Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt
Abstract Background Thaumatin-like proteins (TLPs) are crucial pathogenesis-related proteins that significantly contribute to plant defense rection. Fusarium oxysporum f. sp. cubense (Foc) causes Fusarium wilt of bananas, a serious threat to global production. However, the role of TLPs in disease re...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-06032-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585934034436096 |
|---|---|
| author | Yi-le Huo Si-wen Liu Huo-qing Huang Zai-yuan Li Mudassar Ahmad Meng-xia Zhuo Wen-juan Chen Chun-yu Li Bo Liu Ya-dong Li |
| author_facet | Yi-le Huo Si-wen Liu Huo-qing Huang Zai-yuan Li Mudassar Ahmad Meng-xia Zhuo Wen-juan Chen Chun-yu Li Bo Liu Ya-dong Li |
| author_sort | Yi-le Huo |
| collection | DOAJ |
| description | Abstract Background Thaumatin-like proteins (TLPs) are crucial pathogenesis-related proteins that significantly contribute to plant defense rection. Fusarium oxysporum f. sp. cubense (Foc) causes Fusarium wilt of bananas, a serious threat to global production. However, the role of TLPs in disease resistance remains unclear. Results This study identified 49 TLP genes in banana, predominantly localized in the extracellular space, and distributed across 11 chromosomes. The ancestor–descendant relationship was explained, six genes remained remarkably conserved across species could represent the ancestral genes of the TLP gene family. Promoter regions, transcriptome and qRT-PCR analysis suggested that MaTLP16 might be involved in disease resistance. Furthermore, transcriptional silencing of MaTLP16 resulted in more severe leaf damage compared to the control, indicating that MaTLP16 is an important Foc resistance-related gene. Conclusion This study conducted a comprehensive genome-wide identification and systematic analysis of the TLP gene family in bananas. Our findings establish a foundation for further functional studies of MaTLP genes and highlight MaTLP16 as a strong candidate for use in breeding programs aimed at enhancing resistance to Musa diseases. |
| format | Article |
| id | doaj-art-a4ce92633ca542f7befab3118779ed5b |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-a4ce92633ca542f7befab3118779ed5b2025-01-26T12:23:11ZengBMCBMC Plant Biology1471-22292025-01-0125111410.1186/s12870-024-06032-1Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wiltYi-le Huo0Si-wen Liu1Huo-qing Huang2Zai-yuan Li3Mudassar Ahmad4Meng-xia Zhuo5Wen-juan Chen6Chun-yu Li7Bo Liu8Ya-dong Li9College of Life Science, Jilin Agricultural UniversityInstitute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Academy of Agricultural SciencesInstitute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Academy of Agricultural SciencesShenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratoryof the, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen , Chinese Academy of Agricultural SciencesInstitute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Academy of Agricultural SciencesInstitute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Academy of Agricultural SciencesInstitute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Academy of Agricultural SciencesInstitute of Fruit Tree Research, Key Laboratory of South Subtropical Fruit Biology and Genetic Research Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Academy of Agricultural SciencesShenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratoryof the, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen , Chinese Academy of Agricultural SciencesCollege of Life Science, Jilin Agricultural UniversityAbstract Background Thaumatin-like proteins (TLPs) are crucial pathogenesis-related proteins that significantly contribute to plant defense rection. Fusarium oxysporum f. sp. cubense (Foc) causes Fusarium wilt of bananas, a serious threat to global production. However, the role of TLPs in disease resistance remains unclear. Results This study identified 49 TLP genes in banana, predominantly localized in the extracellular space, and distributed across 11 chromosomes. The ancestor–descendant relationship was explained, six genes remained remarkably conserved across species could represent the ancestral genes of the TLP gene family. Promoter regions, transcriptome and qRT-PCR analysis suggested that MaTLP16 might be involved in disease resistance. Furthermore, transcriptional silencing of MaTLP16 resulted in more severe leaf damage compared to the control, indicating that MaTLP16 is an important Foc resistance-related gene. Conclusion This study conducted a comprehensive genome-wide identification and systematic analysis of the TLP gene family in bananas. Our findings establish a foundation for further functional studies of MaTLP genes and highlight MaTLP16 as a strong candidate for use in breeding programs aimed at enhancing resistance to Musa diseases.https://doi.org/10.1186/s12870-024-06032-1Musa acuminataThaumatin-like proteinFocEvolutionTranscriptomicsDsRNA |
| spellingShingle | Yi-le Huo Si-wen Liu Huo-qing Huang Zai-yuan Li Mudassar Ahmad Meng-xia Zhuo Wen-juan Chen Chun-yu Li Bo Liu Ya-dong Li Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt BMC Plant Biology Musa acuminata Thaumatin-like protein Foc Evolution Transcriptomics DsRNA |
| title | Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt |
| title_full | Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt |
| title_fullStr | Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt |
| title_full_unstemmed | Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt |
| title_short | Transcriptome analysis provides insights into the role of TLP16 in Musa acuminata Resistance to Fusarium oxysporum f. sp. cubense wilt |
| title_sort | transcriptome analysis provides insights into the role of tlp16 in musa acuminata resistance to fusarium oxysporum f sp cubense wilt |
| topic | Musa acuminata Thaumatin-like protein Foc Evolution Transcriptomics DsRNA |
| url | https://doi.org/10.1186/s12870-024-06032-1 |
| work_keys_str_mv | AT yilehuo transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT siwenliu transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT huoqinghuang transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT zaiyuanli transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT mudassarahmad transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT mengxiazhuo transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT wenjuanchen transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT chunyuli transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT boliu transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt AT yadongli transcriptomeanalysisprovidesinsightsintotheroleoftlp16inmusaacuminataresistancetofusariumoxysporumfspcubensewilt |