New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice
The current study assessed the synergistic effects of PGPR and Si in alleviating drought stress in rice. Bacteria were isolated from <i>Phragmites australis</i> inhabiting an urban riverbank. Among the isolated strains, <i>Pseudomonas qingdaonensis</i> NZ 1 showed promising r...
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2025-04-01
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| author | Nazree Zainurin Muhammad Imran Shifa Shaffique Muhammad Aaqil Khan Sang-Mo Kang Md. Injamum-UL-Hoque Ashim Kumar Das Byung-Wook Yun In-Jung Lee |
| author_facet | Nazree Zainurin Muhammad Imran Shifa Shaffique Muhammad Aaqil Khan Sang-Mo Kang Md. Injamum-UL-Hoque Ashim Kumar Das Byung-Wook Yun In-Jung Lee |
| author_sort | Nazree Zainurin |
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| description | The current study assessed the synergistic effects of PGPR and Si in alleviating drought stress in rice. Bacteria were isolated from <i>Phragmites australis</i> inhabiting an urban riverbank. Among the isolated strains, <i>Pseudomonas qingdaonensis</i> NZ 1 showed promising results under in vitro drought stress induced by PEG-6000 (−0.28 MPa). To further investigate the synergistic effect of <i>Pseudomonas qingdaonensis</i> NZ 1 and silicon, a plant growth experiment was conducted comprising the control (dH<sub>2</sub>O) and plants treated with NZ 1, Si (1 mM), and NZ 1+Si under normal and drought stress conditions. The results revealed that NZ 1+Si-treated plants showed improved plant growth parameters, chlorophyll contents, relative water contents, antioxidant potential, and nutrient uptake under normal and drought conditions. Moreover, endogenous abscisic acid and jasmonic acid levels were substantially reduced, while the salicylic acid level was increased in NZ 1+Si-treated plants. Moreover, the relative expression of the ABA metabolic genes <i>OsNCED3</i> and <i>OsCYP707A6</i> and transcription factors <i>OsbZIP23</i> and <i>OsDREB1B</i> were significantly altered. Furthermore, the leaf Si, calcium, potassium, and phosphorus contents were increased in NZ 1+Si-treated drought-stressed plants, along with the upregulation of <i>OsLSi1</i>. The combined application of NZ 1 and Si offers a sustainable agricultural strategy to effectively mitigate the detrimental effects of drought. |
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| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-a356a0b46c5c49a5b5b83eac0e69b6c92025-08-20T02:33:51ZengMDPI AGMicroorganisms2076-26072025-04-01135104610.3390/microorganisms13051046New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in RiceNazree Zainurin0Muhammad Imran1Shifa Shaffique2Muhammad Aaqil Khan3Sang-Mo Kang4Md. Injamum-UL-Hoque5Ashim Kumar Das6Byung-Wook Yun7In-Jung Lee8Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaBiosafety Division, National Institute of Agriculture Science, Rural Development Administration, Jeonju 54874, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Chemical and Life Sciences, Qurtuba University, Peshawar 25000, PakistanDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaThe current study assessed the synergistic effects of PGPR and Si in alleviating drought stress in rice. Bacteria were isolated from <i>Phragmites australis</i> inhabiting an urban riverbank. Among the isolated strains, <i>Pseudomonas qingdaonensis</i> NZ 1 showed promising results under in vitro drought stress induced by PEG-6000 (−0.28 MPa). To further investigate the synergistic effect of <i>Pseudomonas qingdaonensis</i> NZ 1 and silicon, a plant growth experiment was conducted comprising the control (dH<sub>2</sub>O) and plants treated with NZ 1, Si (1 mM), and NZ 1+Si under normal and drought stress conditions. The results revealed that NZ 1+Si-treated plants showed improved plant growth parameters, chlorophyll contents, relative water contents, antioxidant potential, and nutrient uptake under normal and drought conditions. Moreover, endogenous abscisic acid and jasmonic acid levels were substantially reduced, while the salicylic acid level was increased in NZ 1+Si-treated plants. Moreover, the relative expression of the ABA metabolic genes <i>OsNCED3</i> and <i>OsCYP707A6</i> and transcription factors <i>OsbZIP23</i> and <i>OsDREB1B</i> were significantly altered. Furthermore, the leaf Si, calcium, potassium, and phosphorus contents were increased in NZ 1+Si-treated drought-stressed plants, along with the upregulation of <i>OsLSi1</i>. The combined application of NZ 1 and Si offers a sustainable agricultural strategy to effectively mitigate the detrimental effects of drought.https://www.mdpi.com/2076-2607/13/5/1046PGPRplant–microbe interactionreactive oxygen speciesphytohormonessiliconsynergism |
| spellingShingle | Nazree Zainurin Muhammad Imran Shifa Shaffique Muhammad Aaqil Khan Sang-Mo Kang Md. Injamum-UL-Hoque Ashim Kumar Das Byung-Wook Yun In-Jung Lee New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice Microorganisms PGPR plant–microbe interaction reactive oxygen species phytohormones silicon synergism |
| title | New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice |
| title_full | New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice |
| title_fullStr | New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice |
| title_full_unstemmed | New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice |
| title_short | New Insights into the Synergistic Interaction Between Pseudomonas <i>qingdaonensis</i> NZ 1 and Silicon to Mitigate Drought Stress in Rice |
| title_sort | new insights into the synergistic interaction between pseudomonas i qingdaonensis i nz 1 and silicon to mitigate drought stress in rice |
| topic | PGPR plant–microbe interaction reactive oxygen species phytohormones silicon synergism |
| url | https://www.mdpi.com/2076-2607/13/5/1046 |
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