xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.

Prostate cancer is the second most common type of cancer in male worldwide. Stromal-epithelial interaction is thought to have a major impact on cancer development and progression. Previous studies have shown that interaction via soluble factors lead to a reduction in the expression of xCT and AL1220...

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Main Authors: Elena D Wilhelm, Jaroslaw T Dankert, Marc Wiesehöfer, Sven Wach, Mathias Wagner, Martin Spahn, Marianna Kruithof-de Julio, Gunther Wennemuth
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0318213
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author Elena D Wilhelm
Jaroslaw T Dankert
Marc Wiesehöfer
Sven Wach
Mathias Wagner
Martin Spahn
Marianna Kruithof-de Julio
Gunther Wennemuth
author_facet Elena D Wilhelm
Jaroslaw T Dankert
Marc Wiesehöfer
Sven Wach
Mathias Wagner
Martin Spahn
Marianna Kruithof-de Julio
Gunther Wennemuth
author_sort Elena D Wilhelm
collection DOAJ
description Prostate cancer is the second most common type of cancer in male worldwide. Stromal-epithelial interaction is thought to have a major impact on cancer development and progression. Previous studies have shown that interaction via soluble factors lead to a reduction in the expression of xCT and AL122023.1 in the prostate carcinoma cell line LNCaP after seven days of co-culture with primary stromal p21 cells. In this study, we validated the repression of xCT and AL122023.1 at RNA level using quantitative real-time PCR and at protein level by Western Blotting. Furthermore, xCT is known to be a putative target for miRNAs miR-26a, miR-30d and miR-30e, which in turn potentially interact with AL122023.1. The lncRNA-miRNA-interaction was verified by luciferase reporter assays. However, miR-26a/-30d/-30e did not inhibit xCT expression at protein level. Nevertheless, indirect inhibitory effect of AL122023.1 on the xCT expression could be shown. Moreover, immunostaining revealed precise xCT expression in neuroendocrine cells, ranging from fetal, healthy juvenile, and adult prostate tissue to benign prostatic hyperplasia and finally advanced prostate cancer. This study explores the relevance and function of xCT and AL122023.1 in the prostate and exposes xCT as a potential marker or therapeutic target in high-risk prostate cancer.
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spelling doaj-art-a45f0995db4b45438e35899547e4c22c2025-02-05T05:32:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031821310.1371/journal.pone.0318213xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.Elena D WilhelmJaroslaw T DankertMarc WiesehöferSven WachMathias WagnerMartin SpahnMarianna Kruithof-de JulioGunther WennemuthProstate cancer is the second most common type of cancer in male worldwide. Stromal-epithelial interaction is thought to have a major impact on cancer development and progression. Previous studies have shown that interaction via soluble factors lead to a reduction in the expression of xCT and AL122023.1 in the prostate carcinoma cell line LNCaP after seven days of co-culture with primary stromal p21 cells. In this study, we validated the repression of xCT and AL122023.1 at RNA level using quantitative real-time PCR and at protein level by Western Blotting. Furthermore, xCT is known to be a putative target for miRNAs miR-26a, miR-30d and miR-30e, which in turn potentially interact with AL122023.1. The lncRNA-miRNA-interaction was verified by luciferase reporter assays. However, miR-26a/-30d/-30e did not inhibit xCT expression at protein level. Nevertheless, indirect inhibitory effect of AL122023.1 on the xCT expression could be shown. Moreover, immunostaining revealed precise xCT expression in neuroendocrine cells, ranging from fetal, healthy juvenile, and adult prostate tissue to benign prostatic hyperplasia and finally advanced prostate cancer. This study explores the relevance and function of xCT and AL122023.1 in the prostate and exposes xCT as a potential marker or therapeutic target in high-risk prostate cancer.https://doi.org/10.1371/journal.pone.0318213
spellingShingle Elena D Wilhelm
Jaroslaw T Dankert
Marc Wiesehöfer
Sven Wach
Mathias Wagner
Martin Spahn
Marianna Kruithof-de Julio
Gunther Wennemuth
xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.
PLoS ONE
title xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.
title_full xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.
title_fullStr xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.
title_full_unstemmed xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.
title_short xCT as a potential marker for neuroendocrine cells in high-risk prostate cancer and the relation to AL122023.1-miR-26a/30d/30e axis.
title_sort xct as a potential marker for neuroendocrine cells in high risk prostate cancer and the relation to al122023 1 mir 26a 30d 30e axis
url https://doi.org/10.1371/journal.pone.0318213
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