How Neutrophil Extracellular Traps Become Visible
Neutrophil extracellular traps (NETs) have been identified as a fundamental innate immune defense mechanism against different pathogens. NETs are characterized as released nuclear DNA associated with histones and granule proteins, which form an extracellular web-like structure that is able to entrap...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4604713 |
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| author | Nicole de Buhr Maren von Köckritz-Blickwede |
| author_facet | Nicole de Buhr Maren von Köckritz-Blickwede |
| author_sort | Nicole de Buhr |
| collection | DOAJ |
| description | Neutrophil extracellular traps (NETs) have been identified as a fundamental innate immune defense mechanism against different pathogens. NETs are characterized as released nuclear DNA associated with histones and granule proteins, which form an extracellular web-like structure that is able to entrap and occasionally kill certain microbes. Furthermore, NETs have been shown to contribute to several noninfectious disease conditions when released by activated neutrophils during inflammation. The identification of NETs has mainly been succeeded by various microscopy techniques, for example, immunofluorescence microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Since the last years the development and improvement of new immunofluorescence-based techniques enabled optimized visualization and quantification of NETs. On the one hand in vitro live-cell imaging led to profound new ideas about the mechanisms involved in the formation and functionality of NETs. On the other hand different intravital, in vivo, and in situ microscopy techniques led to deeper insights into the role of NET formation during health and disease. This paper presents an overview of the main used microscopy techniques to visualize NETs and describes their advantages as well as disadvantages. |
| format | Article |
| id | doaj-art-a59395738ab84b2b807b8b3abd6a7810 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a59395738ab84b2b807b8b3abd6a78102025-02-03T01:09:33ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/46047134604713How Neutrophil Extracellular Traps Become VisibleNicole de Buhr0Maren von Köckritz-Blickwede1Department of Physiological Chemistry and Department of Infectious Diseases, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, GermanyDepartment of Physiological Chemistry and Department of Infectious Diseases, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, GermanyNeutrophil extracellular traps (NETs) have been identified as a fundamental innate immune defense mechanism against different pathogens. NETs are characterized as released nuclear DNA associated with histones and granule proteins, which form an extracellular web-like structure that is able to entrap and occasionally kill certain microbes. Furthermore, NETs have been shown to contribute to several noninfectious disease conditions when released by activated neutrophils during inflammation. The identification of NETs has mainly been succeeded by various microscopy techniques, for example, immunofluorescence microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Since the last years the development and improvement of new immunofluorescence-based techniques enabled optimized visualization and quantification of NETs. On the one hand in vitro live-cell imaging led to profound new ideas about the mechanisms involved in the formation and functionality of NETs. On the other hand different intravital, in vivo, and in situ microscopy techniques led to deeper insights into the role of NET formation during health and disease. This paper presents an overview of the main used microscopy techniques to visualize NETs and describes their advantages as well as disadvantages.http://dx.doi.org/10.1155/2016/4604713 |
| spellingShingle | Nicole de Buhr Maren von Köckritz-Blickwede How Neutrophil Extracellular Traps Become Visible Journal of Immunology Research |
| title | How Neutrophil Extracellular Traps Become Visible |
| title_full | How Neutrophil Extracellular Traps Become Visible |
| title_fullStr | How Neutrophil Extracellular Traps Become Visible |
| title_full_unstemmed | How Neutrophil Extracellular Traps Become Visible |
| title_short | How Neutrophil Extracellular Traps Become Visible |
| title_sort | how neutrophil extracellular traps become visible |
| url | http://dx.doi.org/10.1155/2016/4604713 |
| work_keys_str_mv | AT nicoledebuhr howneutrophilextracellulartrapsbecomevisible AT marenvonkockritzblickwede howneutrophilextracellulartrapsbecomevisible |