Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model
In a swine model of ischemia/reperfusion injury coupled with sepsis, we have previously shown attenuation of secondary organ injury and decreased mortality with negative pressure therapy (NPT). We hypothesized that NPT modulates the intestinal microenvironment by mediating the innate immune system....
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/419841 |
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| author | Kenneth C. Norbury Mary Pat Moyer |
| author_facet | Kenneth C. Norbury Mary Pat Moyer |
| author_sort | Kenneth C. Norbury |
| collection | DOAJ |
| description | In a swine model of ischemia/reperfusion injury coupled with sepsis, we have previously shown attenuation of secondary organ injury and decreased mortality with negative pressure therapy (NPT). We hypothesized that NPT modulates the intestinal microenvironment by mediating the innate immune system. Sepsis was induced in 12 anesthetized female pigs. Group 1 (n=6) was decompressed at 12 hrs after injury (T12) and treated with standard of care (SOC), and group 2 (n=6) with NPT for up to T48. Immunoparalysis was evident as lymphocytopenia at T24 in both groups; however, survival was improved in the NPT group versus SOC (Odds ratio = 4.0). The SOC group showed significant reduction in lymphocyte numbers compared to NPT group by T48 (p<0.05). The capacity of peritoneal fluid to stimulate a robust reactive oxygen species response in vitro was greater for the NPT group, peaking at T24 for both M1 (p=0.0197) and M2 macrophages (p=0.085). Plasma elicited little if any effect which was confirmed by microarray analysis. In this septic swine model NPT appeared to modulate the intestinal microenvironment, facilitating an early robust, yet transient, host defense mediated by M1 and M2 macrophages. NPT may help overcome immunoparalysis that occurs during inflammatory response to septic injury. |
| format | Article |
| id | doaj-art-a3fbf209b3c0492e89fffb5a11149040 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-a3fbf209b3c0492e89fffb5a111490402025-02-03T06:05:08ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/419841419841Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic ModelKenneth C. Norbury0Mary Pat Moyer1KCI, An Acelity Company, San Antonio, TX 78249, USAINCELL Corporation LLC, San Antonio, TX 78249, USAIn a swine model of ischemia/reperfusion injury coupled with sepsis, we have previously shown attenuation of secondary organ injury and decreased mortality with negative pressure therapy (NPT). We hypothesized that NPT modulates the intestinal microenvironment by mediating the innate immune system. Sepsis was induced in 12 anesthetized female pigs. Group 1 (n=6) was decompressed at 12 hrs after injury (T12) and treated with standard of care (SOC), and group 2 (n=6) with NPT for up to T48. Immunoparalysis was evident as lymphocytopenia at T24 in both groups; however, survival was improved in the NPT group versus SOC (Odds ratio = 4.0). The SOC group showed significant reduction in lymphocyte numbers compared to NPT group by T48 (p<0.05). The capacity of peritoneal fluid to stimulate a robust reactive oxygen species response in vitro was greater for the NPT group, peaking at T24 for both M1 (p=0.0197) and M2 macrophages (p=0.085). Plasma elicited little if any effect which was confirmed by microarray analysis. In this septic swine model NPT appeared to modulate the intestinal microenvironment, facilitating an early robust, yet transient, host defense mediated by M1 and M2 macrophages. NPT may help overcome immunoparalysis that occurs during inflammatory response to septic injury.http://dx.doi.org/10.1155/2015/419841 |
| spellingShingle | Kenneth C. Norbury Mary Pat Moyer Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model Mediators of Inflammation |
| title | Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model |
| title_full | Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model |
| title_fullStr | Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model |
| title_full_unstemmed | Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model |
| title_short | Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model |
| title_sort | effect of negative pressure therapy on the inflammatory response of the intestinal microenvironment in a porcine septic model |
| url | http://dx.doi.org/10.1155/2015/419841 |
| work_keys_str_mv | AT kennethcnorbury effectofnegativepressuretherapyontheinflammatoryresponseoftheintestinalmicroenvironmentinaporcinesepticmodel AT marypatmoyer effectofnegativepressuretherapyontheinflammatoryresponseoftheintestinalmicroenvironmentinaporcinesepticmodel |