Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare
A variety of dietary nonalcoholic steatohepatitis (NASH) mouse models are available, and choosing the appropriate mouse model is one of the most important steps in the design of NASH studies. In addition to the histopathological and metabolic findings of NASH, a sufficient mouse model should guarant...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2020/7347068 |
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| author | Andreas Kroh Vanina Ivanova Hannah Drescher Julia Andruszkow Thomas Longerich Jochen Nolting Roman Eickhoff D. Heise Karl P. Rheinwalt Ulf P. Neumann Florian T. Ulmer |
| author_facet | Andreas Kroh Vanina Ivanova Hannah Drescher Julia Andruszkow Thomas Longerich Jochen Nolting Roman Eickhoff D. Heise Karl P. Rheinwalt Ulf P. Neumann Florian T. Ulmer |
| author_sort | Andreas Kroh |
| collection | DOAJ |
| description | A variety of dietary nonalcoholic steatohepatitis (NASH) mouse models are available, and choosing the appropriate mouse model is one of the most important steps in the design of NASH studies. In addition to the histopathological and metabolic findings of NASH, a sufficient mouse model should guarantee a robust clinical status and good animal welfare. Three different NASH diets, a high-fat diet (HFD60), a western diet (WD), and a cafeteria diet (CAFD), were fed for 12 or 16 weeks. Metabolic assessment was conducted at baseline and before scheduled sacrifice, and liver inflammation was analyzed via fluorescence-associated cell sorting and histopathological examination. Clinical health conditions were scored weekly to assess the impact on animal welfare. The HFD60 and WD were identified as suitable NASH mouse models without a significant strain on animal welfare. Furthermore, the progression of inflammation and liver fibrosis was associated with a decreased proportion of CD3+ NK1.1+ cells. The WD represents a model of advanced-stage NASH, and the HFD60 is a strong model of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome. However, the CAFD should not be considered a NASH model. |
| format | Article |
| id | doaj-art-a4deee3eedf04716b58ade0d43f2d2f6 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-a4deee3eedf04716b58ade0d43f2d2f62025-02-03T06:00:49ZengWileyGastroenterology Research and Practice1687-61211687-630X2020-01-01202010.1155/2020/73470687347068Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal WelfareAndreas Kroh0Vanina Ivanova1Hannah Drescher2Julia Andruszkow3Thomas Longerich4Jochen Nolting5Roman Eickhoff6D. Heise7Karl P. Rheinwalt8Ulf P. Neumann9Florian T. Ulmer10Department of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of Internal Medicine III, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Pathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyInstitute of Pathology, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment for Bariatric and Metabolic Surgery, St. Franziskus-Hospital, 50825 Cologne, GermanyDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyDepartment of General, Visceral and Transplantation Surgery, RWTH Aachen University Hospital, 52074 Aachen, GermanyA variety of dietary nonalcoholic steatohepatitis (NASH) mouse models are available, and choosing the appropriate mouse model is one of the most important steps in the design of NASH studies. In addition to the histopathological and metabolic findings of NASH, a sufficient mouse model should guarantee a robust clinical status and good animal welfare. Three different NASH diets, a high-fat diet (HFD60), a western diet (WD), and a cafeteria diet (CAFD), were fed for 12 or 16 weeks. Metabolic assessment was conducted at baseline and before scheduled sacrifice, and liver inflammation was analyzed via fluorescence-associated cell sorting and histopathological examination. Clinical health conditions were scored weekly to assess the impact on animal welfare. The HFD60 and WD were identified as suitable NASH mouse models without a significant strain on animal welfare. Furthermore, the progression of inflammation and liver fibrosis was associated with a decreased proportion of CD3+ NK1.1+ cells. The WD represents a model of advanced-stage NASH, and the HFD60 is a strong model of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome. However, the CAFD should not be considered a NASH model.http://dx.doi.org/10.1155/2020/7347068 |
| spellingShingle | Andreas Kroh Vanina Ivanova Hannah Drescher Julia Andruszkow Thomas Longerich Jochen Nolting Roman Eickhoff D. Heise Karl P. Rheinwalt Ulf P. Neumann Florian T. Ulmer Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare Gastroenterology Research and Practice |
| title | Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare |
| title_full | Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare |
| title_fullStr | Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare |
| title_full_unstemmed | Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare |
| title_short | Mouse Models of Nonalcoholic Steatohepatitis: Head-to-Head Comparison of Dietary Models and Impact on Inflammation and Animal Welfare |
| title_sort | mouse models of nonalcoholic steatohepatitis head to head comparison of dietary models and impact on inflammation and animal welfare |
| url | http://dx.doi.org/10.1155/2020/7347068 |
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