Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.

ABSTRACT This study aimed to obtain the pathogenicity of isolated bacteria from maribund barramundi Lates calcarifer with black body syndrome (BBS). Moribund barramundi was collected from the Center for Mariculture Development (BBPBL), Lampung Province. Five of pathogenic bacteria were found, i.e...

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Main Authors:	Akmal Izwar, Sri Nuryati, Rahman, Rini Purnomowati
Format:	Article
Language:	English
Published:	Asosiasi Sains Akuakultur Indonesia 2020-05-01
Series:	Jurnal Akuakultur Indonesia
Online Access:	https://journal.ipb.ac.id/index.php/jai/article/view/30656
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author	Akmal Izwar Sri Nuryati Rahman Rini Purnomowati
author_facet	Akmal Izwar Sri Nuryati Rahman Rini Purnomowati
author_sort	Akmal Izwar
collection	DOAJ
description	ABSTRACT This study aimed to obtain the pathogenicity of isolated bacteria from maribund barramundi Lates calcarifer with black body syndrome (BBS). Moribund barramundi was collected from the Center for Mariculture Development (BBPBL), Lampung Province. Five of pathogenic bacteria were found, i.e. Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinococcus roseus, and Pseudomonas stutzeri L02. The bacteria were tested for LD50 to obtain two types of high virulent bacteria to be used for the pathogenicity test. P. stutzeri L01 and V. harveyi were the most virulent bacteria with a bacterial density of 107 CFU/mL. Both bacteria were used for pathogenicity test with three treatments: injection of P. stutzeri L01, V. harveyi, mix P. stutzeri L01 and V. harveyi, and phosphatebuffered saline as control. Clinical symptoms showed a blackened body, bleeding under the belly, and inactive. Mortality of fish injected with P. stutzeri L01, V. harveyi and mix P. stutzeri L01 and V. harveyi was 53.33%, 55.00%, and 58.33%, respectively. Erythrocyte and hemoglobin in all treatments were not significantly different (P>0.05). However, there was significantly different in the total leukocytes of mixed P. stutzeri and V. harveyi treatment (P<0.05). In conclusion, P. stutzeri L01, V. harveyi and mix of them resulted in black body syndrome (BBS) disease. Coinfection of P. stutzeri L01 and V. harveyi produce higher mortality than single infection. Keywords: barramundi, black body syndrome, histopathology, pathogenicity ABSTRAK Penelitian ini bertujuan mendapatkan patogenisitas bakteri dari ikan kakap putih L. calcarifer yang memiliki gejala black body syndrome (BBS). Ikan diambil dari Balai Besar Pengembangan Budidaya Laut (BBPBL), Lampung. Lima jenis bakteri patogen ditemukan, yaitu Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinococcus roseus, dan Pseudomonas stutzeri L02. Bakteri diuji LD50 untuk mendapatkan dua jenis bakteri virulen tinggi yang akan digunakan untuk uji patogenisitas. P. stutzeri L01 dan V. harveyi adalah bakteri paling virulen dengan kepadatan bakteri 107 CFU/mL. Uji patogenisitas dengan tiga perlakuan: injeksi P. stutzeri L01, V. harveyi, campuran P. stutzeri L01 dan V. harveyi, serta phosphate-buffered saline sebagai kontrol. Gejala klinis menunjukkan tubuh menghitam, perdarahan di bawah perut, dan pergerakan tidak aktif. Mortalitas ikan yang disuntik dengan P. stutzeri L01, V. harveyi dan campuran P. stutzeri L01 dan V. harveyi masing-masing sebesar 53,33%, 55,00%, dan 58,33%. Eritrosit dan hemoglobin pada semua perlakuan tidak berbeda nyata (P>0,05). Namun, ada perbedaan yang signifikan dalam total leukosit perlakuan campuran P. stutzeri L01 dan V. harveyi (P<0,05). P. stutzeri L01, V. harveyi dan campuran P. stutzeri L01, V. harveyi dapat menyebabkan black body syndrome (BBS). Koinfeksi P. stutzeri L01 dan V. harveyi menghasilkan mortalitas lebih tinggi daripada infeksi tunggal. Kata kunci: barramundi, black body syndrome, histopatologi, patogenisitas
format	Article
id	doaj-art-a47e695233f548ed968a0527d132239e
institution	Kabale University
issn	1412-5269 2354-6700
language	English
publishDate	2020-05-01
publisher	Asosiasi Sains Akuakultur Indonesia
record_format	Article
series	Jurnal Akuakultur Indonesia
spelling	doaj-art-a47e695233f548ed968a0527d132239e2025-01-24T17:56:53ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002020-05-0119110.19027/jai.19.1.39-4930656Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.Akmal Izwar0Sri Nuryati1Rahman2Rini Purnomowati3Aquaculture Science, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University , Dramaga, West Java, Indonesia 16680Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680Centre for Mariculture Research Development, Lampung ABSTRACT This study aimed to obtain the pathogenicity of isolated bacteria from maribund barramundi Lates calcarifer with black body syndrome (BBS). Moribund barramundi was collected from the Center for Mariculture Development (BBPBL), Lampung Province. Five of pathogenic bacteria were found, i.e. Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinococcus roseus, and Pseudomonas stutzeri L02. The bacteria were tested for LD50 to obtain two types of high virulent bacteria to be used for the pathogenicity test. P. stutzeri L01 and V. harveyi were the most virulent bacteria with a bacterial density of 107 CFU/mL. Both bacteria were used for pathogenicity test with three treatments: injection of P. stutzeri L01, V. harveyi, mix P. stutzeri L01 and V. harveyi, and phosphatebuffered saline as control. Clinical symptoms showed a blackened body, bleeding under the belly, and inactive. Mortality of fish injected with P. stutzeri L01, V. harveyi and mix P. stutzeri L01 and V. harveyi was 53.33%, 55.00%, and 58.33%, respectively. Erythrocyte and hemoglobin in all treatments were not significantly different (P>0.05). However, there was significantly different in the total leukocytes of mixed P. stutzeri and V. harveyi treatment (P<0.05). In conclusion, P. stutzeri L01, V. harveyi and mix of them resulted in black body syndrome (BBS) disease. Coinfection of P. stutzeri L01 and V. harveyi produce higher mortality than single infection. Keywords: barramundi, black body syndrome, histopathology, pathogenicity ABSTRAK Penelitian ini bertujuan mendapatkan patogenisitas bakteri dari ikan kakap putih L. calcarifer yang memiliki gejala black body syndrome (BBS). Ikan diambil dari Balai Besar Pengembangan Budidaya Laut (BBPBL), Lampung. Lima jenis bakteri patogen ditemukan, yaitu Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinococcus roseus, dan Pseudomonas stutzeri L02. Bakteri diuji LD50 untuk mendapatkan dua jenis bakteri virulen tinggi yang akan digunakan untuk uji patogenisitas. P. stutzeri L01 dan V. harveyi adalah bakteri paling virulen dengan kepadatan bakteri 107 CFU/mL. Uji patogenisitas dengan tiga perlakuan: injeksi P. stutzeri L01, V. harveyi, campuran P. stutzeri L01 dan V. harveyi, serta phosphate-buffered saline sebagai kontrol. Gejala klinis menunjukkan tubuh menghitam, perdarahan di bawah perut, dan pergerakan tidak aktif. Mortalitas ikan yang disuntik dengan P. stutzeri L01, V. harveyi dan campuran P. stutzeri L01 dan V. harveyi masing-masing sebesar 53,33%, 55,00%, dan 58,33%. Eritrosit dan hemoglobin pada semua perlakuan tidak berbeda nyata (P>0,05). Namun, ada perbedaan yang signifikan dalam total leukosit perlakuan campuran P. stutzeri L01 dan V. harveyi (P<0,05). P. stutzeri L01, V. harveyi dan campuran P. stutzeri L01, V. harveyi dapat menyebabkan black body syndrome (BBS). Koinfeksi P. stutzeri L01 dan V. harveyi menghasilkan mortalitas lebih tinggi daripada infeksi tunggal. Kata kunci: barramundi, black body syndrome, histopatologi, patogenisitas https://journal.ipb.ac.id/index.php/jai/article/view/30656
spellingShingle	Akmal Izwar Sri Nuryati Rahman Rini Purnomowati Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B. Jurnal Akuakultur Indonesia
title	Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.
title_full	Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.
title_fullStr	Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.
title_full_unstemmed	Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.
title_short	Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.
title_sort	isolation identification and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi lates calcarifer b
url	https://journal.ipb.ac.id/index.php/jai/article/view/30656
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Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B.

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