Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel
Reproducibly assessing malaria exposure is critical for force health protection for military service members deployed to malaria-endemic regions as well as for civilians making public health decisions and evaluating malaria eradication efforts. However, malaria disease surveillance is challenged by...
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| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/10/1/13 |
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| author | Sidhartha Chaudhury Jessica S. Bolton Edwin Kamau Elke S. Bergmann-Leitner |
| author_facet | Sidhartha Chaudhury Jessica S. Bolton Edwin Kamau Elke S. Bergmann-Leitner |
| author_sort | Sidhartha Chaudhury |
| collection | DOAJ |
| description | Reproducibly assessing malaria exposure is critical for force health protection for military service members deployed to malaria-endemic regions as well as for civilians making public health decisions and evaluating malaria eradication efforts. However, malaria disease surveillance is challenged by under-reporting, natural immunity, and chemoprophylaxis, which can mask malaria exposure and lead to an underestimation of malaria prevalence. In this study, we determined the feasibility of using a serosurveillance-based approach to measure Anopheles vector exposure, Plasmodium sporozoite exposure, and blood-stage parasitemia using a multiplex serological panel. We tested post-deployment samples obtained from U.S. service members returning from regions with malaria risk to assess the potential of this serosurveillance panel. The results identified that some service members had anti-CSP antibody levels comparable to those found in endemic populations, suggesting exposure to sporozoites while those individuals were on chemoprophylaxis. We also observed isolated cases of anti-MSP1 levels that were as high as those observed in endemic populations and in CHMI studies, suggesting possible cases of clinical or subclinical parasitemia. The study demonstrated the feasibility of implementing a multiplex serology approach for conducting serosurveillance for Anopheles vector exposure and Plasmodium parasite exposure in samples collected following military deployments and its potential to support public health policies. |
| format | Article |
| id | doaj-art-a309746b3bfc449d8caae23396ace690 |
| institution | Kabale University |
| issn | 2414-6366 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj-art-a309746b3bfc449d8caae23396ace6902025-01-24T13:51:22ZengMDPI AGTropical Medicine and Infectious Disease2414-63662025-01-011011310.3390/tropicalmed10010013Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military PersonnelSidhartha Chaudhury0Jessica S. Bolton1Edwin Kamau2Elke S. Bergmann-Leitner3Bacterial and Parasitic Diseases Department, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences, Bangkok 10400, ThailandBiologics Research & Development Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USATripler Army Medical Center, Honolulu, HI 96859, USABiologics Research & Development Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USAReproducibly assessing malaria exposure is critical for force health protection for military service members deployed to malaria-endemic regions as well as for civilians making public health decisions and evaluating malaria eradication efforts. However, malaria disease surveillance is challenged by under-reporting, natural immunity, and chemoprophylaxis, which can mask malaria exposure and lead to an underestimation of malaria prevalence. In this study, we determined the feasibility of using a serosurveillance-based approach to measure Anopheles vector exposure, Plasmodium sporozoite exposure, and blood-stage parasitemia using a multiplex serological panel. We tested post-deployment samples obtained from U.S. service members returning from regions with malaria risk to assess the potential of this serosurveillance panel. The results identified that some service members had anti-CSP antibody levels comparable to those found in endemic populations, suggesting exposure to sporozoites while those individuals were on chemoprophylaxis. We also observed isolated cases of anti-MSP1 levels that were as high as those observed in endemic populations and in CHMI studies, suggesting possible cases of clinical or subclinical parasitemia. The study demonstrated the feasibility of implementing a multiplex serology approach for conducting serosurveillance for Anopheles vector exposure and Plasmodium parasite exposure in samples collected following military deployments and its potential to support public health policies.https://www.mdpi.com/2414-6366/10/1/13<i>Plasmodium</i>exposuremosquito salivaserological profileelectro-chemiluminescencebiomarker |
| spellingShingle | Sidhartha Chaudhury Jessica S. Bolton Edwin Kamau Elke S. Bergmann-Leitner Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel Tropical Medicine and Infectious Disease <i>Plasmodium</i> exposure mosquito saliva serological profile electro-chemiluminescence biomarker |
| title | Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel |
| title_full | Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel |
| title_fullStr | Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel |
| title_full_unstemmed | Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel |
| title_short | Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel |
| title_sort | assessing the feasibility of using a multiplex serological assay to conduct serosurveillance for malaria exposure in deployed military personnel |
| topic | <i>Plasmodium</i> exposure mosquito saliva serological profile electro-chemiluminescence biomarker |
| url | https://www.mdpi.com/2414-6366/10/1/13 |
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