Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress
Cadmium threatens eco-environmental security and human health, but the interaction between cadmium and microalgae cells remains unknown. This research examined the molecular detoxification mechanism of Synechocystis sp. to cadmium. The results indicated that cadmium stress significantly inhibited ch...
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Elsevier
2025-07-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325007250 |
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| author | Gang Ruan Changzi Liu Gaofei Song Jing Qian Tao Bao Yafei Zhao Shaoqiang Sun Dong Wan Wujuan Mi Man He Bin Hu Yonghong Bi |
| author_facet | Gang Ruan Changzi Liu Gaofei Song Jing Qian Tao Bao Yafei Zhao Shaoqiang Sun Dong Wan Wujuan Mi Man He Bin Hu Yonghong Bi |
| author_sort | Gang Ruan |
| collection | DOAJ |
| description | Cadmium threatens eco-environmental security and human health, but the interaction between cadmium and microalgae cells remains unknown. This research examined the molecular detoxification mechanism of Synechocystis sp. to cadmium. The results indicated that cadmium stress significantly inhibited chlorophyll a content and maximum photochemical quantum yield (Fv/Fm), with EC50 of 0.50 mg L−1. The differentially expressed genes/proteins (DEGs/DEPs) were significantly enriched in pathways of two-component system, translation, nucleotide metabolism, ribosome, photosynthesis and chlorophyll synthesis. 1073 DEGs and 338 DEPs were identified, and 84 DEGs/DEPs with consistent expression trends were obtained. Foldchange of Sll1725 ranked fourth in DEGs/DEPs but its function was unexplored. Phylogenetic analysis and 3D structure identified Sll1725 as an ABC transporter and molecular simulation determined its cadmium-efflux function. Under 0.50 mg L−1 cadmium stress, Δsll1725 had lower growth and Fv/Fm values than the wild-type. Meanwhile, the intracellular cadmium in Δsll1725 was higher, indicating that Sll1725 mitigated cadmium toxicity by efflux. The duckweed with overexpressed sll1725 exhibited cadmium tolerance. It could be deduced that Sll1725, belonged to ABC transporters, which played an important detoxification mechanism. These mutants might possess the potential for bioremediation. This study provides a basis for applying algal genetic resources in cadmium pollution treatment. |
| format | Article |
| id | doaj-art-a2f35c3bc7914b2e97ab5e7788e08df1 |
| institution | DOAJ |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-a2f35c3bc7914b2e97ab5e7788e08df12025-08-20T02:39:38ZengElsevierEcotoxicology and Environmental Safety0147-65132025-07-0130011838910.1016/j.ecoenv.2025.118389Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stressGang Ruan0Changzi Liu1Gaofei Song2Jing Qian3Tao Bao4Yafei Zhao5Shaoqiang Sun6Dong Wan7Wujuan Mi8Man He9Bin Hu10Yonghong Bi11State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Carbon Neutral R&D Center, China Railway Hi-Tech Industry Corporation Limited, Beijing 100049, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Corresponding author.Cadmium threatens eco-environmental security and human health, but the interaction between cadmium and microalgae cells remains unknown. This research examined the molecular detoxification mechanism of Synechocystis sp. to cadmium. The results indicated that cadmium stress significantly inhibited chlorophyll a content and maximum photochemical quantum yield (Fv/Fm), with EC50 of 0.50 mg L−1. The differentially expressed genes/proteins (DEGs/DEPs) were significantly enriched in pathways of two-component system, translation, nucleotide metabolism, ribosome, photosynthesis and chlorophyll synthesis. 1073 DEGs and 338 DEPs were identified, and 84 DEGs/DEPs with consistent expression trends were obtained. Foldchange of Sll1725 ranked fourth in DEGs/DEPs but its function was unexplored. Phylogenetic analysis and 3D structure identified Sll1725 as an ABC transporter and molecular simulation determined its cadmium-efflux function. Under 0.50 mg L−1 cadmium stress, Δsll1725 had lower growth and Fv/Fm values than the wild-type. Meanwhile, the intracellular cadmium in Δsll1725 was higher, indicating that Sll1725 mitigated cadmium toxicity by efflux. The duckweed with overexpressed sll1725 exhibited cadmium tolerance. It could be deduced that Sll1725, belonged to ABC transporters, which played an important detoxification mechanism. These mutants might possess the potential for bioremediation. This study provides a basis for applying algal genetic resources in cadmium pollution treatment.http://www.sciencedirect.com/science/article/pii/S0147651325007250CadmiumAlgaeTranscriptomeProteomeDetoxification mechanism |
| spellingShingle | Gang Ruan Changzi Liu Gaofei Song Jing Qian Tao Bao Yafei Zhao Shaoqiang Sun Dong Wan Wujuan Mi Man He Bin Hu Yonghong Bi Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress Ecotoxicology and Environmental Safety Cadmium Algae Transcriptome Proteome Detoxification mechanism |
| title | Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress |
| title_full | Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress |
| title_fullStr | Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress |
| title_full_unstemmed | Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress |
| title_short | Sll1725, an ABC transporter in Synechocystis sp. PCC 6803 for the detoxification of cadmium ion stress |
| title_sort | sll1725 an abc transporter in synechocystis sp pcc 6803 for the detoxification of cadmium ion stress |
| topic | Cadmium Algae Transcriptome Proteome Detoxification mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325007250 |
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