Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis
Abstract Mycobacterium tuberculosis (M. tb) has a remarkable ability to persist inside host cells. Several studies showed that M. tb infects and survives inside bone marrow mesenchymal stem cells (BM-MSCs) escaping the host immune system. Here, we have identified various cellular pathways that are m...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-75722-5 |
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| author | Simran Kaur Nupur Angrish Madavan Vasudevan Garima Khare |
| author_facet | Simran Kaur Nupur Angrish Madavan Vasudevan Garima Khare |
| author_sort | Simran Kaur |
| collection | DOAJ |
| description | Abstract Mycobacterium tuberculosis (M. tb) has a remarkable ability to persist inside host cells. Several studies showed that M. tb infects and survives inside bone marrow mesenchymal stem cells (BM-MSCs) escaping the host immune system. Here, we have identified various cellular pathways that are modulated in human BM-MSCs upon infection with virulent M. tb and the proteomic profile of these cells varies from that of avirulent M. tb infected cells. We found that virulent M. tb infection reshapes host pathways such as stem cell differentiation, alternative splicing, cytokine production, mitochondrial function etc., which might be modulated by M. tb to persist inside this unconventional niche of human BM-MSCs. Additionally, we observed that virulent M. tb infection suppresses various cellular processes. This study uncovers the differences in the host proteomic profiles resulting from the virulent versus avirulent M. tb infection that can pave the way to identify host-directed therapeutic targets for the treatment of tuberculosis. |
| format | Article |
| id | doaj-art-a595b39385334a50903473e43db52d6c |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a595b39385334a50903473e43db52d6c2025-08-20T02:39:35ZengNature PortfolioScientific Reports2045-23222024-12-0114111510.1038/s41598-024-75722-5Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosisSimran Kaur0Nupur Angrish1Madavan Vasudevan2Garima Khare3Department of Biochemistry, University of Delhi South CampusDepartment of Biochemistry, University of Delhi South CampusGenomics and Data Science Unit, Theomics International Pvt. LtdDepartment of Biochemistry, University of Delhi South CampusAbstract Mycobacterium tuberculosis (M. tb) has a remarkable ability to persist inside host cells. Several studies showed that M. tb infects and survives inside bone marrow mesenchymal stem cells (BM-MSCs) escaping the host immune system. Here, we have identified various cellular pathways that are modulated in human BM-MSCs upon infection with virulent M. tb and the proteomic profile of these cells varies from that of avirulent M. tb infected cells. We found that virulent M. tb infection reshapes host pathways such as stem cell differentiation, alternative splicing, cytokine production, mitochondrial function etc., which might be modulated by M. tb to persist inside this unconventional niche of human BM-MSCs. Additionally, we observed that virulent M. tb infection suppresses various cellular processes. This study uncovers the differences in the host proteomic profiles resulting from the virulent versus avirulent M. tb infection that can pave the way to identify host-directed therapeutic targets for the treatment of tuberculosis.https://doi.org/10.1038/s41598-024-75722-5Host-directed therapeuticsMesenchymal stem cellsMycobacterium tuberculosisProteomicsPathway analysis |
| spellingShingle | Simran Kaur Nupur Angrish Madavan Vasudevan Garima Khare Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis Scientific Reports Host-directed therapeutics Mesenchymal stem cells Mycobacterium tuberculosis Proteomics Pathway analysis |
| title | Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis |
| title_full | Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis |
| title_fullStr | Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis |
| title_full_unstemmed | Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis |
| title_short | Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis |
| title_sort | global proteomics reveals pathways of mesenchymal stem cells altered by mycobacterium tuberculosis |
| topic | Host-directed therapeutics Mesenchymal stem cells Mycobacterium tuberculosis Proteomics Pathway analysis |
| url | https://doi.org/10.1038/s41598-024-75722-5 |
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