Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites
Solid-state fermentation (SSF) can increase the nutritional quality of low value substrates for insects. In this study, SSF using different fungal species was applied on a hemp waste substrate, and the fermentation was followed by a black soldier fly larvae (BSFL) feeding experiment during which 300...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1449233/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589861379375104 |
|---|---|
| author | Wael Yakti Nadja Förster Marcus Müller Sebastian Beck Simon Schulz Inga Mewis Christian Ulrichs |
| author_facet | Wael Yakti Nadja Förster Marcus Müller Sebastian Beck Simon Schulz Inga Mewis Christian Ulrichs |
| author_sort | Wael Yakti |
| collection | DOAJ |
| description | Solid-state fermentation (SSF) can increase the nutritional quality of low value substrates for insects. In this study, SSF using different fungal species was applied on a hemp waste substrate, and the fermentation was followed by a black soldier fly larvae (BSFL) feeding experiment during which 300 larvae were grown on 200 g (20.1% DM) substrate for 7–9 days depending on the treatment. Besides assessing the BSFL performance parameters, the presence of hemp cannabinoids, flavonoids, and terpenes was assessed through the process and compared among the treatments. The results show that BSFL growth parameters varied depending on the fungal species used. Fermenting the substrate with Ganoderma lucidum can lead to an increase in the BSFL dry yield (4.54 g) compared to the untreated substrate (2.86 g), likely due to enhancing carbon accessibility in the substrate. SSF using Trichoderma reesei increased the cannabidiol and ∆9-tetrahydrocannabinol mass fractions in the substrate, and consequently in the produced BSFL biomass, while decreasing the amounts of acidic cannabinoids. Both Hypsizygus ulmarius and Pleurotus ostreatus effectively removed cannabinoids from the substrate. This study confirms that pre-treating hemp wastes via SSF can enhance their nutritional value and/or reduce bioactive secondary metabolites, with different fungal species offering different and complementary performances in achieving different biotechnological goals. |
| format | Article |
| id | doaj-art-a41e40f605ac465c8e84db60c7728d47 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-a41e40f605ac465c8e84db60c7728d472025-01-24T07:13:28ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011310.3389/fbioe.2025.14492331449233Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolitesWael Yakti0Nadja Förster1Marcus Müller2Sebastian Beck3Simon Schulz4Inga Mewis5Christian Ulrichs6Faculty of Life Sciences, Thaer-Institute of Agricultural and Horticultural Sciences, Urban Plant Ecophysiology Division, Humboldt-Universität zu Berlin, Berlin, GermanyFaculty of Life Sciences, Thaer-Institute of Agricultural and Horticultural Sciences, Urban Plant Ecophysiology Division, Humboldt-Universität zu Berlin, Berlin, GermanyFaculty of Life Sciences, Thaer-Institute of Agricultural and Horticultural Sciences, Urban Plant Ecophysiology Division, Humboldt-Universität zu Berlin, Berlin, GermanyDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Applied Analytical and Environmental Chemistry, Humboldt-Universität zu Berlin, Berlin, GermanyFaculty of Life Sciences, Thaer-Institute of Agricultural and Horticultural Sciences, Urban Plant Ecophysiology Division, Humboldt-Universität zu Berlin, Berlin, GermanyFaculty of Life Sciences, Thaer-Institute of Agricultural and Horticultural Sciences, Urban Plant Ecophysiology Division, Humboldt-Universität zu Berlin, Berlin, GermanyFaculty of Life Sciences, Thaer-Institute of Agricultural and Horticultural Sciences, Urban Plant Ecophysiology Division, Humboldt-Universität zu Berlin, Berlin, GermanySolid-state fermentation (SSF) can increase the nutritional quality of low value substrates for insects. In this study, SSF using different fungal species was applied on a hemp waste substrate, and the fermentation was followed by a black soldier fly larvae (BSFL) feeding experiment during which 300 larvae were grown on 200 g (20.1% DM) substrate for 7–9 days depending on the treatment. Besides assessing the BSFL performance parameters, the presence of hemp cannabinoids, flavonoids, and terpenes was assessed through the process and compared among the treatments. The results show that BSFL growth parameters varied depending on the fungal species used. Fermenting the substrate with Ganoderma lucidum can lead to an increase in the BSFL dry yield (4.54 g) compared to the untreated substrate (2.86 g), likely due to enhancing carbon accessibility in the substrate. SSF using Trichoderma reesei increased the cannabidiol and ∆9-tetrahydrocannabinol mass fractions in the substrate, and consequently in the produced BSFL biomass, while decreasing the amounts of acidic cannabinoids. Both Hypsizygus ulmarius and Pleurotus ostreatus effectively removed cannabinoids from the substrate. This study confirms that pre-treating hemp wastes via SSF can enhance their nutritional value and/or reduce bioactive secondary metabolites, with different fungal species offering different and complementary performances in achieving different biotechnological goals.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1449233/fullblack soldier flycannabisplant secondary metabolitessolid-state fermentationinsect protein |
| spellingShingle | Wael Yakti Nadja Förster Marcus Müller Sebastian Beck Simon Schulz Inga Mewis Christian Ulrichs Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites Frontiers in Bioengineering and Biotechnology black soldier fly cannabis plant secondary metabolites solid-state fermentation insect protein |
| title | Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites |
| title_full | Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites |
| title_fullStr | Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites |
| title_full_unstemmed | Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites |
| title_short | Solid-state fermentation of hemp waste: enhancing the performance of Hermetia illucens larvae and altering the composition of hemp secondary metabolites |
| title_sort | solid state fermentation of hemp waste enhancing the performance of hermetia illucens larvae and altering the composition of hemp secondary metabolites |
| topic | black soldier fly cannabis plant secondary metabolites solid-state fermentation insect protein |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1449233/full |
| work_keys_str_mv | AT waelyakti solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites AT nadjaforster solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites AT marcusmuller solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites AT sebastianbeck solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites AT simonschulz solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites AT ingamewis solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites AT christianulrichs solidstatefermentationofhempwasteenhancingtheperformanceofhermetiaillucenslarvaeandalteringthecompositionofhempsecondarymetabolites |