Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET
Abstract Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the analytical methods useful for monitoring enzymatic PET-degradation. For the HPLC-based assessment, the incorporation of a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-84177-7 |
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| author | Krisztina Boros Blanka Eszter Nagy Raluca Bianca Tomoiagă Róbert Tőtős Monica Ioana Toșa Csaba Paizs László Csaba Bencze |
| author_facet | Krisztina Boros Blanka Eszter Nagy Raluca Bianca Tomoiagă Róbert Tőtős Monica Ioana Toșa Csaba Paizs László Csaba Bencze |
| author_sort | Krisztina Boros |
| collection | DOAJ |
| description | Abstract Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the analytical methods useful for monitoring enzymatic PET-degradation. For the HPLC-based assessment, the incorporation of an internal standard within the analytic procedure enabled a more accurate quantification of the overall TPA content and the assessment of molar distributions and relative content of each aromatic degradation product. The provided calibration curves cover a broad concentration range, from µM to low mM scale, facilitating assessment of both lower and higher PETase activities, with a limit of detection positioned below the reported PET-degrading activities. The increased reproducibility and accuracy of the improved HPLC method, compared to the previous methods, was supported by lower dispersion of product concentrations and their lower deviation from theoretical values over multiple measurements. The other predominantly employed UV-spectroscopy assay was also improved in terms of employed wavelength and medium extinction coefficient of the three aromatic degradation products, while being cross-validated by the improved HPLC method. Finally, both methods were used for monitoring the product formation within the leaf-branch compost cutinase (LCC)-mediated PET-hydrolysis and provided individual time-productivity profiles for each aromatic degradation product. |
| format | Article |
| id | doaj-art-a40b4c1eedde4432afd3038e7fa227dc |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a40b4c1eedde4432afd3038e7fa227dc2025-01-19T12:21:24ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-024-84177-7Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PETKrisztina Boros0Blanka Eszter Nagy1Raluca Bianca Tomoiagă2Róbert Tőtős3Monica Ioana Toșa4Csaba Paizs5László Csaba Bencze6Enzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityEnzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityEnzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityEnzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityEnzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityEnzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityEnzymology and Applied Biocatalysis Research Center, Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai UniversityAbstract Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the analytical methods useful for monitoring enzymatic PET-degradation. For the HPLC-based assessment, the incorporation of an internal standard within the analytic procedure enabled a more accurate quantification of the overall TPA content and the assessment of molar distributions and relative content of each aromatic degradation product. The provided calibration curves cover a broad concentration range, from µM to low mM scale, facilitating assessment of both lower and higher PETase activities, with a limit of detection positioned below the reported PET-degrading activities. The increased reproducibility and accuracy of the improved HPLC method, compared to the previous methods, was supported by lower dispersion of product concentrations and their lower deviation from theoretical values over multiple measurements. The other predominantly employed UV-spectroscopy assay was also improved in terms of employed wavelength and medium extinction coefficient of the three aromatic degradation products, while being cross-validated by the improved HPLC method. Finally, both methods were used for monitoring the product formation within the leaf-branch compost cutinase (LCC)-mediated PET-hydrolysis and provided individual time-productivity profiles for each aromatic degradation product.https://doi.org/10.1038/s41598-024-84177-7 |
| spellingShingle | Krisztina Boros Blanka Eszter Nagy Raluca Bianca Tomoiagă Róbert Tőtős Monica Ioana Toșa Csaba Paizs László Csaba Bencze Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET Scientific Reports |
| title | Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET |
| title_full | Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET |
| title_fullStr | Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET |
| title_full_unstemmed | Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET |
| title_short | Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET |
| title_sort | fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of pet |
| url | https://doi.org/10.1038/s41598-024-84177-7 |
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