Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1
Vascular adhesion protein-1 (VAP-1) is an ectoenzyme that functions as a copper-containing amine oxidase and is involved in leukocyte adhesion at sites of inflammation. Inhibition of VAP-1 oxidative deamination has become an attractive target for anti-inflammatory therapy with demonstrated efficacy...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/3270513 |
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| author | Ryo Kubota Michael J. Reid Kuo Lee Lieu Mark Orme Christine Diamond Niklas Tulberg Susan H. Henry |
| author_facet | Ryo Kubota Michael J. Reid Kuo Lee Lieu Mark Orme Christine Diamond Niklas Tulberg Susan H. Henry |
| author_sort | Ryo Kubota |
| collection | DOAJ |
| description | Vascular adhesion protein-1 (VAP-1) is an ectoenzyme that functions as a copper-containing amine oxidase and is involved in leukocyte adhesion at sites of inflammation. Inhibition of VAP-1 oxidative deamination has become an attractive target for anti-inflammatory therapy with demonstrated efficacy in rodent models of inflammation. A previous comparison of purified recombinant VAP-1 from mouse, rat, monkey, and human gene sequences predicted that rodent VAP-1 would have higher affinity for smaller hydrophilic substrates/inhibitors because of its narrower and more hydrophilic active site channel. An optimized in vitro oxidative deamination fluorescence assay with benzylamine (BA) was used to compare inhibition of five known inhibitors in recombinant mouse, rat, and human VAP-1. Human VAP-1 was more sensitive compared to rat or mouse VAP-1 (lowest IC50 concentration) to semicarbazide but was least sensitive to hydralazine and LJP-1207. Hydralazine had a lower IC50 in rats compared to humans, although not significant. However, the IC50 of hydralazine was significantly higher in the rat compared to mouse VAP-1. The larger hydrophobic compounds from Astellas (compound 35c) and Boehringer Ingelheim (PXS-4728A) were hypothesized to have higher binding affinity for human VAP-1 compared to rodent VAP-1 since the channel in human VAP-1 is larger and more hydrophobic than that in rodent VAP-1. Although the sensitivity of these two inhibitors was the lowest in the mouse enzyme, we found no significant differences between mouse, rat, and human VAP-1. Michaelis-Menten kinetics of the small primary amines phenylethylamine and tyramine were also compared to the common marker substrate BA demonstrating that BA had the highest affinity among the substrates. Rat VAP-1 had the highest affinity for all three substrates and mouse VAP-1 had intermediate affinity for BA and phenylethylamine, but tyramine was not a substrate for mouse VAP-1 under these assay conditions. These results suggest that comparing oxidative deamination in mouse and rat VAP-1 may be important if using these species for preclinical efficacy models. |
| format | Article |
| id | doaj-art-a6863273c2a2437bb2d4e13adc05d7d6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a6863273c2a2437bb2d4e13adc05d7d62025-02-03T01:00:05ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/32705133270513Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1Ryo Kubota0Michael J. Reid1Kuo Lee Lieu2Mark Orme3Christine Diamond4Niklas Tulberg5Susan H. Henry6Acucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAAcucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAAcucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAAcucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAAcucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAAcucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAAcucela Inc., 818 Stewart St., Suite 1110, Seattle, WA 98101, USAVascular adhesion protein-1 (VAP-1) is an ectoenzyme that functions as a copper-containing amine oxidase and is involved in leukocyte adhesion at sites of inflammation. Inhibition of VAP-1 oxidative deamination has become an attractive target for anti-inflammatory therapy with demonstrated efficacy in rodent models of inflammation. A previous comparison of purified recombinant VAP-1 from mouse, rat, monkey, and human gene sequences predicted that rodent VAP-1 would have higher affinity for smaller hydrophilic substrates/inhibitors because of its narrower and more hydrophilic active site channel. An optimized in vitro oxidative deamination fluorescence assay with benzylamine (BA) was used to compare inhibition of five known inhibitors in recombinant mouse, rat, and human VAP-1. Human VAP-1 was more sensitive compared to rat or mouse VAP-1 (lowest IC50 concentration) to semicarbazide but was least sensitive to hydralazine and LJP-1207. Hydralazine had a lower IC50 in rats compared to humans, although not significant. However, the IC50 of hydralazine was significantly higher in the rat compared to mouse VAP-1. The larger hydrophobic compounds from Astellas (compound 35c) and Boehringer Ingelheim (PXS-4728A) were hypothesized to have higher binding affinity for human VAP-1 compared to rodent VAP-1 since the channel in human VAP-1 is larger and more hydrophobic than that in rodent VAP-1. Although the sensitivity of these two inhibitors was the lowest in the mouse enzyme, we found no significant differences between mouse, rat, and human VAP-1. Michaelis-Menten kinetics of the small primary amines phenylethylamine and tyramine were also compared to the common marker substrate BA demonstrating that BA had the highest affinity among the substrates. Rat VAP-1 had the highest affinity for all three substrates and mouse VAP-1 had intermediate affinity for BA and phenylethylamine, but tyramine was not a substrate for mouse VAP-1 under these assay conditions. These results suggest that comparing oxidative deamination in mouse and rat VAP-1 may be important if using these species for preclinical efficacy models.http://dx.doi.org/10.1155/2020/3270513 |
| spellingShingle | Ryo Kubota Michael J. Reid Kuo Lee Lieu Mark Orme Christine Diamond Niklas Tulberg Susan H. Henry Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1 Mediators of Inflammation |
| title | Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1 |
| title_full | Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1 |
| title_fullStr | Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1 |
| title_full_unstemmed | Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1 |
| title_short | Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1 |
| title_sort | comparison of inhibitor and substrate selectivity between rodent and human vascular adhesion protein 1 |
| url | http://dx.doi.org/10.1155/2020/3270513 |
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