H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
A new, simple, sensitive and selective H-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing...
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Format: | Article |
Language: | English |
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Wiley
2011-01-01
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Series: | E-Journal of Chemistry |
Online Access: | http://dx.doi.org/10.1155/2011/210259 |
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author | Amandeep Kaur Usha Gupta |
author_facet | Amandeep Kaur Usha Gupta |
author_sort | Amandeep Kaur |
collection | DOAJ |
description | A new, simple, sensitive and selective H-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing reagent at pH 5.5 and solubilizing complexes in triton x-100 micellar media. Absorbances at the two pairs of wavelengths, 540 and 550 nm (when eosin acts as analyte) or 518 and 542 nm (when erythrosine act as analyte) were monitored. This method has satisfactorily been applied for the determination of eosin and erythrosine dyes in synthetic mixtures and commercial products. |
format | Article |
id | doaj-art-a6c446d188094d5bbbc45b8acd704eb0 |
institution | Kabale University |
issn | 0973-4945 2090-9810 |
language | English |
publishDate | 2011-01-01 |
publisher | Wiley |
record_format | Article |
series | E-Journal of Chemistry |
spelling | doaj-art-a6c446d188094d5bbbc45b8acd704eb02025-02-03T05:46:10ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01841979198510.1155/2011/210259H-Point Standard Addition Method for Simultaneous Determination of Eosin and ErytrosineAmandeep Kaur0Usha Gupta1Department of Chemistry, Punjabi University, Patiala-147002, Punjab, IndiaDepartment of Chemistry, Punjabi University, Patiala-147002, Punjab, IndiaA new, simple, sensitive and selective H-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing reagent at pH 5.5 and solubilizing complexes in triton x-100 micellar media. Absorbances at the two pairs of wavelengths, 540 and 550 nm (when eosin acts as analyte) or 518 and 542 nm (when erythrosine act as analyte) were monitored. This method has satisfactorily been applied for the determination of eosin and erythrosine dyes in synthetic mixtures and commercial products.http://dx.doi.org/10.1155/2011/210259 |
spellingShingle | Amandeep Kaur Usha Gupta H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine E-Journal of Chemistry |
title | H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine |
title_full | H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine |
title_fullStr | H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine |
title_full_unstemmed | H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine |
title_short | H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine |
title_sort | h point standard addition method for simultaneous determination of eosin and erytrosine |
url | http://dx.doi.org/10.1155/2011/210259 |
work_keys_str_mv | AT amandeepkaur hpointstandardadditionmethodforsimultaneousdeterminationofeosinanderytrosine AT ushagupta hpointstandardadditionmethodforsimultaneousdeterminationofeosinanderytrosine |