Miyamoto Jiro Seto Shinji Matsuo Kazuo Kumagai Hideto Matsuyama |
| author_facet | Hiroya Taki Kentaro Mine Mana Miyamoto Jiro Seto Shinji Matsuo Kazuo Kumagai Hideto Matsuyama |
| author_sort | Hiroya Taki |
| collection | DOAJ |
| description | Palm oil, widely used in various products, poses environmental and climate change risks. “Yeast oil” produced by <i>Lipomyces starkeyi</i>, an oil-producing yeast, is one of the sustainable alternatives for palm oil and was successfully produced as an edible substitute for palm oil. However, the high cost of the culture medium for oil production remains a challenge for practical applications. Okara is a by-product of tofu and soymilk production. Because yeast extract contributes to the high cost of the culture medium, we considered using okara, a cheap and nitrogen-rich substitute, to reduce costs. In the initial study with okara, the production of yeast oil was confirmed, but its productivity was low due to the high viscosity caused by its insoluble solids. To overcome this, we extracted and concentrated nitrogen components in okara using the membrane concentration process. Using NF (nanofiltration) membrane concentration, oil production increased 1.69 and 1.44 times compared to the unconcentrated extract solution (added 90% (v/v) in the culture medium) and yeast extract (added 5% (w/v) in the culture medium), respectively. These findings indicate the potential for a significant cost reduction in the culture medium and high oil yield in yeast oil production. |
| format | Article |
| id | doaj-art-a6f3a8842e5f4e31b1147a6b163e5072 |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-a6f3a8842e5f4e31b1147a6b163e50722025-01-24T13:32:01ZengMDPI AGFermentation2311-56372024-12-01111710.3390/fermentation11010007Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i>Hiroya Taki0Kentaro Mine1Mana Miyamoto2Jiro Seto3Shinji Matsuo4Kazuo Kumagai5Hideto Matsuyama6Department of Chemical Science and Engineering, Kobe University, Kobe 657-8501, JapanNissin Global Innovation Center, Nissin Foods Holdings Co., Ltd., Hachioji 192-0001, JapanNissin Global Innovation Center, Nissin Foods Holdings Co., Ltd., Hachioji 192-0001, JapanNissin Global Innovation Center, Nissin Foods Holdings Co., Ltd., Hachioji 192-0001, JapanNissin Global Innovation Center, Nissin Foods Holdings Co., Ltd., Hachioji 192-0001, JapanResearch Center for Membrane and Film Technology, Kobe University, Kobe 657-8501, JapanDepartment of Chemical Science and Engineering, Kobe University, Kobe 657-8501, JapanPalm oil, widely used in various products, poses environmental and climate change risks. “Yeast oil” produced by <i>Lipomyces starkeyi</i>, an oil-producing yeast, is one of the sustainable alternatives for palm oil and was successfully produced as an edible substitute for palm oil. However, the high cost of the culture medium for oil production remains a challenge for practical applications. Okara is a by-product of tofu and soymilk production. Because yeast extract contributes to the high cost of the culture medium, we considered using okara, a cheap and nitrogen-rich substitute, to reduce costs. In the initial study with okara, the production of yeast oil was confirmed, but its productivity was low due to the high viscosity caused by its insoluble solids. To overcome this, we extracted and concentrated nitrogen components in okara using the membrane concentration process. Using NF (nanofiltration) membrane concentration, oil production increased 1.69 and 1.44 times compared to the unconcentrated extract solution (added 90% (v/v) in the culture medium) and yeast extract (added 5% (w/v) in the culture medium), respectively. These findings indicate the potential for a significant cost reduction in the culture medium and high oil yield in yeast oil production.https://www.mdpi.com/2311-5637/11/1/7oil-producing microorganism<i>Lipomyces starkeyi</i>lipidenvironmentally friendlybio-oilalternative palm oil |
| spellingShingle | Hiroya Taki Kentaro Mine Mana Miyamoto Jiro Seto Shinji Matsuo Kazuo Kumagai Hideto Matsuyama Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i> Fermentation oil-producing microorganism <i>Lipomyces starkeyi</i> lipid environmentally friendly bio-oil alternative palm oil |
| title | Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i> |
| title_full | Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i> |
| title_fullStr | Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i> |
| title_full_unstemmed | Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i> |
| title_short | Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, <i>Lipomyces starkeyi</i> |
| title_sort | utilization of okara as a culture medium by membrane concentration process for high oil production by oleaginous yeast i lipomyces starkeyi i |
| topic | oil-producing microorganism <i>Lipomyces starkeyi</i> lipid environmentally friendly bio-oil alternative palm oil |
| url | https://www.mdpi.com/2311-5637/11/1/7 |
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