Modulating transformation of DNA origami nanoarray via sequence design
Abstract The four-way DNA junction is the most prevalent structural motif in DNA nanotechnology. Recently, a reconfigurable DNA nanoarray (domino array) was created with this basic motif to realize intricate, stepwise transformation by the information relay between neighboring four-way junction unit...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-61421-w |
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| author | Dongfang Wang Fiona Cole Martina Pfeiffer Mengting Cao Tim Schröder Philip Tinnefeld Yonggang Ke |
| author_facet | Dongfang Wang Fiona Cole Martina Pfeiffer Mengting Cao Tim Schröder Philip Tinnefeld Yonggang Ke |
| author_sort | Dongfang Wang |
| collection | DOAJ |
| description | Abstract The four-way DNA junction is the most prevalent structural motif in DNA nanotechnology. Recently, a reconfigurable DNA nanoarray (domino array) was created with this basic motif to realize intricate, stepwise transformation by the information relay between neighboring four-way junction units. Here, we generate a DNA domino array with same sequences at every junction, and use it as a platform to study how the design of DNA bases at junctions influences the kinetics and thermodynamics of transformation of four-way junctions in reconfigurable DNA nanoarrays. By regulating the energy difference and thus the conversion between the two configurations of four-way junctions, we show the transformation of DNA nanoarray can be modulated in a designable manner. The coordinated transformation of four-way junctions in the DNA domino array enables a detailed investigation on array transformation by using Atomic Force Microscopy (AFM) imaging and single-molecule Förster resonance energy transfer (FRET) microscopy. |
| format | Article |
| id | doaj-art-a563fae1da1b4e1a9b75e48d10b09012 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-a563fae1da1b4e1a9b75e48d10b090122025-08-20T04:01:41ZengNature PortfolioNature Communications2041-17232025-07-011611910.1038/s41467-025-61421-wModulating transformation of DNA origami nanoarray via sequence designDongfang Wang0Fiona Cole1Martina Pfeiffer2Mengting Cao3Tim Schröder4Philip Tinnefeld5Yonggang Ke6School of Biomedical Engineering, University of Science and Technology of ChinaDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität MünchenDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität MünchenSchool of Biomedical Engineering, University of Science and Technology of ChinaDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität MünchenDepartment of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität MünchenWallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of TechnologyAbstract The four-way DNA junction is the most prevalent structural motif in DNA nanotechnology. Recently, a reconfigurable DNA nanoarray (domino array) was created with this basic motif to realize intricate, stepwise transformation by the information relay between neighboring four-way junction units. Here, we generate a DNA domino array with same sequences at every junction, and use it as a platform to study how the design of DNA bases at junctions influences the kinetics and thermodynamics of transformation of four-way junctions in reconfigurable DNA nanoarrays. By regulating the energy difference and thus the conversion between the two configurations of four-way junctions, we show the transformation of DNA nanoarray can be modulated in a designable manner. The coordinated transformation of four-way junctions in the DNA domino array enables a detailed investigation on array transformation by using Atomic Force Microscopy (AFM) imaging and single-molecule Förster resonance energy transfer (FRET) microscopy.https://doi.org/10.1038/s41467-025-61421-w |
| spellingShingle | Dongfang Wang Fiona Cole Martina Pfeiffer Mengting Cao Tim Schröder Philip Tinnefeld Yonggang Ke Modulating transformation of DNA origami nanoarray via sequence design Nature Communications |
| title | Modulating transformation of DNA origami nanoarray via sequence design |
| title_full | Modulating transformation of DNA origami nanoarray via sequence design |
| title_fullStr | Modulating transformation of DNA origami nanoarray via sequence design |
| title_full_unstemmed | Modulating transformation of DNA origami nanoarray via sequence design |
| title_short | Modulating transformation of DNA origami nanoarray via sequence design |
| title_sort | modulating transformation of dna origami nanoarray via sequence design |
| url | https://doi.org/10.1038/s41467-025-61421-w |
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