Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress
Cryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress.. Combining PacBio Iso Sequencing and Illumina RNA Sequencing, 10,508 full-length transcripts were obtained, with 9,523 successfully...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academy of National Food and Strategic Reserves Administration
2025-01-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250127 |
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| Summary: | Cryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress.. Combining PacBio Iso Sequencing and Illumina RNA Sequencing, 10,508 full-length transcripts were obtained, with 9,523 successfully annotated. Compared to the control group, 477 genes exhibited significant differential expressed under low-temperature stress, including 177 upregulated and 300 downregulated genes. KEGG analysis revealed that differentially expressed genes were primarily enriched in metabolic pathways, fatty acid biosynthesis, and secondary metabolite biosynthesis. RT-qPCR was used to analyze the expression levels of six differentially expressed genes: heat shock protein, fatty acid synthase, chitinase, trehalose transporter, cytochrome P450, and vitellogenin.. The results demonstrated consistency between RT-qPCR and transcriptome sequencing analysis. This study offers preliminary insights into the molecular mechanisms of C. ferrugineus adaptation to low-temperature stress, forming a foundation for applying low-temperature grain storage technology in integrated pest management strategies. |
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| ISSN: | 1007-7561 |