Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak.
The heart develops from 2 sources of mesoderm progenitors, the first and second heart field (FHF and SHF). Using a single-cell transcriptomic assay combined with genetic lineage tracing and live imaging, we find the FHF and SHF are subdivided into distinct pools of progenitors in gastrulating mouse...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-05-01
|
| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001200&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850129729514897408 |
|---|---|
| author | Kenzo Ivanovitch Pablo Soro-Barrio Probir Chakravarty Rebecca A Jones Donald M Bell S Neda Mousavy Gharavy Despina Stamataki Julien Delile James C Smith James Briscoe |
| author_facet | Kenzo Ivanovitch Pablo Soro-Barrio Probir Chakravarty Rebecca A Jones Donald M Bell S Neda Mousavy Gharavy Despina Stamataki Julien Delile James C Smith James Briscoe |
| author_sort | Kenzo Ivanovitch |
| collection | DOAJ |
| description | The heart develops from 2 sources of mesoderm progenitors, the first and second heart field (FHF and SHF). Using a single-cell transcriptomic assay combined with genetic lineage tracing and live imaging, we find the FHF and SHF are subdivided into distinct pools of progenitors in gastrulating mouse embryos at earlier stages than previously thought. Each subpopulation has a distinct origin in the primitive streak. The first progenitors to leave the primitive streak contribute to the left ventricle, shortly after right ventricle progenitor emigrate, followed by the outflow tract and atrial progenitors. Moreover, a subset of atrial progenitors are gradually incorporated in posterior locations of the FHF. Although cells allocated to the outflow tract and atrium leave the primitive streak at a similar stage, they arise from different regions. Outflow tract cells originate from distal locations in the primitive streak while atrial progenitors are positioned more proximally. Moreover, single-cell RNA sequencing demonstrates that the primitive streak cells contributing to the ventricles have a distinct molecular signature from those forming the outflow tract and atrium. We conclude that cardiac progenitors are prepatterned within the primitive streak and this prefigures their allocation to distinct anatomical structures of the heart. Together, our data provide a new molecular and spatial map of mammalian cardiac progenitors that will support future studies of heart development, function, and disease. |
| format | Article |
| id | doaj-art-a5429e0ee4a848d2b0cec818d86e57c3 |
| institution | OA Journals |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-a5429e0ee4a848d2b0cec818d86e57c32025-08-20T02:32:53ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852021-05-01195e300120010.1371/journal.pbio.3001200Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak.Kenzo IvanovitchPablo Soro-BarrioProbir ChakravartyRebecca A JonesDonald M BellS Neda Mousavy GharavyDespina StamatakiJulien DelileJames C SmithJames BriscoeThe heart develops from 2 sources of mesoderm progenitors, the first and second heart field (FHF and SHF). Using a single-cell transcriptomic assay combined with genetic lineage tracing and live imaging, we find the FHF and SHF are subdivided into distinct pools of progenitors in gastrulating mouse embryos at earlier stages than previously thought. Each subpopulation has a distinct origin in the primitive streak. The first progenitors to leave the primitive streak contribute to the left ventricle, shortly after right ventricle progenitor emigrate, followed by the outflow tract and atrial progenitors. Moreover, a subset of atrial progenitors are gradually incorporated in posterior locations of the FHF. Although cells allocated to the outflow tract and atrium leave the primitive streak at a similar stage, they arise from different regions. Outflow tract cells originate from distal locations in the primitive streak while atrial progenitors are positioned more proximally. Moreover, single-cell RNA sequencing demonstrates that the primitive streak cells contributing to the ventricles have a distinct molecular signature from those forming the outflow tract and atrium. We conclude that cardiac progenitors are prepatterned within the primitive streak and this prefigures their allocation to distinct anatomical structures of the heart. Together, our data provide a new molecular and spatial map of mammalian cardiac progenitors that will support future studies of heart development, function, and disease.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001200&type=printable |
| spellingShingle | Kenzo Ivanovitch Pablo Soro-Barrio Probir Chakravarty Rebecca A Jones Donald M Bell S Neda Mousavy Gharavy Despina Stamataki Julien Delile James C Smith James Briscoe Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. PLoS Biology |
| title | Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. |
| title_full | Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. |
| title_fullStr | Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. |
| title_full_unstemmed | Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. |
| title_short | Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. |
| title_sort | ventricular atrial and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001200&type=printable |
| work_keys_str_mv | AT kenzoivanovitch ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT pablosorobarrio ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT probirchakravarty ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT rebeccaajones ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT donaldmbell ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT snedamousavygharavy ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT despinastamataki ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT juliendelile ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT jamescsmith ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak AT jamesbriscoe ventricularatrialandoutflowtractheartprogenitorsarisefromspatiallyandmolecularlydistinctregionsoftheprimitivestreak |