Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis
ObjectivesNew biomarkers are needed to detect liver cirrhosis at an earlier stage and to individualize treatment strategies. This study specifically investigates the diagnostic potential of Ring finger protein 41 promoter methylation as an epigenetic biomarker for detecting early-stage hepatitis B v...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1631990/full |
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| author | Hanxu Zhu Feng Zhang Zhezhe Tian Miaomiao Xu Yuchen Fan Yuchen Fan Shuai Gao Kai Wang Kai Wang |
| author_facet | Hanxu Zhu Feng Zhang Zhezhe Tian Miaomiao Xu Yuchen Fan Yuchen Fan Shuai Gao Kai Wang Kai Wang |
| author_sort | Hanxu Zhu |
| collection | DOAJ |
| description | ObjectivesNew biomarkers are needed to detect liver cirrhosis at an earlier stage and to individualize treatment strategies. This study specifically investigates the diagnostic potential of Ring finger protein 41 promoter methylation as an epigenetic biomarker for detecting early-stage hepatitis B virus-related liver cirrhosis.MethodsThe methylation level of the Ring finger protein 41 promoter in peripheral blood mononuclear cells of 190 participants were quantified with Methylight, and the changes of serum inflammatory cytokines related to liver fibrosis were analyzed simultaneously.ResultsPatients with early-stage liver cirrhosis exhibited significantly higher methylation levels of Ring finger protein 41 promoter than chronic hepatitis B patients and health controls, accompanied by reduced mRNA expression. Remarkably, the receiver operating characteristic analysis demonstrated that Ring finger protein 41 promoter methylation achieved a superior diagnostic performance (area under the curve) for distinguishing hepatitis B virus-related compensated liver cirrhosis, outperforming conventional non-invasive indicators including liver stiffness measurement, aspartate aminotransferase-to-platelet ratio index, and fibrosis-4 score.ConclusionRing finger protein 41 may play a critical role in the pathogenesis of liver cirrhosis, with its methylation status in peripheral blood mononuclear cells demonstrating strong potential as a non-invasive biomarker for early liver cirrhosis detection. |
| format | Article |
| id | doaj-art-a5928e90f8fe4c3785dc4eba00d36737 |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-a5928e90f8fe4c3785dc4eba00d367372025-08-20T02:55:06ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-08-011210.3389/fmed.2025.16319901631990Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosisHanxu Zhu0Feng Zhang1Zhezhe Tian2Miaomiao Xu3Yuchen Fan4Yuchen Fan5Shuai Gao6Kai Wang7Kai Wang8Department of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaHepatology Institute of Shandong University, Jinan, Shandong, ChinaDepartment of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Hepatology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaHepatology Institute of Shandong University, Jinan, Shandong, ChinaObjectivesNew biomarkers are needed to detect liver cirrhosis at an earlier stage and to individualize treatment strategies. This study specifically investigates the diagnostic potential of Ring finger protein 41 promoter methylation as an epigenetic biomarker for detecting early-stage hepatitis B virus-related liver cirrhosis.MethodsThe methylation level of the Ring finger protein 41 promoter in peripheral blood mononuclear cells of 190 participants were quantified with Methylight, and the changes of serum inflammatory cytokines related to liver fibrosis were analyzed simultaneously.ResultsPatients with early-stage liver cirrhosis exhibited significantly higher methylation levels of Ring finger protein 41 promoter than chronic hepatitis B patients and health controls, accompanied by reduced mRNA expression. Remarkably, the receiver operating characteristic analysis demonstrated that Ring finger protein 41 promoter methylation achieved a superior diagnostic performance (area under the curve) for distinguishing hepatitis B virus-related compensated liver cirrhosis, outperforming conventional non-invasive indicators including liver stiffness measurement, aspartate aminotransferase-to-platelet ratio index, and fibrosis-4 score.ConclusionRing finger protein 41 may play a critical role in the pathogenesis of liver cirrhosis, with its methylation status in peripheral blood mononuclear cells demonstrating strong potential as a non-invasive biomarker for early liver cirrhosis detection.https://www.frontiersin.org/articles/10.3389/fmed.2025.1631990/fullRing finger protein 41hepatitis Bliver cirrhosisDNA methylationearly diagnosis |
| spellingShingle | Hanxu Zhu Feng Zhang Zhezhe Tian Miaomiao Xu Yuchen Fan Yuchen Fan Shuai Gao Kai Wang Kai Wang Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis Frontiers in Medicine Ring finger protein 41 hepatitis B liver cirrhosis DNA methylation early diagnosis |
| title | Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis |
| title_full | Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis |
| title_fullStr | Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis |
| title_full_unstemmed | Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis |
| title_short | Hypermethylation of Ring finger protein 41 promoter is associated with early hepatitis B virus-related cirrhosis |
| title_sort | hypermethylation of ring finger protein 41 promoter is associated with early hepatitis b virus related cirrhosis |
| topic | Ring finger protein 41 hepatitis B liver cirrhosis DNA methylation early diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1631990/full |
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