Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer
This study was aimed at: (i) investigating the expression profiles of some antioxidant and epidermal growth factor receptor genes in cancerous and unaffected tissues of patients undergoing lung resection for non-small cell lung cancer (NSCLC) (cross-sectional phase), (ii) evaluating if gene expressi...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/147528 |
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| author | Giuseppe De Palma Paola Mozzoni Olga Acampa Eveline Internullo Paolo Carbognani Michele Rusca Matteo Goldoni Massimo Corradi Marcello Tiseo Pietro Apostoli Antonio Mutti |
| author_facet | Giuseppe De Palma Paola Mozzoni Olga Acampa Eveline Internullo Paolo Carbognani Michele Rusca Matteo Goldoni Massimo Corradi Marcello Tiseo Pietro Apostoli Antonio Mutti |
| author_sort | Giuseppe De Palma |
| collection | DOAJ |
| description | This study was aimed at: (i) investigating the expression profiles of some antioxidant and epidermal growth factor receptor genes in cancerous and unaffected tissues of patients undergoing lung resection for non-small cell lung cancer (NSCLC) (cross-sectional phase), (ii) evaluating if gene expression levels at the time of surgery may be associated to patients' survival (prospective phase).
Antioxidant genes included heme oxygenase 1 (HO-1), superoxide dismutase-1 (SOD-1), and -2 (SOD-2), whereas epidermal growth factor receptor genes consisted of epidermal growth factor receptor (EGFR) and v-erb-b2 erythroblastic leukaemia viral oncogene homolog 2 (HER-2). Twenty-eight couples of lung biopsies were obtained and gene transcripts were quantified by Real Time RT-PCR. The average follow-up of patients lasted about 60 months.
In the cancerous tissues, antioxidant genes were significantly hypo-expressed than in unaffected tissues. The HER-2 transcript levels prevailed in adenocarcinomas, whereas EGFR in squamocellular carcinomas. Patients overexpressing HER-2 in the cancerous tissues showed significantly lower 5-year survival than the others. |
| format | Article |
| id | doaj-art-a4b8c92033ca47ca8769c72a73b804c7 |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-a4b8c92033ca47ca8769c72a73b804c72025-02-03T01:27:36ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/147528147528Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung CancerGiuseppe De Palma0Paola Mozzoni1Olga Acampa2Eveline Internullo3Paolo Carbognani4Michele Rusca5Matteo Goldoni6Massimo Corradi7Marcello Tiseo8Pietro Apostoli9Antonio Mutti10Department of Experimental and Applied Medicine, Section of Occupational Health and Industrial Hygiene, University of Brescia, Piazzale Spedali Civili, 1, 25123 Brescia, ItalyISPESL Research Center at the University of Parma, via Gramsci 14, 43100 Parma, ItalyISPESL Research Center at the University of Parma, via Gramsci 14, 43100 Parma, ItalyThoracic Surgery Unit, University of Parma, via Gramsci 14, 43100 Parma, ItalyThoracic Surgery Unit, University of Parma, via Gramsci 14, 43100 Parma, ItalyThoracic Surgery Unit, University of Parma, via Gramsci 14, 43100 Parma, ItalyISPESL Research Center at the University of Parma, via Gramsci 14, 43100 Parma, ItalyLaboratory of Industrial Toxicology, Department of Clinical Medicine Nephrology and Health Sciences, University of Parma, via Gramsci 14, 43100 Parma, ItalyDivision of Medical Oncology, University of Parma, via Gramsci 14, 43100 Parma, ItalyDepartment of Experimental and Applied Medicine, Section of Occupational Health and Industrial Hygiene, University of Brescia, Piazzale Spedali Civili, 1, 25123 Brescia, ItalyLaboratory of Industrial Toxicology, Department of Clinical Medicine Nephrology and Health Sciences, University of Parma, via Gramsci 14, 43100 Parma, ItalyThis study was aimed at: (i) investigating the expression profiles of some antioxidant and epidermal growth factor receptor genes in cancerous and unaffected tissues of patients undergoing lung resection for non-small cell lung cancer (NSCLC) (cross-sectional phase), (ii) evaluating if gene expression levels at the time of surgery may be associated to patients' survival (prospective phase). Antioxidant genes included heme oxygenase 1 (HO-1), superoxide dismutase-1 (SOD-1), and -2 (SOD-2), whereas epidermal growth factor receptor genes consisted of epidermal growth factor receptor (EGFR) and v-erb-b2 erythroblastic leukaemia viral oncogene homolog 2 (HER-2). Twenty-eight couples of lung biopsies were obtained and gene transcripts were quantified by Real Time RT-PCR. The average follow-up of patients lasted about 60 months. In the cancerous tissues, antioxidant genes were significantly hypo-expressed than in unaffected tissues. The HER-2 transcript levels prevailed in adenocarcinomas, whereas EGFR in squamocellular carcinomas. Patients overexpressing HER-2 in the cancerous tissues showed significantly lower 5-year survival than the others.http://dx.doi.org/10.4061/2010/147528 |
| spellingShingle | Giuseppe De Palma Paola Mozzoni Olga Acampa Eveline Internullo Paolo Carbognani Michele Rusca Matteo Goldoni Massimo Corradi Marcello Tiseo Pietro Apostoli Antonio Mutti Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer Journal of Nucleic Acids |
| title | Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer |
| title_full | Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer |
| title_fullStr | Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer |
| title_full_unstemmed | Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer |
| title_short | Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer |
| title_sort | expression levels of some antioxidant and epidermal growth factor receptor genes in patients with early stage non small cell lung cancer |
| url | http://dx.doi.org/10.4061/2010/147528 |
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