Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers
Characterization and identification of banana into their correct genome groups has been a problematic issue based on morphological and unappropriated molecular markers. In the present study, the genetic relationship between and among 28 banana (Musa spp.) accessions representing 5 genome groups (AAA...
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| Format: | Article |
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Elsevier
2025-09-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X25000848 |
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| author | Roshida Soraisham Punshi Tongbram Surendrakumar Singh Thingnam Boris Aheibam John Zothanzama Dinamani Singh Lourembam Robert Thangjam |
| author_facet | Roshida Soraisham Punshi Tongbram Surendrakumar Singh Thingnam Boris Aheibam John Zothanzama Dinamani Singh Lourembam Robert Thangjam |
| author_sort | Roshida Soraisham |
| collection | DOAJ |
| description | Characterization and identification of banana into their correct genome groups has been a problematic issue based on morphological and unappropriated molecular markers. In the present study, the genetic relationship between and among 28 banana (Musa spp.) accessions representing 5 genome groups (AAA, BB, AAB, ABB and AB) were evaluated using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. Although similar genetic relationship parameters were observed with the two markers, the higher values were generated with SRAP profiles as indicated with mean gene diversity value of 0.69 with SRAP and 0.64 with ISSR, polymorphic information content (PIC) of 0.64 with SRAP and 0.59 with ISSR, total genetic diversity (Ht) of 0.39 with SRAP and 0.34 with ISSR, gene diversity within population (Hs) of 0.19 with SRAP and 0.12 with ISSR, coefficient of gene differentiation (Gst) value of 0.51 with SRAP and 0.62 with ISSR and gene flow (Nm)value of 0.51 with SRAP and 0.31 with ISSR. The resulting dendrogram and the population structure also supports and showed the concurrence with the genetic relationship parameters based on the genome groups of the accessions studied. The present finding, also indicates the higher efficacy of SRAP markers over the ISSR markers in the discrimination of banana accessions based on their genome groups by grouping the banana accessions belonging to the different genome groups and ploidy levels. The present finding could be utilized for effective discrimination of banana accessions belonging to different genome groups. |
| format | Article |
| id | doaj-art-a3840c402b864e9cb3f2acba7161eef0 |
| institution | Kabale University |
| issn | 1687-157X |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj-art-a3840c402b864e9cb3f2acba7161eef02025-08-24T05:11:52ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2025-09-0123310054010.1016/j.jgeb.2025.100540Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markersRoshida Soraisham0Punshi Tongbram1Surendrakumar Singh Thingnam2Boris Aheibam3John Zothanzama4Dinamani Singh Lourembam5Robert Thangjam6Department of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, IndiaDepartment of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, IndiaDepartment of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, IndiaDepartment of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, IndiaDepartment of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, IndiaDepartment of Life Sciences (Botany), School of Life Sciences, Manipur University, Canchipur, Imphal 795003, IndiaDepartment of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, India; Department of Life Sciences (Botany), School of Life Sciences, Manipur University, Canchipur, Imphal 795003, India; Corresponding author at: Department of Life Sciences (Botany), School of Life Sciences, Manipur University, Canchipur, Imphal 795003, India.Characterization and identification of banana into their correct genome groups has been a problematic issue based on morphological and unappropriated molecular markers. In the present study, the genetic relationship between and among 28 banana (Musa spp.) accessions representing 5 genome groups (AAA, BB, AAB, ABB and AB) were evaluated using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. Although similar genetic relationship parameters were observed with the two markers, the higher values were generated with SRAP profiles as indicated with mean gene diversity value of 0.69 with SRAP and 0.64 with ISSR, polymorphic information content (PIC) of 0.64 with SRAP and 0.59 with ISSR, total genetic diversity (Ht) of 0.39 with SRAP and 0.34 with ISSR, gene diversity within population (Hs) of 0.19 with SRAP and 0.12 with ISSR, coefficient of gene differentiation (Gst) value of 0.51 with SRAP and 0.62 with ISSR and gene flow (Nm)value of 0.51 with SRAP and 0.31 with ISSR. The resulting dendrogram and the population structure also supports and showed the concurrence with the genetic relationship parameters based on the genome groups of the accessions studied. The present finding, also indicates the higher efficacy of SRAP markers over the ISSR markers in the discrimination of banana accessions based on their genome groups by grouping the banana accessions belonging to the different genome groups and ploidy levels. The present finding could be utilized for effective discrimination of banana accessions belonging to different genome groups.http://www.sciencedirect.com/science/article/pii/S1687157X25000848BananaMusaGenome groupSRAPISSRGenetic relationship |
| spellingShingle | Roshida Soraisham Punshi Tongbram Surendrakumar Singh Thingnam Boris Aheibam John Zothanzama Dinamani Singh Lourembam Robert Thangjam Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers Journal of Genetic Engineering and Biotechnology Banana Musa Genome group SRAP ISSR Genetic relationship |
| title | Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers |
| title_full | Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers |
| title_fullStr | Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers |
| title_full_unstemmed | Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers |
| title_short | Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers |
| title_sort | deciphering genetic relationships within and among banana musa spp genome groups using issr and srap markers |
| topic | Banana Musa Genome group SRAP ISSR Genetic relationship |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X25000848 |
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