Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis
MYB transcription factors (TFs) represent one of the largest gene families in plants, and previous studies have demonstrated their involvement in regulating anthocyanin synthesis. Eggplant is an important anthocyanin-rich solanaceae vegetable crop. In this study, a total of 219 MYB gene-family membe...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/1/12 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588362460954624 |
|---|---|
| author | Jiaqi Ai Wuhong Wang Tianhua Hu Haijiao Hu Yaqin Yan Jinglei Wang Yunzhu Wang Na Hu Hongtao Pang Chonglai Bao Qingzhen Wei |
| author_facet | Jiaqi Ai Wuhong Wang Tianhua Hu Haijiao Hu Yaqin Yan Jinglei Wang Yunzhu Wang Na Hu Hongtao Pang Chonglai Bao Qingzhen Wei |
| author_sort | Jiaqi Ai |
| collection | DOAJ |
| description | MYB transcription factors (TFs) represent one of the largest gene families in plants, and previous studies have demonstrated their involvement in regulating anthocyanin synthesis. Eggplant is an important anthocyanin-rich solanaceae vegetable crop. In this study, a total of 219 MYB gene-family members were identified using the complete and high-quality eggplant genome, comprising 105 1R-MYBs, 107 R2R3-MYBs, 5 3R-MYBs, and 2 4R-MYBs. Using phylogenetic analysis, we divided them into 33 subfamilies. Members of the SmeMYB gene family are unevenly distributed on 12 chromosomes, but are mainly concentrated at the upper and lower ends of the chromosomes. In addition, the expression levels of R2R3-MYBs in differently colored eggplant tissues (peel, calyx, stem, flower, and leaf) were investigated with RNA-seq (RNA sequencing). A total of 13 differentially expressed R2R3-MYB transcription factors related to the synthesis of anthocyanins in different eggplant tissues were screened out. <i>SmeMYB110</i>, <i>SmeMYB175</i>, and <i>SmeMYB182</i> were also found to play significant roles in this process. Furthermore, 10 MYB TFs were identified as potential genes regulating anthocyanin synthesis in different tissues. The quantitative real-time PCR (qRT-PCR) analysis results showed that SmeMYB175 was regarded as the most likely key transcription factor regulating anthocyanin synthesis in calyx. These results not only deepen our understanding of the MYB gene family in eggplant but also lay a solid foundation for further research on the regulation of SmeR2R3-MYBs in anthocyanin synthesis across diverse eggplant tissues. |
| format | Article |
| id | doaj-art-a3295c984de1406eb6229bde12bb86b7 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-a3295c984de1406eb6229bde12bb86b72025-01-24T13:34:28ZengMDPI AGHorticulturae2311-75242024-12-011111210.3390/horticulturae11010012Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin SynthesisJiaqi Ai0Wuhong Wang1Tianhua Hu2Haijiao Hu3Yaqin Yan4Jinglei Wang5Yunzhu Wang6Na Hu7Hongtao Pang8Chonglai Bao9Qingzhen Wei10College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaMYB transcription factors (TFs) represent one of the largest gene families in plants, and previous studies have demonstrated their involvement in regulating anthocyanin synthesis. Eggplant is an important anthocyanin-rich solanaceae vegetable crop. In this study, a total of 219 MYB gene-family members were identified using the complete and high-quality eggplant genome, comprising 105 1R-MYBs, 107 R2R3-MYBs, 5 3R-MYBs, and 2 4R-MYBs. Using phylogenetic analysis, we divided them into 33 subfamilies. Members of the SmeMYB gene family are unevenly distributed on 12 chromosomes, but are mainly concentrated at the upper and lower ends of the chromosomes. In addition, the expression levels of R2R3-MYBs in differently colored eggplant tissues (peel, calyx, stem, flower, and leaf) were investigated with RNA-seq (RNA sequencing). A total of 13 differentially expressed R2R3-MYB transcription factors related to the synthesis of anthocyanins in different eggplant tissues were screened out. <i>SmeMYB110</i>, <i>SmeMYB175</i>, and <i>SmeMYB182</i> were also found to play significant roles in this process. Furthermore, 10 MYB TFs were identified as potential genes regulating anthocyanin synthesis in different tissues. The quantitative real-time PCR (qRT-PCR) analysis results showed that SmeMYB175 was regarded as the most likely key transcription factor regulating anthocyanin synthesis in calyx. These results not only deepen our understanding of the MYB gene family in eggplant but also lay a solid foundation for further research on the regulation of SmeR2R3-MYBs in anthocyanin synthesis across diverse eggplant tissues.https://www.mdpi.com/2311-7524/11/1/12eggplantMYB gene familygenome-wide identificationanthocyaninRNA-seq |
| spellingShingle | Jiaqi Ai Wuhong Wang Tianhua Hu Haijiao Hu Yaqin Yan Jinglei Wang Yunzhu Wang Na Hu Hongtao Pang Chonglai Bao Qingzhen Wei Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis Horticulturae eggplant MYB gene family genome-wide identification anthocyanin RNA-seq |
| title | Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis |
| title_full | Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis |
| title_fullStr | Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis |
| title_short | Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis |
| title_sort | genome wide identification and expression analysis of eggplant reveals the key myb transcription factor involved in anthocyanin synthesis |
| topic | eggplant MYB gene family genome-wide identification anthocyanin RNA-seq |
| url | https://www.mdpi.com/2311-7524/11/1/12 |
| work_keys_str_mv | AT jiaqiai genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT wuhongwang genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT tianhuahu genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT haijiaohu genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT yaqinyan genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT jingleiwang genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT yunzhuwang genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT nahu genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT hongtaopang genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT chonglaibao genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis AT qingzhenwei genomewideidentificationandexpressionanalysisofeggplantrevealsthekeymybtranscriptionfactorinvolvedinanthocyaninsynthesis |