QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture
Abstract Spike-related traits and plant height (PH) are greatly associated with wheat yield. Identification of stable quantitative trait loci (QTL) for these traits is crucial for understanding the genetic basis for yield and their further application in breeding. In this study, QTL analysis for spi...
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BMC
2024-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-05885-w |
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| author | Baowei Chen Yuanjiang He Yanyan Tang Zhengxi Lin Jinhui Wang Juanyu Zhang Junjun Liang Haili Zhang Guangbing Deng Yong Ren Tao Li Hai Long |
| author_facet | Baowei Chen Yuanjiang He Yanyan Tang Zhengxi Lin Jinhui Wang Juanyu Zhang Junjun Liang Haili Zhang Guangbing Deng Yong Ren Tao Li Hai Long |
| author_sort | Baowei Chen |
| collection | DOAJ |
| description | Abstract Spike-related traits and plant height (PH) are greatly associated with wheat yield. Identification of stable quantitative trait loci (QTL) for these traits is crucial for understanding the genetic basis for yield and their further application in breeding. In this study, QTL analysis for spikelet number per spike (SNS), spike length (SL), spike compactness (SC) and PH was performed using a recombinant inbred line (RIL) population derived from a cross between wheat cultivars Mianmai902 (MM902) and Taichang29 (TC29). Thirteen stably expressed QTLs were identified, and the most favorable alleles were contributed by MM902. The mjaor QTL, QSNS-MT.cib-2D, QSL-MT.cib-2D, QSC-MT.cib-2D, QSC-MT.cib-6 A, QPH-MT.cib-4B and QPH-MT.cib-4D, were repeatedly detected in multiple environments and explained 5.77-47.11% of the phenotypic variations. By employing the Kompetitive Allele Specific PCR markers, the most major QTLs were successfully validated in multiple populations derived from different genetic backgrounds. Moreover, the individual and pyramiding effects of major QTLs on SNS, SL, SC, PH, grain number per spike (GNS) and thousand-grain weight (TGW) were investigated and their potential utilization value in breeding was showcased. Ppd-D1 was predicted as the candidate for QSNS/SL/SC/PH-MT.cib-2D, and MM902 carried the Ppd-D1d allele. Ppd-D1d is rare in Chinese winter wheat cultivars and may be an elite alternative allele of the Ppd-D1a allele. In summary, these QTLs revealed the genetic basis of the spike-related traits and PH of MM902, which partially contributed to the high yield of MM902 and have application potential in wheat breeding by optimizing spike morphology and PH to improve yield. |
| format | Article |
| id | doaj-art-a4e5e8f46d294f0785f26d3e9d6dce1b |
| institution | DOAJ |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-a4e5e8f46d294f0785f26d3e9d6dce1b2025-08-20T02:40:21ZengBMCBMC Plant Biology1471-22292024-12-0124111510.1186/s12870-024-05885-wQTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architectureBaowei Chen0Yuanjiang He1Yanyan Tang2Zhengxi Lin3Jinhui Wang4Juanyu Zhang5Junjun Liang6Haili Zhang7Guangbing Deng8Yong Ren9Tao Li10Hai Long11Chengdu Institute of Biology, Chinese Academy of SciencesMianyang Academy of Agricultural SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesMianyang Academy of Agricultural SciencesChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesAbstract Spike-related traits and plant height (PH) are greatly associated with wheat yield. Identification of stable quantitative trait loci (QTL) for these traits is crucial for understanding the genetic basis for yield and their further application in breeding. In this study, QTL analysis for spikelet number per spike (SNS), spike length (SL), spike compactness (SC) and PH was performed using a recombinant inbred line (RIL) population derived from a cross between wheat cultivars Mianmai902 (MM902) and Taichang29 (TC29). Thirteen stably expressed QTLs were identified, and the most favorable alleles were contributed by MM902. The mjaor QTL, QSNS-MT.cib-2D, QSL-MT.cib-2D, QSC-MT.cib-2D, QSC-MT.cib-6 A, QPH-MT.cib-4B and QPH-MT.cib-4D, were repeatedly detected in multiple environments and explained 5.77-47.11% of the phenotypic variations. By employing the Kompetitive Allele Specific PCR markers, the most major QTLs were successfully validated in multiple populations derived from different genetic backgrounds. Moreover, the individual and pyramiding effects of major QTLs on SNS, SL, SC, PH, grain number per spike (GNS) and thousand-grain weight (TGW) were investigated and their potential utilization value in breeding was showcased. Ppd-D1 was predicted as the candidate for QSNS/SL/SC/PH-MT.cib-2D, and MM902 carried the Ppd-D1d allele. Ppd-D1d is rare in Chinese winter wheat cultivars and may be an elite alternative allele of the Ppd-D1a allele. In summary, these QTLs revealed the genetic basis of the spike-related traits and PH of MM902, which partially contributed to the high yield of MM902 and have application potential in wheat breeding by optimizing spike morphology and PH to improve yield.https://doi.org/10.1186/s12870-024-05885-wYield potentialQTL detectionSpikePlant heightMarker-assisted selection |
| spellingShingle | Baowei Chen Yuanjiang He Yanyan Tang Zhengxi Lin Jinhui Wang Juanyu Zhang Junjun Liang Haili Zhang Guangbing Deng Yong Ren Tao Li Hai Long QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture BMC Plant Biology Yield potential QTL detection Spike Plant height Marker-assisted selection |
| title | QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture |
| title_full | QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture |
| title_fullStr | QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture |
| title_full_unstemmed | QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture |
| title_short | QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture |
| title_sort | qtl analysis in the mianmai 902 taichang 29 ril population reveals the genetic basis for the high yield of wheat cultivars mianmai 902 in terms of spike and plant architecture |
| topic | Yield potential QTL detection Spike Plant height Marker-assisted selection |
| url | https://doi.org/10.1186/s12870-024-05885-w |
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