Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress
Cryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress.. Combining PacBio Iso Sequencing and Illumina RNA Sequencing, 10,508 full-length transcripts were obtained, with 9,523 successfully...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2025-01-01
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| Series: | Liang you shipin ke-ji |
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| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250127 |
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| author | WU Tian-yi WU Yi FANG Zhi-yi ZHANG Xiao-pei WANG Fu-ling XUE Ding-rong |
| author_facet | WU Tian-yi WU Yi FANG Zhi-yi ZHANG Xiao-pei WANG Fu-ling XUE Ding-rong |
| author_sort | WU Tian-yi |
| collection | DOAJ |
| description | Cryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress.. Combining PacBio Iso Sequencing and Illumina RNA Sequencing, 10,508 full-length transcripts were obtained, with 9,523 successfully annotated. Compared to the control group, 477 genes exhibited significant differential expressed under low-temperature stress, including 177 upregulated and 300 downregulated genes. KEGG analysis revealed that differentially expressed genes were primarily enriched in metabolic pathways, fatty acid biosynthesis, and secondary metabolite biosynthesis. RT-qPCR was used to analyze the expression levels of six differentially expressed genes: heat shock protein, fatty acid synthase, chitinase, trehalose transporter, cytochrome P450, and vitellogenin.. The results demonstrated consistency between RT-qPCR and transcriptome sequencing analysis. This study offers preliminary insights into the molecular mechanisms of C. ferrugineus adaptation to low-temperature stress, forming a foundation for applying low-temperature grain storage technology in integrated pest management strategies. |
| format | Article |
| id | doaj-art-a4629326eda54447b356113a425bf5ee |
| institution | Kabale University |
| issn | 1007-7561 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj-art-a4629326eda54447b356113a425bf5ee2025-01-23T13:59:01ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612025-01-0133123824510.16210/j.cnki.1007-7561.2025.01.026Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature StressWU Tian-yi0WU Yi1FANG Zhi-yi2ZHANG Xiao-pei3WANG Fu-ling4XUE Ding-rong5National Engineering Research Centre of Grain Storage and Logistics, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaNational Engineering Research Centre of Grain Storage and Logistics, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaFujian Grain Reserves Co., Ltd. Zhangpu Depot, Zhangzhou, Fujian 363200, ChinaGuangxi Zhuang Autonomous Region Liuzhou Grain Reserves Co., Ltd., Liuzhou, Guangxi 545007, ChinaGuangxi Zhuang Autonomous Region Liuzhou Grain Reserves Co., Ltd., Liuzhou, Guangxi 545007, ChinaNational Engineering Research Centre of Grain Storage and Logistics, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaCryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress.. Combining PacBio Iso Sequencing and Illumina RNA Sequencing, 10,508 full-length transcripts were obtained, with 9,523 successfully annotated. Compared to the control group, 477 genes exhibited significant differential expressed under low-temperature stress, including 177 upregulated and 300 downregulated genes. KEGG analysis revealed that differentially expressed genes were primarily enriched in metabolic pathways, fatty acid biosynthesis, and secondary metabolite biosynthesis. RT-qPCR was used to analyze the expression levels of six differentially expressed genes: heat shock protein, fatty acid synthase, chitinase, trehalose transporter, cytochrome P450, and vitellogenin.. The results demonstrated consistency between RT-qPCR and transcriptome sequencing analysis. This study offers preliminary insights into the molecular mechanisms of C. ferrugineus adaptation to low-temperature stress, forming a foundation for applying low-temperature grain storage technology in integrated pest management strategies.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250127cryptolestes ferrugineustranscriptome analysislow temperature stressdifferentially expressed genesrt-qpcr |
| spellingShingle | WU Tian-yi WU Yi FANG Zhi-yi ZHANG Xiao-pei WANG Fu-ling XUE Ding-rong Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress Liang you shipin ke-ji cryptolestes ferrugineus transcriptome analysis low temperature stress differentially expressed genes rt-qpcr |
| title | Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress |
| title_full | Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress |
| title_fullStr | Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress |
| title_full_unstemmed | Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress |
| title_short | Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress |
| title_sort | transcriptome analysis of cryptolestes ferrugineus under low temperature stress |
| topic | cryptolestes ferrugineus transcriptome analysis low temperature stress differentially expressed genes rt-qpcr |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250127 |
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