Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production
IntroductionThe purpose of this study was to investigate how in vitro gas production (GP) and ruminal fermentation characteristics were affected by increasing concentrations of green algae plant (C. reinhardtii) extracts in combination with nanoparticles MgO and MgS.MethodsA solution containing 0.1...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1492230/full |
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| author | Valiollah Palangi Adem Kaya Muhlis Macit Hayrunnisa Nadaroglu Hayrullah Bora Ünlü Ali Kaya Ashkan Fekri Ayaz Mammadov Maximilian Lackner |
| author_facet | Valiollah Palangi Adem Kaya Muhlis Macit Hayrunnisa Nadaroglu Hayrullah Bora Ünlü Ali Kaya Ashkan Fekri Ayaz Mammadov Maximilian Lackner |
| author_sort | Valiollah Palangi |
| collection | DOAJ |
| description | IntroductionThe purpose of this study was to investigate how in vitro gas production (GP) and ruminal fermentation characteristics were affected by increasing concentrations of green algae plant (C. reinhardtii) extracts in combination with nanoparticles MgO and MgS.MethodsA solution containing 0.1 M MgCl2 was prepared in 300 mL for the green production of MgCl nanoparticles. The mixture was refluxed for two hours at 85°C using a reflux condenser after 10 mL of pomegranate plant extract was added. The green algal plant (C. reinhardtii), which has many non-toxic antioxidants, was used as a carbon source to produce carbon quantum dots (CQD). Chemical analysis was conducted in accordance with AOAC (2005) recommendations. Rumen fluid from recently slaughtered calves is used to produce in vitro gas immediately following slaughter. Analysis of variance (ANOVA) was performed on the obtained data from the in vitro study in a completely randomized design using the mixed model of SAS (version 9.4; Inc., Cary NC, USA).Results and DiscussionThe variance analysis results and the average values of the chemical compositions were significantly influenced by the extracts (all p < 0.0001). In this line, the values of net gas, pH, OMD, ME, NEl, and ME were found to be the highest for Algae + 50 MgO and the lowest for Algae + 50 MgS, respectively (all p < 0.0001). These promising results imply that extracts from C. Reinhardtii may be able to mitigate the adverse consequences of rumen fermentation. To precisely ascertain the impact particular Rhodophyta on greenhouse gas emissions, additional investigation is needed. |
| format | Article |
| id | doaj-art-a6b39c076b824fc89c3b230d7c8e1234 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-a6b39c076b824fc89c3b230d7c8e12342025-02-03T13:48:52ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011210.3389/fvets.2025.14922301492230Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane productionValiollah Palangi0Adem Kaya1Muhlis Macit2Hayrunnisa Nadaroglu3Hayrullah Bora Ünlü4Ali Kaya5Ashkan Fekri6Ayaz Mammadov7Maximilian Lackner8Department of Animal Science, Faculty of Agriculture, Ege University, Izmir, TürkiyeDepartment of Animal Science, Faculty of Agriculture, Ataturk University, Erzurum, TürkiyeDepartment of Animal Science, Faculty of Agriculture, Ataturk University, Erzurum, TürkiyeDepartment of Nano-Science and Nano-Engineering, Institute of Science and Technology, Ataturk University, Erzurum, TürkiyeDepartment of Animal Science, Faculty of Agriculture, Ege University, Izmir, TürkiyeDepartment of Animal Science, Faculty of Agriculture, Ataturk University, Erzurum, TürkiyeDepartment of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Alborz, Karaj, IranDepartment of Life Sciences, Western Caspian University, Baku, AzerbaijanDepartment of Industrial Engineering, University of Applied Sciences Technikum Wien, Vienna, AustriaIntroductionThe purpose of this study was to investigate how in vitro gas production (GP) and ruminal fermentation characteristics were affected by increasing concentrations of green algae plant (C. reinhardtii) extracts in combination with nanoparticles MgO and MgS.MethodsA solution containing 0.1 M MgCl2 was prepared in 300 mL for the green production of MgCl nanoparticles. The mixture was refluxed for two hours at 85°C using a reflux condenser after 10 mL of pomegranate plant extract was added. The green algal plant (C. reinhardtii), which has many non-toxic antioxidants, was used as a carbon source to produce carbon quantum dots (CQD). Chemical analysis was conducted in accordance with AOAC (2005) recommendations. Rumen fluid from recently slaughtered calves is used to produce in vitro gas immediately following slaughter. Analysis of variance (ANOVA) was performed on the obtained data from the in vitro study in a completely randomized design using the mixed model of SAS (version 9.4; Inc., Cary NC, USA).Results and DiscussionThe variance analysis results and the average values of the chemical compositions were significantly influenced by the extracts (all p < 0.0001). In this line, the values of net gas, pH, OMD, ME, NEl, and ME were found to be the highest for Algae + 50 MgO and the lowest for Algae + 50 MgS, respectively (all p < 0.0001). These promising results imply that extracts from C. Reinhardtii may be able to mitigate the adverse consequences of rumen fermentation. To precisely ascertain the impact particular Rhodophyta on greenhouse gas emissions, additional investigation is needed.https://www.frontiersin.org/articles/10.3389/fvets.2025.1492230/fullgas productionnanoparticlesmethane emissionin vitrogreen algae |
| spellingShingle | Valiollah Palangi Adem Kaya Muhlis Macit Hayrunnisa Nadaroglu Hayrullah Bora Ünlü Ali Kaya Ashkan Fekri Ayaz Mammadov Maximilian Lackner Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production Frontiers in Veterinary Science gas production nanoparticles methane emission in vitro green algae |
| title | Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production |
| title_full | Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production |
| title_fullStr | Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production |
| title_full_unstemmed | Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production |
| title_short | Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production |
| title_sort | comparative anti methanogenic ability of green algae c reinhardtii with without nanoparticles in vitro gas and methane production |
| topic | gas production nanoparticles methane emission in vitro green algae |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1492230/full |
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