Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment
There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines—c...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/817498 |
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| author | Antonio Brú Juan-Carlos Souto Sonia Alcolea Rosa Antón Angel Remacha Mercedes Camacho Marta Soler Isabel Brú Amelia Porres Luis Vila |
| author_facet | Antonio Brú Juan-Carlos Souto Sonia Alcolea Rosa Antón Angel Remacha Mercedes Camacho Marta Soler Isabel Brú Amelia Porres Luis Vila |
| author_sort | Antonio Brú |
| collection | DOAJ |
| description | There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines—colorectal adenocarcinoma (HT-29) and pharyngeal squamous-cell carcinoma (FaDu) cells—to act as inflammatory foci. PMNs were cytotoxic to both lines, the adhesion of the PMNs to the tumour cells being important in this effect. The tumour cells released appreciable amounts of IL-8 and GROα, and induced the transmigration of PMN through human microvascular-EC monolayers. Conditioning media associated with both lines induced the adhesion of PMN and the surface expression of ICAM-1 in microvascular-EC. In addition, FaDu-conditioning-medium strongly induced the production of proinflammatory cytokines by microvascular-EC. These results support the idea that tumour cells might normally induce a potent acute inflammatory response, leading to their own
destruction. |
| format | Article |
| id | doaj-art-a47c5eb13fcc465ca7242245a98cc934 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a47c5eb13fcc465ca7242245a98cc9342025-02-03T01:21:40ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/817498817498Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour TreatmentAntonio Brú0Juan-Carlos Souto1Sonia Alcolea2Rosa Antón3Angel Remacha4Mercedes Camacho5Marta Soler6Isabel Brú7Amelia Porres8Luis Vila9Applied Mathematics Department, Universidad Complutense de Madrid, 28040 Madrid, SpainHaematology Department, Institute of Research of Hospital Santa Creu i Sant Pau, 08025 Barcelona, SpainLaboratory of Angiology, Vascular Biology and Inflammation, Institute of Research of Hospital Santa Creu i Sant Pau, 08025 Barcelona, SpainLaboratory of Angiology, Vascular Biology and Inflammation, Institute of Research of Hospital Santa Creu i Sant Pau, 08025 Barcelona, SpainHaematology Department, Complejo Hospitalario Virgen de la Salud, 45004 Toledo, SpainLaboratory of Angiology, Vascular Biology and Inflammation, Institute of Research of Hospital Santa Creu i Sant Pau, 08025 Barcelona, SpainLaboratory of Angiology, Vascular Biology and Inflammation, Institute of Research of Hospital Santa Creu i Sant Pau, 08025 Barcelona, SpainCentro de Salud La Estación, Talavera de la Reina, 45600 Toledo, SpainResearch Laboratory, Fundación Jiménez Díaz, 28040 Madrid, SpainLaboratory of Angiology, Vascular Biology and Inflammation, Institute of Research of Hospital Santa Creu i Sant Pau, 08025 Barcelona, SpainThere is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines—colorectal adenocarcinoma (HT-29) and pharyngeal squamous-cell carcinoma (FaDu) cells—to act as inflammatory foci. PMNs were cytotoxic to both lines, the adhesion of the PMNs to the tumour cells being important in this effect. The tumour cells released appreciable amounts of IL-8 and GROα, and induced the transmigration of PMN through human microvascular-EC monolayers. Conditioning media associated with both lines induced the adhesion of PMN and the surface expression of ICAM-1 in microvascular-EC. In addition, FaDu-conditioning-medium strongly induced the production of proinflammatory cytokines by microvascular-EC. These results support the idea that tumour cells might normally induce a potent acute inflammatory response, leading to their own destruction.http://dx.doi.org/10.1155/2009/817498 |
| spellingShingle | Antonio Brú Juan-Carlos Souto Sonia Alcolea Rosa Antón Angel Remacha Mercedes Camacho Marta Soler Isabel Brú Amelia Porres Luis Vila Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment Mediators of Inflammation |
| title | Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment |
| title_full | Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment |
| title_fullStr | Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment |
| title_full_unstemmed | Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment |
| title_short | Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment |
| title_sort | tumour cell lines ht 29 and fadu produce proinflammatory cytokines and activate neutrophils in vitro possible applications for neutrophil based antitumour treatment |
| url | http://dx.doi.org/10.1155/2009/817498 |
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