Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk
Using chromosome C-banding and electrophoresis of grain storage proteins, gliadins, 17 Triticum aestivumAegilops columnaris lines with substitutions of chromosomes of homoeologous groups 1 and 6 were examined. Based on their high polymorphism, gliadins were used to identify alien genetic material. F...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-08-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1583 |
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| author | A. Yu. Novoselskaya-Dragovich A. A. Yankovskaya E. D. Badaeva |
| author_facet | A. Yu. Novoselskaya-Dragovich A. A. Yankovskaya E. D. Badaeva |
| author_sort | A. Yu. Novoselskaya-Dragovich |
| collection | DOAJ |
| description | Using chromosome C-banding and electrophoresis of grain storage proteins, gliadins, 17 Triticum aestivumAegilops columnaris lines with substitutions of chromosomes of homoeologous groups 1 and 6 were examined. Based on their high polymorphism, gliadins were used to identify alien genetic material. For all of the lines examined, electrophoretic analysis of gliadin spectra confirmed substitution of wheat chromosomes 6A, 6D or 1D for the homoeologous Aegilops chromosomes of genomes Uс or Xс. The substitution manifested in the disappearance of the products of gliadin-coding genes on chromosomes 6A, 6D or 1D with the simultaneous appearance of the products of genes localized on alien chromosomes of genomes Uс or Xс. Thus, Aegilops chromosomes were shown to be functionally active in the alien wheat genome. The absence of alien genes expression in the lines carrying a long arm deletion in chromosome 6Xc suggested that the gliadin-coding locus moved from the short chromosome arm (its characteristic position in all known wheat species) to the long one. This is probably associated with a large species-specific pericentric inversion. In spite of losing a part of its long arm and combination with a non-homologous chromosome of a different genome (4BL), chromosome 1D was fully functioning. For Aegilops, the block type of gliadin components inheritance was shown, indicating similarity in the structural organization of gliadin-coding loci in these genera. Based on determining genetic control of various polypeptides in the electrophoretic aegilops spectrum, markers to identify Ae. columnaris chromosomes 1Xс, 6Xс and 6Uс were constructed. |
| format | Article |
| id | doaj-art-a66f06e20f3045949a52b605a7eae644 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-a66f06e20f3045949a52b605a7eae6442025-02-01T09:58:06ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-08-0122550751410.18699/VJ18.388785Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris ZhukA. Yu. Novoselskaya-Dragovich0A. A. Yankovskaya1E. D. Badaeva2Vavilov Institute of General Genetics, RASVavilov Institute of General Genetics, RASVavilov Institute of General Genetics, RASUsing chromosome C-banding and electrophoresis of grain storage proteins, gliadins, 17 Triticum aestivumAegilops columnaris lines with substitutions of chromosomes of homoeologous groups 1 and 6 were examined. Based on their high polymorphism, gliadins were used to identify alien genetic material. For all of the lines examined, electrophoretic analysis of gliadin spectra confirmed substitution of wheat chromosomes 6A, 6D or 1D for the homoeologous Aegilops chromosomes of genomes Uс or Xс. The substitution manifested in the disappearance of the products of gliadin-coding genes on chromosomes 6A, 6D or 1D with the simultaneous appearance of the products of genes localized on alien chromosomes of genomes Uс or Xс. Thus, Aegilops chromosomes were shown to be functionally active in the alien wheat genome. The absence of alien genes expression in the lines carrying a long arm deletion in chromosome 6Xc suggested that the gliadin-coding locus moved from the short chromosome arm (its characteristic position in all known wheat species) to the long one. This is probably associated with a large species-specific pericentric inversion. In spite of losing a part of its long arm and combination with a non-homologous chromosome of a different genome (4BL), chromosome 1D was fully functioning. For Aegilops, the block type of gliadin components inheritance was shown, indicating similarity in the structural organization of gliadin-coding loci in these genera. Based on determining genetic control of various polypeptides in the electrophoretic aegilops spectrum, markers to identify Ae. columnaris chromosomes 1Xс, 6Xс and 6Uс were constructed.https://vavilov.elpub.ru/jour/article/view/1583electrophoresis of gliadin seed storage proteinsgliadinsc-bandingsubstitutiontranslocationaegilops columnariswheatintrogression |
| spellingShingle | A. Yu. Novoselskaya-Dragovich A. A. Yankovskaya E. D. Badaeva Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk Вавиловский журнал генетики и селекции electrophoresis of gliadin seed storage proteins gliadins c-banding substitution translocation aegilops columnaris wheat introgression |
| title | Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk |
| title_full | Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk |
| title_fullStr | Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk |
| title_full_unstemmed | Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk |
| title_short | Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk |
| title_sort | alien introgressions and chromosomal rearrangements do not affect the activity of gliadin coding genes in hybrid lines of triticum aestivum l aegilops columnaris zhuk |
| topic | electrophoresis of gliadin seed storage proteins gliadins c-banding substitution translocation aegilops columnaris wheat introgression |
| url | https://vavilov.elpub.ru/jour/article/view/1583 |
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