Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate
In our previous studies we showed antitumor and anti-inflammatory activities of protein kinases inhibitor pyrrol derivate 1-(4-Cl-benzyl)-3-Cl-4-(CF3-fenylamino)-1H-pyrrol-2,5-dione (MI-1) on rat colon cancer model. Therefore anti-inflammatory effect of MI-1 on rat acetic acid induced ulcerative col...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/2145753 |
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| author | Halyna M. Kuznietsova Maryna S. Yena Iryna P. Kotlyar Olexandr V. Ogloblya Volodymyr K. Rybalchenko |
| author_facet | Halyna M. Kuznietsova Maryna S. Yena Iryna P. Kotlyar Olexandr V. Ogloblya Volodymyr K. Rybalchenko |
| author_sort | Halyna M. Kuznietsova |
| collection | DOAJ |
| description | In our previous studies we showed antitumor and anti-inflammatory activities of protein kinases inhibitor pyrrol derivate 1-(4-Cl-benzyl)-3-Cl-4-(CF3-fenylamino)-1H-pyrrol-2,5-dione (MI-1) on rat colon cancer model. Therefore anti-inflammatory effect of MI-1 on rat acetic acid induced ulcerative colitis (UC) model was aimed to be discovered. The anti-inflammatory effects of MI-1 (2.7 mg/kg daily) compared to reference drug Prednisolone (0.7 mg/kg daily) after 14-day usage were evaluated on macro- and light microscopy levels and expressed in 21-grade scale. Redox status of bowel mucosa was also estimated. It was shown that in UC group the grade of total injury (GTI) was equal to 9.6 (GTIcontrol=0). Increase of malonic dialdehyde (MDA) by 89% and protein carbonyl groups (PCG) by 60% and decrease of superoxide dismutase (SOD) by 40% were also observed. Prednisolone decreased GTI to 3 and leveled SOD activity, but MDA and PCG remained higher than control ones by 52% and 42%, respectively. MI-1 restored colon mucosa integrity and decreased mucosa inflammation down to GTI = 0.5 and leveled PCG and SOD. Thus, MI-1 possessed anti-inflammatory properties, which were more expressed that Prednisolone ones, as well as normalized mucosa redox balance, and so has a prospect for correction of inflammatory processes. |
| format | Article |
| id | doaj-art-a6e4edb23a4a4d20b8e0ad540703b221 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a6e4edb23a4a4d20b8e0ad540703b2212025-02-03T05:51:49ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/21457532145753Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol DerivateHalyna M. Kuznietsova0Maryna S. Yena1Iryna P. Kotlyar2Olexandr V. Ogloblya3Volodymyr K. Rybalchenko4Institute of Biology, Taras Shevchenko National University, Volodymyrska 64/13, Kyiv 01601, UkraineInstitute of Biology, Taras Shevchenko National University, Volodymyrska 64/13, Kyiv 01601, UkraineInstitute of Biology, Taras Shevchenko National University, Volodymyrska 64/13, Kyiv 01601, UkraineInstitute of Biology, Taras Shevchenko National University, Volodymyrska 64/13, Kyiv 01601, UkraineInstitute of Biology, Taras Shevchenko National University, Volodymyrska 64/13, Kyiv 01601, UkraineIn our previous studies we showed antitumor and anti-inflammatory activities of protein kinases inhibitor pyrrol derivate 1-(4-Cl-benzyl)-3-Cl-4-(CF3-fenylamino)-1H-pyrrol-2,5-dione (MI-1) on rat colon cancer model. Therefore anti-inflammatory effect of MI-1 on rat acetic acid induced ulcerative colitis (UC) model was aimed to be discovered. The anti-inflammatory effects of MI-1 (2.7 mg/kg daily) compared to reference drug Prednisolone (0.7 mg/kg daily) after 14-day usage were evaluated on macro- and light microscopy levels and expressed in 21-grade scale. Redox status of bowel mucosa was also estimated. It was shown that in UC group the grade of total injury (GTI) was equal to 9.6 (GTIcontrol=0). Increase of malonic dialdehyde (MDA) by 89% and protein carbonyl groups (PCG) by 60% and decrease of superoxide dismutase (SOD) by 40% were also observed. Prednisolone decreased GTI to 3 and leveled SOD activity, but MDA and PCG remained higher than control ones by 52% and 42%, respectively. MI-1 restored colon mucosa integrity and decreased mucosa inflammation down to GTI = 0.5 and leveled PCG and SOD. Thus, MI-1 possessed anti-inflammatory properties, which were more expressed that Prednisolone ones, as well as normalized mucosa redox balance, and so has a prospect for correction of inflammatory processes.http://dx.doi.org/10.1155/2016/2145753 |
| spellingShingle | Halyna M. Kuznietsova Maryna S. Yena Iryna P. Kotlyar Olexandr V. Ogloblya Volodymyr K. Rybalchenko Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate The Scientific World Journal |
| title | Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate |
| title_full | Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate |
| title_fullStr | Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate |
| title_full_unstemmed | Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate |
| title_short | Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate |
| title_sort | anti inflammatory effects of protein kinase inhibitor pyrrol derivate |
| url | http://dx.doi.org/10.1155/2016/2145753 |
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