Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complicated genetic inheritance. Programmed death 1 (PD-1), a negative T cell regulator to maintain peripheral tolerance, induces negative signals to T cells during interaction with its ligands and is therefore a candidate g...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2014/950903 |
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| author | Qingqing Jiao Cuiping Liu Ziliang Yang Qiang Ding Miaomiao Wang Min Li Tingting Zhu Hua Qian Wei Li Na Tu Fumin Fang Licai Ye Zuotao Zhao Qihong Qian |
| author_facet | Qingqing Jiao Cuiping Liu Ziliang Yang Qiang Ding Miaomiao Wang Min Li Tingting Zhu Hua Qian Wei Li Na Tu Fumin Fang Licai Ye Zuotao Zhao Qihong Qian |
| author_sort | Qingqing Jiao |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complicated genetic inheritance. Programmed death 1 (PD-1), a negative T cell regulator to maintain peripheral tolerance, induces negative signals to T cells during interaction with its ligands and is therefore a candidate gene in the development of SLE. In order to examine whether expression levels of PD-1 contribute to the pathogenesis of SLE, 30 patients with SLE and 30 controls were recruited and their PD-1 expression levels in peripheral blood mononuclear cells (PBMCs) were measured via flow cytometry and quantitative real-time-reverse transcription polymerase chain reaction (RT-PCR). Also, whether PD-1 expression levels are associated with the variant of the SNP rs36084323 and the SLE Disease Activity Index (SLEDAI) was studied in this work. The PD-1 expression levels of SLE patients were significantly increased compared with those of the healthy controls. The upregulated PD-1 expression levels in SLE patients were greatly associated with SLEDAI scores. No significant difference was found between PD-1 expression levels and SNP rs36084323. The results suggest that increased expression of PD-1 may correlate with the pathogenesis of SLE, upregulated PD-1 expression may be a biomarker for SLE diagnosis, and PD-1 inhibitor may be useful to SLE treatment. |
| format | Article |
| id | doaj-art-a6e3d02aab2f49e8bd9db6b62296b87b |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-a6e3d02aab2f49e8bd9db6b62296b87b2025-08-20T02:20:10ZengWileyInternational Journal of Genomics2314-436X2314-43782014-01-01201410.1155/2014/950903950903Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 GeneQingqing Jiao0Cuiping Liu1Ziliang Yang2Qiang Ding3Miaomiao Wang4Min Li5Tingting Zhu6Hua Qian7Wei Li8Na Tu9Fumin Fang10Licai Ye11Zuotao Zhao12Qihong Qian13Department of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaClinical Immunology Laboratory, First Affiliated Hospital, Soochow University, 708 Renmin Road, Suzhou 215007, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, Soochow University Affiliated Children’s Hospital, 303 Jingde Road, Suzhou 215003, ChinaDepartment of Dermatology, Soochow University Affiliated Children’s Hospital, 303 Jingde Road, Suzhou 215003, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaDepartment of Dermatology, First Hospital, Peking University, 8 Xishenku Road, Beijing 100034, ChinaDepartment of Dermatology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, ChinaSystemic lupus erythematosus (SLE) is a multisystem autoimmune disease with complicated genetic inheritance. Programmed death 1 (PD-1), a negative T cell regulator to maintain peripheral tolerance, induces negative signals to T cells during interaction with its ligands and is therefore a candidate gene in the development of SLE. In order to examine whether expression levels of PD-1 contribute to the pathogenesis of SLE, 30 patients with SLE and 30 controls were recruited and their PD-1 expression levels in peripheral blood mononuclear cells (PBMCs) were measured via flow cytometry and quantitative real-time-reverse transcription polymerase chain reaction (RT-PCR). Also, whether PD-1 expression levels are associated with the variant of the SNP rs36084323 and the SLE Disease Activity Index (SLEDAI) was studied in this work. The PD-1 expression levels of SLE patients were significantly increased compared with those of the healthy controls. The upregulated PD-1 expression levels in SLE patients were greatly associated with SLEDAI scores. No significant difference was found between PD-1 expression levels and SNP rs36084323. The results suggest that increased expression of PD-1 may correlate with the pathogenesis of SLE, upregulated PD-1 expression may be a biomarker for SLE diagnosis, and PD-1 inhibitor may be useful to SLE treatment.http://dx.doi.org/10.1155/2014/950903 |
| spellingShingle | Qingqing Jiao Cuiping Liu Ziliang Yang Qiang Ding Miaomiao Wang Min Li Tingting Zhu Hua Qian Wei Li Na Tu Fumin Fang Licai Ye Zuotao Zhao Qihong Qian Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene International Journal of Genomics |
| title | Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene |
| title_full | Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene |
| title_fullStr | Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene |
| title_full_unstemmed | Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene |
| title_short | Upregulated PD-1 Expression Is Associated with the Development of Systemic Lupus Erythematosus, but Not the PD-1.1 Allele of the PDCD1 Gene |
| title_sort | upregulated pd 1 expression is associated with the development of systemic lupus erythematosus but not the pd 1 1 allele of the pdcd1 gene |
| url | http://dx.doi.org/10.1155/2014/950903 |
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