Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model
Objective. Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/809801 |
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| author | Gonca Cayir Keles Umut Balli Burcu Ozkan Cetinkaya Bulent Ayas Arzu Findik Zeynep Pinar Keles Ferda Pamuk |
| author_facet | Gonca Cayir Keles Umut Balli Burcu Ozkan Cetinkaya Bulent Ayas Arzu Findik Zeynep Pinar Keles Ferda Pamuk |
| author_sort | Gonca Cayir Keles |
| collection | DOAJ |
| description | Objective. Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis. Methods. Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method. Results. Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 (P<0.01). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups (P>0.05). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups (P<0.001). Conclusion. PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings. |
| format | Article |
| id | doaj-art-a471289a3cbc4e3a89c2813679bf44d5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a471289a3cbc4e3a89c2813679bf44d52025-02-03T06:42:16ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/809801809801Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis ModelGonca Cayir Keles0Umut Balli1Burcu Ozkan Cetinkaya2Bulent Ayas3Arzu Findik4Zeynep Pinar Keles5Ferda Pamuk6Department of Periodontology, Faculty of Dentistry, Ondokuzmayis University, 55139 Samsun, TurkeyDepartment of Periodontology, Faculty of Dentistry, Ondokuzmayis University, 55139 Samsun, TurkeyDepartment of Periodontology, Faculty of Dentistry, Ondokuzmayis University, 55139 Samsun, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Ondokuzmayis University, 55139 Samsun, TurkeyDepartment of Microbiology, Faculty of Veterinary Medicine, Ondokuzmayis University, 55139 Samsun, TurkeyDepartment of Periodontology, Faculty of Dentistry, Ondokuzmayis University, 55139 Samsun, TurkeyDepartment of Periodontology, Faculty of Dentistry, Istanbul Aydin University, 34380 Istanbul, TurkeyObjective. Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis. Methods. Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method. Results. Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 (P<0.01). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups (P>0.05). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups (P<0.001). Conclusion. PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings.http://dx.doi.org/10.1155/2012/809801 |
| spellingShingle | Gonca Cayir Keles Umut Balli Burcu Ozkan Cetinkaya Bulent Ayas Arzu Findik Zeynep Pinar Keles Ferda Pamuk Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model Mediators of Inflammation |
| title | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model |
| title_full | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model |
| title_fullStr | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model |
| title_full_unstemmed | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model |
| title_short | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model |
| title_sort | biochemical analysis of pentraxin 3 and fibrinogen levels in experimental periodontitis model |
| url | http://dx.doi.org/10.1155/2012/809801 |
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