Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box
Stevia rebaudiana Bertoni is a member of Compositae family. Stevia plant has zero calorie content and its leaves are estimated to be 300 times sweeter than sugar. This plant is believed to be the most ideal substitute for sugar and important to assist in medicinal value especially for diabetic patie...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/940201 |
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| author | Mohamad Osman Nur Syamimi Samsudin Golam Faruq Arash Nezhadahmadi |
| author_facet | Mohamad Osman Nur Syamimi Samsudin Golam Faruq Arash Nezhadahmadi |
| author_sort | Mohamad Osman |
| collection | DOAJ |
| description | Stevia rebaudiana Bertoni is a member of Compositae family. Stevia plant has zero calorie content and its leaves are estimated to be 300 times sweeter than sugar. This plant is believed to be the most ideal substitute for sugar and important to assist in medicinal value especially for diabetic patients. In this study, microcutting techniques using a mist-chamber propagation box were used as it was beneficial for propagation of Stevia and gave genetic uniformity to the plant. The effects of different treatments on root stimulation of Stevia in microcuttings technique were evaluated. Treatments studied were different sizes of shoot cuttings, plant growth regulators, lights, and shades. Data logger was used to record the mean value of humidity (>90% RH), light intensity (673–2045 lx), and temperature (28.6–30.1°C) inside the mist-chamber propagation box. From analysis of variance, there were significant differences between varieties and treatments in parameters studied (P<0.05). For the size of shoot cuttings treatment, 6 nodes cuttings were observed to increase root number. As compared to control, shoot cuttings treated with indole butyric acid (IBA) had better performance regarding root length. Yellow light and 50% shade treatments showed higher root and leaf number and these conditions can be considered as crucial for potential propagation of Stevia. |
| format | Article |
| id | doaj-art-a54016c0fd2b43e7a2e2fcdfc9f07c11 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a54016c0fd2b43e7a2e2fcdfc9f07c112025-08-20T02:23:04ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/940201940201Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation BoxMohamad Osman0Nur Syamimi Samsudin1Golam Faruq2Arash Nezhadahmadi3Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, MalaysiaFaculty of Science, International Islamic University, 25200 Kuantan, Pahang, MalaysiaInstitute of Biological Sciences, Faculty of Science, University Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Biological Sciences, Faculty of Science, University Malaya, 50603 Kuala Lumpur, MalaysiaStevia rebaudiana Bertoni is a member of Compositae family. Stevia plant has zero calorie content and its leaves are estimated to be 300 times sweeter than sugar. This plant is believed to be the most ideal substitute for sugar and important to assist in medicinal value especially for diabetic patients. In this study, microcutting techniques using a mist-chamber propagation box were used as it was beneficial for propagation of Stevia and gave genetic uniformity to the plant. The effects of different treatments on root stimulation of Stevia in microcuttings technique were evaluated. Treatments studied were different sizes of shoot cuttings, plant growth regulators, lights, and shades. Data logger was used to record the mean value of humidity (>90% RH), light intensity (673–2045 lx), and temperature (28.6–30.1°C) inside the mist-chamber propagation box. From analysis of variance, there were significant differences between varieties and treatments in parameters studied (P<0.05). For the size of shoot cuttings treatment, 6 nodes cuttings were observed to increase root number. As compared to control, shoot cuttings treated with indole butyric acid (IBA) had better performance regarding root length. Yellow light and 50% shade treatments showed higher root and leaf number and these conditions can be considered as crucial for potential propagation of Stevia.http://dx.doi.org/10.1155/2013/940201 |
| spellingShingle | Mohamad Osman Nur Syamimi Samsudin Golam Faruq Arash Nezhadahmadi Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box The Scientific World Journal |
| title | Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box |
| title_full | Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box |
| title_fullStr | Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box |
| title_full_unstemmed | Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box |
| title_short | Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box |
| title_sort | factors affecting microcuttings of stevia using a mist chamber propagation box |
| url | http://dx.doi.org/10.1155/2013/940201 |
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