Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes
Antimicrobial resistance is a serious global public health concern that could endanger the efficacy of antibiotics used for the treatment of diseases in humans, animals, and plants. The objective of this study was to characterize the antimicrobial resistance (AMR) pattern of Salmonella strains isola...
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Elsevier
2025-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000926 |
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| author | Yagya Adhikari Dianna V. Bourassa Sabin Poudel Matthew A. Bailey Richard J. Buhr Kenneth S. Macklin |
| author_facet | Yagya Adhikari Dianna V. Bourassa Sabin Poudel Matthew A. Bailey Richard J. Buhr Kenneth S. Macklin |
| author_sort | Yagya Adhikari |
| collection | DOAJ |
| description | Antimicrobial resistance is a serious global public health concern that could endanger the efficacy of antibiotics used for the treatment of diseases in humans, animals, and plants. The objective of this study was to characterize the antimicrobial resistance (AMR) pattern of Salmonella strains isolated from different stages of both No-Antibiotics-Ever (NAE) and conventional integrated broiler complexes. ResFinder was used to identify AMR genes from whole genome sequences while the phenotypic resistance of 14 different antibiotics was determined using broth microdilution method. Odds ratio and 95 % confidence limits were calculated using multivariate logistic regression for different complexes and stages of broiler production (α = 0.05). The major AMR genes identified were aac(6′)-Iaa, aph(6)-Id, aph(3″)-Ib, blaCARB-2, sul1, tet(A), tet(B), tet(G), floR, fosA7, gyrA, and parC. The phenotypic results showed that 58 % (49/85) of isolates were resistant to at least one antibiotic class (AOR), 24 % (20/85) of isolates were resistant to at least two or more classes of antibiotics (ATR) and 6 % (5/85) of isolates were multi-drug resistant (MDR). In terms of 14 different antibiotics tested, 41 %, 39 %, 6 %, 5 % and 2 % of isolates were resistant to sulfisoxazole, tetracycline, nalidixic acid, ampicillin and ciprofloxacin respectively. Interestingly, 80 % (32/40) of Kentucky isolates showed phenotypic resistance to either tetracycline or sulfisoxazole or both. In addition, the odds of ATR Salmonella detection were 7 times (1.37 – 43.45; 95 % CL) more likely in the isolates from NAE complex as compared to conventional broiler complex (p = 0.0233). Overall, drug-resistant Salmonella were present within and surroundings of poultry production and poultry product that could potentially contaminate the final raw product. It may lead to complicated antimicrobial therapy during foodborne infections in consumers. |
| format | Article |
| id | doaj-art-a3a475d5ef4b42a2b44bc2ba9bb9e542 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-a3a475d5ef4b42a2b44bc2ba9bb9e5422025-02-06T05:10:55ZengElsevierPoultry Science0032-57912025-03-011043104855Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexesYagya Adhikari0Dianna V. Bourassa1Sabin Poudel2Matthew A. Bailey3Richard J. Buhr4Kenneth S. Macklin5Department of Poultry Science, Auburn University, Auburn, AL, USADepartment of Poultry Science, Auburn University, Auburn, AL, USADepartment of Poultry Science, Auburn University, Auburn, AL, USADepartment of Poultry Science, Auburn University, Auburn, AL, USAUSDA ARS Poultry Microbiological Safety and Processing Research Unit, Athens, GA, USADepartment of Poultry Science, Mississippi State University, Starkville, MS, USA; Corresponding author at: Department of Poultry Science, Mississippi State University, Starkville, MS 39762; USA.Antimicrobial resistance is a serious global public health concern that could endanger the efficacy of antibiotics used for the treatment of diseases in humans, animals, and plants. The objective of this study was to characterize the antimicrobial resistance (AMR) pattern of Salmonella strains isolated from different stages of both No-Antibiotics-Ever (NAE) and conventional integrated broiler complexes. ResFinder was used to identify AMR genes from whole genome sequences while the phenotypic resistance of 14 different antibiotics was determined using broth microdilution method. Odds ratio and 95 % confidence limits were calculated using multivariate logistic regression for different complexes and stages of broiler production (α = 0.05). The major AMR genes identified were aac(6′)-Iaa, aph(6)-Id, aph(3″)-Ib, blaCARB-2, sul1, tet(A), tet(B), tet(G), floR, fosA7, gyrA, and parC. The phenotypic results showed that 58 % (49/85) of isolates were resistant to at least one antibiotic class (AOR), 24 % (20/85) of isolates were resistant to at least two or more classes of antibiotics (ATR) and 6 % (5/85) of isolates were multi-drug resistant (MDR). In terms of 14 different antibiotics tested, 41 %, 39 %, 6 %, 5 % and 2 % of isolates were resistant to sulfisoxazole, tetracycline, nalidixic acid, ampicillin and ciprofloxacin respectively. Interestingly, 80 % (32/40) of Kentucky isolates showed phenotypic resistance to either tetracycline or sulfisoxazole or both. In addition, the odds of ATR Salmonella detection were 7 times (1.37 – 43.45; 95 % CL) more likely in the isolates from NAE complex as compared to conventional broiler complex (p = 0.0233). Overall, drug-resistant Salmonella were present within and surroundings of poultry production and poultry product that could potentially contaminate the final raw product. It may lead to complicated antimicrobial therapy during foodborne infections in consumers.http://www.sciencedirect.com/science/article/pii/S0032579125000926SalmonellaAntimicrobial resistanceNo-antibiotic-everConventional |
| spellingShingle | Yagya Adhikari Dianna V. Bourassa Sabin Poudel Matthew A. Bailey Richard J. Buhr Kenneth S. Macklin Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes Poultry Science Salmonella Antimicrobial resistance No-antibiotic-ever Conventional |
| title | Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes |
| title_full | Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes |
| title_fullStr | Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes |
| title_full_unstemmed | Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes |
| title_short | Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes |
| title_sort | genotypic and phenotypic characterization of antimicrobial resistance in salmonella strains isolated from both no antibiotics ever nae and conventional broiler complexes |
| topic | Salmonella Antimicrobial resistance No-antibiotic-ever Conventional |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000926 |
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