Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil
Hydroxyl radicals are potent mutagens that attack DNA to form various base and ribose derivatives. One of the major damaged thymine derivatives is 5-formyluracil (fU), which induces pyrimidine transition during replication. In order to establish the structural basis for such mutagenesis, the crystal...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/107289 |
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| author | Masaru Tsunoda Takeshi Sakaue Satoko Naito Tomoko Sunami Naoko Abe Yoshihito Ueno Akira Matsuda Akio Takénaka |
| author_facet | Masaru Tsunoda Takeshi Sakaue Satoko Naito Tomoko Sunami Naoko Abe Yoshihito Ueno Akira Matsuda Akio Takénaka |
| author_sort | Masaru Tsunoda |
| collection | DOAJ |
| description | Hydroxyl radicals are potent mutagens that attack DNA to form various base and ribose derivatives. One of the major damaged thymine derivatives is 5-formyluracil (fU), which induces pyrimidine transition during replication. In order to establish the structural basis for such mutagenesis, the crystal structures of two kinds of DNA d(CGCGRATfUCGCG) with R = A/G have been determined by X-ray crystallography. The fU residues form a Watson-Crick-type pair with A and two types of pairs (wobble and reversed wobble) with G, the latter being a new type of base pair between ionized thymine base and guanine base. In silico structural modeling suggests that the DNA polymerase can accept the reversed wobble pair with G, as well as the Watson-Crick pair with A. |
| format | Article |
| id | doaj-art-a6c26a512f31499ca28e247e1e9392ca |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-a6c26a512f31499ca28e247e1e9392ca2025-02-03T01:02:00ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/107289107289Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-FormyluracilMasaru Tsunoda0Takeshi Sakaue1Satoko Naito2Tomoko Sunami3Naoko Abe4Yoshihito Ueno5Akira Matsuda6Akio Takénaka7Faculty of Pharmacy, Iwaki Meisei University, Chuodai-Iino, Iwaki 970-8551, JapanGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, JapanGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, JapanGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, JapanGraduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, JapanGraduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, JapanGraduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, JapanFaculty of Pharmacy, Iwaki Meisei University, Chuodai-Iino, Iwaki 970-8551, JapanHydroxyl radicals are potent mutagens that attack DNA to form various base and ribose derivatives. One of the major damaged thymine derivatives is 5-formyluracil (fU), which induces pyrimidine transition during replication. In order to establish the structural basis for such mutagenesis, the crystal structures of two kinds of DNA d(CGCGRATfUCGCG) with R = A/G have been determined by X-ray crystallography. The fU residues form a Watson-Crick-type pair with A and two types of pairs (wobble and reversed wobble) with G, the latter being a new type of base pair between ionized thymine base and guanine base. In silico structural modeling suggests that the DNA polymerase can accept the reversed wobble pair with G, as well as the Watson-Crick pair with A.http://dx.doi.org/10.4061/2010/107289 |
| spellingShingle | Masaru Tsunoda Takeshi Sakaue Satoko Naito Tomoko Sunami Naoko Abe Yoshihito Ueno Akira Matsuda Akio Takénaka Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil Journal of Nucleic Acids |
| title | Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil |
| title_full | Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil |
| title_fullStr | Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil |
| title_full_unstemmed | Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil |
| title_short | Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil |
| title_sort | insights into the structures of dna damaged by hydroxyl radical crystal structures of dna duplexes containing 5 formyluracil |
| url | http://dx.doi.org/10.4061/2010/107289 |
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