Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins

Orange (Citrus sinensis) juice contains a high amount of antioxidant compounds, such as polyphenols and vitamins. The aim of this work was to develop an adsorption procedure for the quantitative recovery of polyphenols from fresh orange juice. Different macroporous resins have been selected to evalu...

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Main Authors: Luca Campone, Rita Celano, Serena Rizzo, Anna Lisa Piccinelli, Luca Rastrelli, Mariateresa Russo
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Food Quality
Online Access:http://dx.doi.org/10.1155/2020/1251957
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author Luca Campone
Rita Celano
Serena Rizzo
Anna Lisa Piccinelli
Luca Rastrelli
Mariateresa Russo
author_facet Luca Campone
Rita Celano
Serena Rizzo
Anna Lisa Piccinelli
Luca Rastrelli
Mariateresa Russo
author_sort Luca Campone
collection DOAJ
description Orange (Citrus sinensis) juice contains a high amount of antioxidant compounds, such as polyphenols and vitamins. The aim of this work was to develop an adsorption procedure for the quantitative recovery of polyphenols from fresh orange juice. Different macroporous resins have been selected to evaluate their affinity for phenolic compound in order to purify the antioxidant compounds from the orange juice. The main compounds of orange juice were firstly characterized using an UPLC-UV-HRMS to define the metabolite profile, and subsequently three different types of adsorbent (XAD-2, XAD-4, and XAD-16N) were tested to concentrate these bioactive compounds. The time of contact was selected based on kinetic studies, and subsequently the adsorption and elution conditions were optimized in order to maximize the recovery of phenolic compounds to obtain an extract rich of bioactive compounds. Lastly, antioxidant capacity of the orange juice extract of selected macroporous resin, obtained under optimized conditions, was determined by in vitro antioxidant assays.
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institution Kabale University
issn 0146-9428
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publishDate 2020-01-01
publisher Wiley
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series Journal of Food Quality
spelling doaj-art-a5c20f4d8bed43af8eb1fca9c8be81e32025-02-03T06:46:24ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/12519571251957Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous ResinsLuca Campone0Rita Celano1Serena Rizzo2Anna Lisa Piccinelli3Luca Rastrelli4Mariateresa Russo5Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza Della Scienza 2, I-20126 Milan, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II, Fisciano, SA, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II, Fisciano, SA, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II, Fisciano, SA, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II, Fisciano, SA, ItalyDepartment of Agriculture, Food Chemistry Safety and Sensoromic Laboratory (FoCuSS Lab), University of Mediterranea, Via Melissari, Reggio Calabria, RC, ItalyOrange (Citrus sinensis) juice contains a high amount of antioxidant compounds, such as polyphenols and vitamins. The aim of this work was to develop an adsorption procedure for the quantitative recovery of polyphenols from fresh orange juice. Different macroporous resins have been selected to evaluate their affinity for phenolic compound in order to purify the antioxidant compounds from the orange juice. The main compounds of orange juice were firstly characterized using an UPLC-UV-HRMS to define the metabolite profile, and subsequently three different types of adsorbent (XAD-2, XAD-4, and XAD-16N) were tested to concentrate these bioactive compounds. The time of contact was selected based on kinetic studies, and subsequently the adsorption and elution conditions were optimized in order to maximize the recovery of phenolic compounds to obtain an extract rich of bioactive compounds. Lastly, antioxidant capacity of the orange juice extract of selected macroporous resin, obtained under optimized conditions, was determined by in vitro antioxidant assays.http://dx.doi.org/10.1155/2020/1251957
spellingShingle Luca Campone
Rita Celano
Serena Rizzo
Anna Lisa Piccinelli
Luca Rastrelli
Mariateresa Russo
Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins
Journal of Food Quality
title Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins
title_full Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins
title_fullStr Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins
title_full_unstemmed Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins
title_short Development of an Enriched Polyphenol (Natural Antioxidant) Extract from Orange Juice (Citrus sinensis) by Adsorption on Macroporous Resins
title_sort development of an enriched polyphenol natural antioxidant extract from orange juice citrus sinensis by adsorption on macroporous resins
url http://dx.doi.org/10.1155/2020/1251957
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