EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA
Medicinal plants have been playing a crucial role in drug discovery and development against infectious diseases. The antibacterial activity of methanol crude extract of traditionally used Trichilia dregeana Sond was evaluated against common bacterial pathogens. Agar well diffusion and broth dilutio...
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Scientific Veterinary Institute “Novi Sad”
2024-12-01
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| Series: | Archives of Veterinary Medicine |
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| Online Access: | https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/358 |
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| author | Tesfa Mossie Temesgen Kasa Bekesis Urge Hilena Yifred |
| author_facet | Tesfa Mossie Temesgen Kasa Bekesis Urge Hilena Yifred |
| author_sort | Tesfa Mossie |
| collection | DOAJ |
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Medicinal plants have been playing a crucial role in drug discovery and development against infectious diseases. The antibacterial activity of methanol crude extract of traditionally used Trichilia dregeana Sond was evaluated against common bacterial pathogens. Agar well diffusion and broth dilution assays were used to evaluate the antimicrobial activity and MIC of crude methanol plant extracts. The methanol crude extract of Trichilia dregeana Sond showed promising antibacterial activity against all tested bacterial isolates. A significant difference (p < 0.05) was observed in susceptibility among Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa species isolates. A higher mean susceptibility or inhibition zone diameter to crude extracts was observed in Escherichia coli (19.6 ± 5.9 mm) and Pseudomonas aeruginosa (18.4 ± 5.9 mm) compared to Staphylococcus aureus isolates. Significant differences were also observed in the susceptibility of tested bacterial isolates at different concentrations of crude extracts. A higher inhibition zone diameter was recorded in Pseudomonas aeruginosa (27.2 ± 1.5 mm), followed by Escherichia coli (25.2 ± 1.5 mm), and Staphylococcus aureus (21.1 ± 1.4 mm) at a concentration of 100 mg/mL. A comparable inhibition zone of crude extracts was observed with that of the positive control at 100 mg/mL concentration against Staphylococcus aureus and Escherichia coli isolates. The findings indicated that bacterial growth inhibition increased as the concentration of the crude extracts increased. All tested pathogenic bacteria species (gram-positive and gram-negative) were susceptible to plant leaf extract, even at lower levels of concentration. This showed the plant extracts’ broad-spectrum activity against both gram-positive and gram-negative bacteria. The methanol crude extract of plants is an effective antibacterial agent for treating bacterial infections since the extract showed significant antibacterial potency comparable with that of the standard antibiotic disk. The findings are promising and encourage further investigation of the crude extracts’ phytochemical, toxicological, and pharmacological aspects to support their potential rational use in antimicrobial therapy.
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| format | Article |
| id | doaj-art-a6180a6e6ee74103993516ba3136df98 |
| institution | Kabale University |
| issn | 1820-9955 2683-4138 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Scientific Veterinary Institute “Novi Sad” |
| record_format | Article |
| series | Archives of Veterinary Medicine |
| spelling | doaj-art-a6180a6e6ee74103993516ba3136df982025-01-19T23:28:09ZengScientific Veterinary Institute “Novi Sad”Archives of Veterinary Medicine1820-99552683-41382024-12-0117210.46784/e-avm.v17i2.358EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIATesfa Mossie0Temesgen KasaBekesis Urge1Hilena Yifred2Ethiopian institute of agriculture research, Jimma, EthiopiaEthiopian Institute of Agricultural Research, Holeta Research Center, Holeta, EthiopiaEthiopian Institute of Agriculture research Medicinal plants have been playing a crucial role in drug discovery and development against infectious diseases. The antibacterial activity of methanol crude extract of traditionally used Trichilia dregeana Sond was evaluated against common bacterial pathogens. Agar well diffusion and broth dilution assays were used to evaluate the antimicrobial activity and MIC of crude methanol plant extracts. The methanol crude extract of Trichilia dregeana Sond showed promising antibacterial activity against all tested bacterial isolates. A significant difference (p < 0.05) was observed in susceptibility among Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa species isolates. A higher mean susceptibility or inhibition zone diameter to crude extracts was observed in Escherichia coli (19.6 ± 5.9 mm) and Pseudomonas aeruginosa (18.4 ± 5.9 mm) compared to Staphylococcus aureus isolates. Significant differences were also observed in the susceptibility of tested bacterial isolates at different concentrations of crude extracts. A higher inhibition zone diameter was recorded in Pseudomonas aeruginosa (27.2 ± 1.5 mm), followed by Escherichia coli (25.2 ± 1.5 mm), and Staphylococcus aureus (21.1 ± 1.4 mm) at a concentration of 100 mg/mL. A comparable inhibition zone of crude extracts was observed with that of the positive control at 100 mg/mL concentration against Staphylococcus aureus and Escherichia coli isolates. The findings indicated that bacterial growth inhibition increased as the concentration of the crude extracts increased. All tested pathogenic bacteria species (gram-positive and gram-negative) were susceptible to plant leaf extract, even at lower levels of concentration. This showed the plant extracts’ broad-spectrum activity against both gram-positive and gram-negative bacteria. The methanol crude extract of plants is an effective antibacterial agent for treating bacterial infections since the extract showed significant antibacterial potency comparable with that of the standard antibiotic disk. The findings are promising and encourage further investigation of the crude extracts’ phytochemical, toxicological, and pharmacological aspects to support their potential rational use in antimicrobial therapy. https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/358Antibacterial activitycrude extractsisolatesMICTrichilia dregeana Sond |
| spellingShingle | Tesfa Mossie Temesgen Kasa Bekesis Urge Hilena Yifred EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA Archives of Veterinary Medicine Antibacterial activity crude extracts isolates MIC Trichilia dregeana Sond |
| title | EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA |
| title_full | EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA |
| title_fullStr | EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA |
| title_full_unstemmed | EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA |
| title_short | EVALUATION OF ANTIBACTERIAL ACTIVITY OF TRICHILIA DREGEANA SOND (MELIACEAE) CRUDE EXTRACTS AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA |
| title_sort | evaluation of antibacterial activity of trichilia dregeana sond meliaceae crude extracts against gram positive and gram negative bacteria |
| topic | Antibacterial activity crude extracts isolates MIC Trichilia dregeana Sond |
| url | https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/358 |
| work_keys_str_mv | AT tesfamossie evaluationofantibacterialactivityoftrichiliadregeanasondmeliaceaecrudeextractsagainstgrampositiveandgramnegativebacteria AT temesgenkasa evaluationofantibacterialactivityoftrichiliadregeanasondmeliaceaecrudeextractsagainstgrampositiveandgramnegativebacteria AT bekesisurge evaluationofantibacterialactivityoftrichiliadregeanasondmeliaceaecrudeextractsagainstgrampositiveandgramnegativebacteria AT hilenayifred evaluationofantibacterialactivityoftrichiliadregeanasondmeliaceaecrudeextractsagainstgrampositiveandgramnegativebacteria |