Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents
Red currant (<i>Ribes rubrum</i> L.) berries are rich in bioactive compounds and exhibit nutritive and protective features. This research examined the content of secondary metabolites of the red currant variety Redpoll lyophilized juice and waste extract and their antioxidative and spasm...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/2/234 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587663134162944 |
|---|---|
| author | Maja Cvetković Bojana Miladinović Suzana Branković Milica Randjelović Slavoljub Živanović Nemanja Kitić Milica Milutinović Miloš Jovanović Dušica Stojanović Haris Nikšić Katarina Šavikin Dušanka Kitić |
| author_facet | Maja Cvetković Bojana Miladinović Suzana Branković Milica Randjelović Slavoljub Živanović Nemanja Kitić Milica Milutinović Miloš Jovanović Dušica Stojanović Haris Nikšić Katarina Šavikin Dušanka Kitić |
| author_sort | Maja Cvetković |
| collection | DOAJ |
| description | Red currant (<i>Ribes rubrum</i> L.) berries are rich in bioactive compounds and exhibit nutritive and protective features. This research examined the content of secondary metabolites of the red currant variety Redpoll lyophilized juice and waste extract and their antioxidative and spasmolytic effects. The flavonol and anthocyanin contents of the samples were determined using high-performance liquid chromatography. The antispasmodic effects were assessed in in vitro conditions, and the potential impact of the samples and possible action mechanisms were revealed. The results showed the prevalence of cyanidin-3-<i>O</i>-glucoside as the dominant anthocyanin with higher content in the juice sample. Quercetin content, as the prevalent flavonol, was higher in the waste sample. The berry juice showed a greater ability for scavenging free radicals, whereas the waste better inhibited lipid peroxidation. The juice was a superior antispasmodic agent for spontaneous, KCl-, CaCl<sub>2</sub>-, BaCl<sub>2</sub>-, histamine-, and acetylcholine-induced ileal contractions. This first evaluation of the red currant variety Redpoll lyophilized juice and waste extract indicated the beneficial effects of juice as an antioxidant and spasmolytic agent. Therefore, this red currant juice sample could be designated for the prevention or treatment of gastrointestinal disorders. |
| format | Article |
| id | doaj-art-a4b0172b31cf4242be499ba73d23fb5a |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-a4b0172b31cf4242be499ba73d23fb5a2025-01-24T13:46:52ZengMDPI AGPlants2223-77472025-01-0114223410.3390/plants14020234Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic AgentsMaja Cvetković0Bojana Miladinović1Suzana Branković2Milica Randjelović3Slavoljub Živanović4Nemanja Kitić5Milica Milutinović6Miloš Jovanović7Dušica Stojanović8Haris Nikšić9Katarina Šavikin10Dušanka Kitić11Department of Pharmacy, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Physiology, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaBiomedical Research Centre, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaBiomedical Research Centre, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Social Medicine and Hygiene with Medical Ecology, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaDepartment of Pharmacognosy, Faculty of Pharmacy, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaInstitute for Medicinal Plants Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Niš, Dr. Zoran Djindjić Ave 81, 18000 Niš, SerbiaRed currant (<i>Ribes rubrum</i> L.) berries are rich in bioactive compounds and exhibit nutritive and protective features. This research examined the content of secondary metabolites of the red currant variety Redpoll lyophilized juice and waste extract and their antioxidative and spasmolytic effects. The flavonol and anthocyanin contents of the samples were determined using high-performance liquid chromatography. The antispasmodic effects were assessed in in vitro conditions, and the potential impact of the samples and possible action mechanisms were revealed. The results showed the prevalence of cyanidin-3-<i>O</i>-glucoside as the dominant anthocyanin with higher content in the juice sample. Quercetin content, as the prevalent flavonol, was higher in the waste sample. The berry juice showed a greater ability for scavenging free radicals, whereas the waste better inhibited lipid peroxidation. The juice was a superior antispasmodic agent for spontaneous, KCl-, CaCl<sub>2</sub>-, BaCl<sub>2</sub>-, histamine-, and acetylcholine-induced ileal contractions. This first evaluation of the red currant variety Redpoll lyophilized juice and waste extract indicated the beneficial effects of juice as an antioxidant and spasmolytic agent. Therefore, this red currant juice sample could be designated for the prevention or treatment of gastrointestinal disorders.https://www.mdpi.com/2223-7747/14/2/234red currantsberriesjuicewasteanthocyaninsflavonols |
| spellingShingle | Maja Cvetković Bojana Miladinović Suzana Branković Milica Randjelović Slavoljub Živanović Nemanja Kitić Milica Milutinović Miloš Jovanović Dušica Stojanović Haris Nikšić Katarina Šavikin Dušanka Kitić Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents Plants red currants berries juice waste anthocyanins flavonols |
| title | Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents |
| title_full | Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents |
| title_fullStr | Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents |
| title_full_unstemmed | Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents |
| title_short | Red Currant (<i>Ribes rubrum</i> L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents |
| title_sort | red currant i ribes rubrum i l fruit waste extract and juice as potential spasmolytic agents |
| topic | red currants berries juice waste anthocyanins flavonols |
| url | https://www.mdpi.com/2223-7747/14/2/234 |
| work_keys_str_mv | AT majacvetkovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT bojanamiladinovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT suzanabrankovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT milicarandjelovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT slavoljubzivanovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT nemanjakitic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT milicamilutinovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT milosjovanovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT dusicastojanovic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT harisniksic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT katarinasavikin redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents AT dusankakitic redcurrantiribesrubrumilfruitwasteextractandjuiceaspotentialspasmolyticagents |