Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia
Highlight Research • This is the first stable isotopes study of rays from Malaysian waters • Urea extraction significantly enriched the δ15N values ("°) • Urea removal is obliged for stable isotope studies on rays • Such methodological information is critical for marine research in the re...
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| Format: | Article |
| Language: | English |
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Faculty of Fisheries and Marine Universitas Airlangga
2022-08-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/34682 |
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| author | Maizah Mohd Abdullah Wei Yien Lua Roslanzairi Mostapha Zalina Bashir Mohd Naimullah Sukree Hajisamae Fazrul Hisam |
| author_facet | Maizah Mohd Abdullah Wei Yien Lua Roslanzairi Mostapha Zalina Bashir Mohd Naimullah Sukree Hajisamae Fazrul Hisam |
| author_sort | Maizah Mohd Abdullah |
| collection | DOAJ |
| description | Highlight Research
• This is the first stable isotopes study of rays from Malaysian waters
• Urea extraction significantly enriched the δ15N values ("°)
• Urea removal is obliged for stable isotope studies on rays
• Such methodological information is critical for marine research in the region
Abstract
This is the first reported record of stable isotope values for elasmobranch rays within Malaysian waters, and serves as an important baseline methodological information for future studies investigating stable isotope values in both shark and ray species in the region. This study examined the effects of urea removal on the stable isotopes values of δ13C and δ15N in muscle tissues sampled from several elasmobranch rays species, namely Pastinachus atrus, Rhinoptera javanica, Himantura gerrardi, Himantura uarnak and Aetobatus ocellatus. Individual ray species were collected in July and August of 2018, from the coastal waters of Terengganu State, Malaysia. Urea removal was performed by soaking muscle tissue samples in deionised water for at least 24 hours before being dehydrated. The resulting stable isotope values of these samples were compared to samples that did not undergo the urea removal process. Stable isotope values were individual and species-specific, however, the effect of urea removal was significant for δ15N values but did not significantly affect the δ13C values. We conclude that removing urea from elasmobranch samples before stable isotope analysis is advisable to draw correct conclusions about the animal's diets. |
| format | Article |
| id | doaj-art-a3208cb8e9dd42ac9be31e4ec93ec33e |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-a3208cb8e9dd42ac9be31e4ec93ec33e2025-02-03T05:30:50ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592022-08-0114234034810.20473/jipk.v14i2.3468228451Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular MalaysiaMaizah Mohd Abdullah0https://orcid.org/0000-0001-7701-6623Wei Yien Lua1Roslanzairi Mostapha2Zalina Bashir3Mohd Naimullah4Sukree Hajisamae5Fazrul Hisam6Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. MalaysiaInstitute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. MalaysiaEnvironmental Tracer Application Group, Waste and environmental Technology Division, Malaysian Nuclear Agency, Kajang, Selangor, 43000. MalaysiaInstitute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. MalaysiaDepartment of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung, Taiwan, 20224. Republic of ChinaPrince of Songkla University, Pattani, 94000. ThailandFaculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. MalaysiaHighlight Research • This is the first stable isotopes study of rays from Malaysian waters • Urea extraction significantly enriched the δ15N values ("°) • Urea removal is obliged for stable isotope studies on rays • Such methodological information is critical for marine research in the region Abstract This is the first reported record of stable isotope values for elasmobranch rays within Malaysian waters, and serves as an important baseline methodological information for future studies investigating stable isotope values in both shark and ray species in the region. This study examined the effects of urea removal on the stable isotopes values of δ13C and δ15N in muscle tissues sampled from several elasmobranch rays species, namely Pastinachus atrus, Rhinoptera javanica, Himantura gerrardi, Himantura uarnak and Aetobatus ocellatus. Individual ray species were collected in July and August of 2018, from the coastal waters of Terengganu State, Malaysia. Urea removal was performed by soaking muscle tissue samples in deionised water for at least 24 hours before being dehydrated. The resulting stable isotope values of these samples were compared to samples that did not undergo the urea removal process. Stable isotope values were individual and species-specific, however, the effect of urea removal was significant for δ15N values but did not significantly affect the δ13C values. We conclude that removing urea from elasmobranch samples before stable isotope analysis is advisable to draw correct conclusions about the animal's diets.https://e-journal.unair.ac.id/JIPK/article/view/34682elasmobranchtrophic ecologybidong islandterengganusouth china sea |
| spellingShingle | Maizah Mohd Abdullah Wei Yien Lua Roslanzairi Mostapha Zalina Bashir Mohd Naimullah Sukree Hajisamae Fazrul Hisam Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia Jurnal Ilmiah Perikanan dan Kelautan elasmobranch trophic ecology bidong island terengganu south china sea |
| title | Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia |
| title_full | Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia |
| title_fullStr | Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia |
| title_full_unstemmed | Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia |
| title_short | Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia |
| title_sort | effects of urea removal on the stable isotopes i´13c and i´15n in rays from the coastal waters of peninsular malaysia |
| topic | elasmobranch trophic ecology bidong island terengganu south china sea |
| url | https://e-journal.unair.ac.id/JIPK/article/view/34682 |
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